Restriction map for
pFN21AE0561
(Linear) (Six-base) MAPSORT of: KIEE0561.seq Check: 1985 from: 1 to: 999
KIEE0561.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 466 999
Size: 466 533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 292 622 999
Size: 51 241 330 377
Fragments arranged by size:
377 330 241 51
AgeI A'CCGG_T
Cuts at: 0 611 999
Size: 611 388
AlwNI CAG_nnn'CTG
Cuts at: 0 987 999
Size: 987 12
BanI G'GyrC_C
Cuts at: 0 227 648 999
Size: 227 421 351
Fragments arranged by size:
421 351 227
BanII G_rGCy'C
Cuts at: 0 332 999
Size: 332 667
BbeI G_GCGC'C
Cuts at: 0 231 999
Size: 231 768
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 280 999
Size: 187 93 719
Fragments arranged by size:
719 187 93
BbvCI CC'TCA_GC
Cuts at: 0 188 999
Size: 188 811
BclI T'GATC_A
Cuts at: 0 693 999
Size: 693 306
BglI GCCn_nnn'nGGC
Cuts at: 0 367 999
Size: 367 632
BmgBI CAC'GTC
Cuts at: 0 787 999
Size: 787 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 999
Size: 430 569
Bpu10I CC'TnA_GC
Cuts at: 0 188 999
Size: 188 811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 247 999
Size: 10 237 752
Fragments arranged by size:
752 237 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 970 999
Size: 970 29
BsaAI yAC'GTr
Cuts at: 0 934 999
Size: 934 65
BsaHI Gr'CG_yC
Cuts at: 0 228 999
Size: 228 771
BsaWI w'CCGG_w
Cuts at: 0 611 999
Size: 611 388
BseYI C'CCAG_C
Cuts at: 0 292 999
Size: 292 707
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 999
Size: 588 411
BsiEI CG_ry'CG
Cuts at: 0 201 999
Size: 201 798
BsmBI CGTCTCn'nnnn_
Cuts at: 0 673 999
Size: 673 326
Bsp1286I G_dGCh'C
Cuts at: 0 332 999
Size: 332 667
BspHI T'CATG_A
Cuts at: 0 279 999
Size: 279 720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 669 999
Size: 669 330
BsrFI r'CCGG_y
Cuts at: 0 203 611 999
Size: 203 408 388
Fragments arranged by size:
408 388 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 999
Size: 648 351
BstXI CCAn_nnnn'nTGG
Cuts at: 0 325 999
Size: 325 674
Bsu36I CC'TnA_GG
Cuts at: 0 372 999
Size: 372 627
BtgI C'CryG_G
Cuts at: 0 339 999
Size: 339 660
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 78 113 999
Size: 78 35 886
Fragments arranged by size:
886 78 35
EaeI y'GGCC_r
Cuts at: 0 198 351 999
Size: 198 153 648
Fragments arranged by size:
648 198 153
EagI C'GGCC_G
Cuts at: 0 198 999
Size: 198 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 292 622 999
Size: 51 241 330 377
Fragments arranged by size:
377 330 241 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 191 216 999
Size: 191 25 783
Fragments arranged by size:
783 191 25
EcoO109I rG'GnC_Cy
Cuts at: 0 21 473 810 999
Size: 21 452 337 189
Fragments arranged by size:
452 337 189 21
FspI TGC'GCA
Cuts at: 0 628 999
Size: 628 371
FspAI rTGC'GCAy
Cuts at: 0 628 999
Size: 628 371
HaeII r_GCGC'y
Cuts at: 0 231 999
Size: 231 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 864 896 999
Size: 864 32 103
Fragments arranged by size:
864 103 32
HincII GTy'rAC
Cuts at: 0 105 726 999
Size: 105 621 273
Fragments arranged by size:
621 273 105
HindIII A'AGCT_T
Cuts at: 0 265 999
Size: 265 734
KasI G'GCGC_C
Cuts at: 0 227 999
Size: 227 772
MscI TGG'CCA
Cuts at: 0 353 999
Size: 353 646
MslI CAynn'nnrTG
Cuts at: 0 323 544 999
Size: 323 221 455
Fragments arranged by size:
455 323 221
NarI GG'CG_CC
Cuts at: 0 228 999
Size: 228 771
NcoI C'CATG_G
Cuts at: 0 339 999
Size: 339 660
NotI GC'GGCC_GC
Cuts at: 0 198 999
Size: 198 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 987 999
Size: 753 234 12
Fragments arranged by size:
753 234 12
PpuMI rG'GwC_Cy
Cuts at: 0 21 999
Size: 21 978
SexAI A'CCwGG_T
Cuts at: 0 113 999
Size: 113 886
SfcI C'TryA_G
Cuts at: 0 243 467 822 999
Size: 243 224 355 177
Fragments arranged by size:
355 243 224 177
SfoI GGC'GCC
Cuts at: 0 229 999
Size: 229 770
SmlI C'TyrA_G
Cuts at: 0 25 262 999
Size: 25 237 737
Fragments arranged by size:
737 237 25
SnaBI TAC'GTA
Cuts at: 0 934 999
Size: 934 65
StuI AGG'CCT
Cuts at: 0 370 999
Size: 370 629
StyI C'CwwG_G
Cuts at: 0 339 999
Size: 339 660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 999
Size: 346 653
XmnI GAAnn'nnTTC
Cuts at: 0 742 999
Size: 742 257
Enzymes that do cut:
AccI AcuI AgeI AlwNI BanI BanII BbeI BbsI
BbvCI BclI BglI BmgBI BmrI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsmBI Bsp1286I
BspHI BspMI BsrFI BstAPI BstXI Bsu36I BtgI CspCI
EaeI EagI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I HincII HindIII KasI MscI MslI NarI NcoI
NotI PflMI PpuMI SexAI SfcI SfoI SmlI SnaBI
StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmtI BplI BpmI BsaBI BsaXI
BseRI BsiHKAI BsiWI BsmI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI BtsI
ClaI DraI DraIII DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI HpaI KpnI MfeI MluI MmeI
MspA1I NaeI NdeI NgoMIV NheI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI