(Circular) (Six-base) MAPSORT of: pFN21AE0562.seq Check: 5238 from: 1 to: 5689 pFN21AE0562 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 3032 1937 Size: 169 926 4594 Fragments arranged by size: 4594 926 169 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3752 5136 5404 1632 Size: 653 1467 1384 268 1917 Fragments arranged by size: 1917 1467 1384 653 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2041 2692 3432 5004 2041 Size: 651 740 1572 2726 Fragments arranged by size: 2726 1572 740 651 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 3010 4465 618 Size: 325 217 627 549 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 549 325 217 BanII G_rGCy'C Cuts at: 1391 2641 2805 3008 1391 Size: 1250 164 203 4072 Fragments arranged by size: 4072 1250 203 164 BbeI G_GCGC'C Cuts at: 2340 2340 Size: 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 2273 2384 2758 928 Size: 971 281 93 111 374 3859 Fragments arranged by size: 3859 971 374 281 111 93 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2593 2627 3880 3914 1903 Size: 34 656 34 1253 34 3678 Fragments arranged by size: 3678 1253 656 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 2683 1548 Size: 1135 4554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2504 3106 2504 Size: 602 5087 Bme1580I G_kGCm'C Cuts at: 1790 2574 3750 4906 1790 Size: 784 1176 1156 2573 Fragments arranged by size: 2573 1176 1156 784 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2808 3420 4378 Size: 416 53 83 399 24 1391 612 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1391 958 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2612 2800 4349 1239 Size: 1340 33 188 1549 2579 Fragments arranged by size: 2579 1549 1340 188 33 Bpu10I CC'TnA_GC Cuts at: 2286 2286 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2960 4352 882 Size: 617 1461 1392 2219 Fragments arranged by size: 2219 1461 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2337 3496 3878 4513 Size: 53 83 186 936 804 1159 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1159 936 804 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 1186 Size: 1202 4487 BseYI C'CCAG_C Cuts at: 4892 5388 4892 Size: 496 5193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2338 2906 1163 Size: 573 602 568 3946 Fragments arranged by size: 3946 602 573 568 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2292 2474 3750 3835 4906 2292 Size: 182 1276 85 1071 3075 Fragments arranged by size: 3075 1276 1071 182 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2474 2574 2641 2805 3008 3750 Size: 399 502 182 100 67 164 203 742 Cuts at: 3750 3835 4906 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 399 203 182 164 100 85 67 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 3044 844 Size: 1274 926 3489 Fragments arranged by size: 3489 1274 926 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 2715 96 Size: 2619 3070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2525 3749 4761 2525 Size: 1224 1012 3453 Fragments arranged by size: 3453 1224 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2352 2569 794 Size: 1558 217 3914 Fragments arranged by size: 3914 1558 217 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2516 2516 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2085 2430 3019 3386 3771 3788 5240 5326 Size: 113 345 589 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 589 385 367 346 345 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2317 2509 513 Size: 552 939 313 192 3693 Fragments arranged by size: 3693 939 552 313 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2060 2852 3205 3999 4019 2060 Size: 792 353 794 20 3730 Fragments arranged by size: 3730 794 792 353 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 2406 2457 Size: 54 1425 50 275 18 299 277 51 Cuts at: 2457 3136 4025 5654 8 Size: 679 889 1629 43 Fragments arranged by size: 1629 1425 889 679 299 277 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2726 4276 4650 4796 387 Size: 1064 138 1137 1550 374 146 1280 Fragments arranged by size: 1550 1280 1137 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2579 2612 2800 3752 4349 5136 Size: 393 653 294 33 188 952 597 787 Cuts at: 5136 5404 1239 Size: 268 1524 Fragments arranged by size: 1524 952 787 653 597 393 294 268 188 33 EcoNI CCTnn'n_nnAGG Cuts at: 2209 2440 2209 Size: 231 5458 EcoO109I rG'GnC_Cy Cuts at: 2275 2557 2275 Size: 282 5407 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2452 2452 Size: 5689 FspI TGC'GCA Cuts at: 2618 4195 2618 Size: 1577 4112 FspAI rTGC'GCAy Cuts at: 2618 2618 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2340 4836 1678 Size: 662 2496 2531 Fragments arranged by size: 2531 2496 662 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373 Size: 32 135 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 2779 748 Size: 2031 3658 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2336 2336 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2459 10 Size: 54 1425 343 576 51 3240 Fragments arranged by size: 3240 1425 576 343 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2125 2316 2537 3647 4006 4165 518 Size: 648 959 191 221 1110 359 159 2042 Fragments arranged by size: 2042 1110 959 648 359 221 191 159 MspA1I CmG'CkG Cuts at: 1606 3105 3782 4930 5175 1606 Size: 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 245 NarI GG'CG_CC Cuts at: 2337 2337 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 2004 2317 2509 513 Size: 552 939 313 192 3693 Fragments arranged by size: 3693 939 552 313 192 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2082 2884 1108 Size: 974 802 3913 Fragments arranged by size: 3913 974 802 PfoI T'CCnGG_A Cuts at: 2163 2163 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2275 Size: 5689 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 2055 3041 830 Size: 1225 986 3478 Fragments arranged by size: 3478 1225 986 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2051 3037 4172 4853 5044 826 Size: 219 1006 986 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 986 681 219 191 SfoI GGC'GCC Cuts at: 2338 2338 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2821 1517 Size: 1304 4385 StyI C'CwwG_G Cuts at: 513 1065 2004 2317 2509 3128 513 Size: 552 939 313 192 619 3074 Fragments arranged by size: 3074 939 619 552 313 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 3716 3733 3886 4071 4516 325 Size: 1255 960 1176 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1176 960 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 3935 5647 Size: 274 80 33 51 1443 738 1220 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1220 738 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2206 2238 5649 5681 2206 Size: 32 3411 32 2214 Fragments arranged by size: 3411 2214 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 2381 3818 2381 Size: 1437 4252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI