(Circular) (Six-base) MAPSORT of: pFN21AE0561.seq Check: 5083 from: 1 to: 5704 pFN21AE0561 5704 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2471 3047 1937 Size: 534 576 4594 Fragments arranged by size: 4594 576 534 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 2297 2627 3767 5151 5419 1632 Size: 424 241 330 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 424 330 268 241 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 2616 4535 2616 Size: 1919 3785 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2992 3447 5019 2992 Size: 455 1572 3677 Fragments arranged by size: 3677 1572 455 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 2653 3025 4480 618 Size: 325 217 627 445 421 372 1455 1842 Fragments arranged by size: 1842 1455 627 445 421 372 325 217 BanII G_rGCy'C Cuts at: 1391 2337 3023 1391 Size: 946 686 4072 Fragments arranged by size: 4072 946 686 BbeI G_GCGC'C Cuts at: 2236 2236 Size: 5704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2192 2285 928 Size: 971 293 93 4347 Fragments arranged by size: 4347 971 293 93 BbvCI CC'TCA_GC Cuts at: 2193 2193 Size: 5704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3895 3929 1903 Size: 34 50 34 1874 34 3678 Fragments arranged by size: 3678 1874 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 2698 1548 Size: 1150 4554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 4315 136 Size: 107 122 71 631 605 700 1943 1525 Fragments arranged by size: 1943 1525 700 631 605 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 3765 4921 1790 Size: 1975 1156 2573 Fragments arranged by size: 2573 1975 1156 BmgBI CAC'GTC Cuts at: 1318 2792 1318 Size: 1474 4230 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2435 3435 4393 Size: 416 53 83 399 24 1018 1000 958 Cuts at: 4393 442 Size: 1753 Fragments arranged by size: 1753 1018 1000 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3014 4364 1239 Size: 1775 1350 2579 Fragments arranged by size: 2579 1775 1350 Bpu10I CC'TnA_GC Cuts at: 2193 2193 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2252 3829 4694 4992 2015 Size: 237 1577 865 298 2727 Fragments arranged by size: 2727 1577 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2975 4367 882 Size: 617 1476 1392 2219 Fragments arranged by size: 2219 1476 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 2939 3495 493 Size: 711 1735 556 2702 Fragments arranged by size: 2702 1735 711 556 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2233 3511 3893 4528 Size: 53 83 186 936 700 1278 382 635 Cuts at: 4528 275 Size: 1451 Fragments arranged by size: 1451 1278 936 700 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2616 4137 4535 4809 4956 5367 2616 Size: 1521 398 274 147 411 2953 Fragments arranged by size: 2953 1521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5704 BseYI C'CCAG_C Cuts at: 2297 4907 5403 2297 Size: 2610 496 2598 Fragments arranged by size: 2610 2598 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2593 1163 Size: 573 857 4274 Fragments arranged by size: 4274 857 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 3154 3915 4943 1540 Size: 275 186 205 948 761 1028 2301 Fragments arranged by size: 2301 1028 948 761 275 205 186 BsiHKAI G_wGCw'C Cuts at: 3765 3850 4921 3765 Size: 85 1071 4548 Fragments arranged by size: 4548 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 BsmBI CGTCTCn'nnnn_ Cuts at: 2678 2678 Size: 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 3023 3765 3850 4921 1391 Size: 399 547 686 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 686 547 399 85 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 2284 3593 5323 2284 Size: 1309 1730 2665 Fragments arranged by size: 2665 1730 1309 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2674 3059 844 Size: 1830 385 3489 Fragments arranged by size: 3489 1830 385 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2616 4348 4535 1700 Size: 110 398 408 1732 187 2869 Fragments arranged by size: 2869 1732 408 398 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 2653 794 Size: 1859 3845 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2330 2330 Size: 5704 BstYI r'GATC_y Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699 Size: 1062 367 385 17 1452 86 12 346 Cuts at: 5699 1972 Size: 1977 Fragments arranged by size: 1977 1452 1062 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2377 2377 Size: 5704 BtgI C'CryG_G Cuts at: 513 1065 2344 513 Size: 552 1279 3873 Fragments arranged by size: 3873 1279 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2083 2118 529 Size: 35 1519 35 4115 Fragments arranged by size: 4115 1519 35 35 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2356 3151 Size: 54 1425 50 275 18 373 153 795 Cuts at: 3151 4040 5669 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 795 373 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2203 3151 1537 Size: 275 391 948 4090 Fragments arranged by size: 4090 948 391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3634 1353 Size: 2281 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2297 2627 3014 3767 4364 5151 Size: 393 424 241 330 387 753 597 787 Cuts at: 5151 5419 1239 Size: 268 1524 Fragments arranged by size: 1524 787 753 597 424 393 387 330 268 241 EcoNI CCTnn'n_nnAGG Cuts at: 2196 2221 2196 Size: 25 5679 EcoO109I rG'GnC_Cy Cuts at: 2026 2478 2815 2026 Size: 452 337 4915 Fragments arranged by size: 4915 452 337 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 2633 4210 2633 Size: 1577 4127 FspAI rTGC'GCAy Cuts at: 2633 2633 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2236 4851 1678 Size: 558 2615 2531 Fragments arranged by size: 2615 2531 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2869 2901 4345 4377 4419 Size: 32 135 32 1297 32 1444 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 1444 1297 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 2731 3048 3294 669 Size: 988 281 172 621 317 246 3079 Fragments arranged by size: 3079 988 621 317 281 246 172 HindIII A'AGCT_T Cuts at: 748 2270 748 Size: 1522 4182 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2232 2232 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2358 10 Size: 54 1425 343 526 3356 Fragments arranged by size: 3356 1425 526 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2549 3662 4021 4180 518 Size: 648 1162 221 1113 359 159 2042 Fragments arranged by size: 2042 1162 1113 648 359 221 159 MspA1I CmG'CkG Cuts at: 1606 3120 3797 4945 5190 1606 Size: 1514 677 1148 245 2120 Fragments arranged by size: 2120 1514 1148 677 245 NarI GG'CG_CC Cuts at: 2233 2233 Size: 5704 NcoI C'CATG_G Cuts at: 513 1065 2344 513 Size: 552 1279 3873 Fragments arranged by size: 3873 1279 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 2203 3151 2203 Size: 948 4756 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2758 2992 1108 Size: 1650 234 3820 Fragments arranged by size: 3820 1650 234 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2026 Size: 5704 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 3056 830 Size: 2226 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SexAI A'CCwGG_T Cuts at: 2118 2118 Size: 5704 SfcI C'TryA_G Cuts at: 826 1045 2248 2472 2827 3052 4187 4868 5059 Size: 219 1203 224 355 225 1135 681 191 Cuts at: 5059 826 Size: 1471 Fragments arranged by size: 1471 1203 1135 681 355 225 224 219 191 SfoI GGC'GCC Cuts at: 2234 2234 Size: 5704 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 2267 3808 4709 4971 820 Size: 197 926 87 237 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 262 237 197 87 SnaBI TAC'GTA Cuts at: 493 2939 3495 493 Size: 2446 556 2702 Fragments arranged by size: 2702 2446 556 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2375 1517 Size: 858 4846 StyI C'CwwG_G Cuts at: 513 1065 2344 3143 513 Size: 552 1279 799 3074 Fragments arranged by size: 3074 1279 799 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3731 3748 3901 4086 4531 325 Size: 1255 2151 17 153 185 445 1498 Fragments arranged by size: 2151 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2351 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 2747 3833 2747 Size: 1086 4618 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI