(Circular) (Six-base) MAPSORT of: pFN21AB8664.seq Check: 3429 from: 1 to: 5932 pFN21AB8664 5932 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2167 3742 278 Size: 53 83 186 936 631 1575 2468 Fragments arranged by size: 2468 1575 936 631 186 83 53 AccI GT'mk_AC Cuts at: 1937 3069 3153 3275 1937 Size: 1132 84 122 4594 Fragments arranged by size: 4594 1132 122 84 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2857 2954 3995 5379 5647 1632 Size: 1225 97 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1225 1041 268 97 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 4831 5808 1203 Size: 3628 977 1327 Fragments arranged by size: 3628 1327 977 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5932 AlwNI CAG_nnn'CTG Cuts at: 3675 5247 3675 Size: 1572 4360 ApaI G_GGCC'C Cuts at: 3191 3191 Size: 5932 ApaLI G'TGCA_C Cuts at: 3989 5145 3989 Size: 1156 4776 ApoI r'AATT_y Cuts at: 1493 2324 2510 3241 3467 3619 5728 1493 Size: 831 186 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 731 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2969 3257 1870 Size: 73 1026 288 4545 Fragments arranged by size: 4545 1026 288 73 BamHI G'GATC_C Cuts at: 3262 3629 3262 Size: 367 5565 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2369 3253 4708 618 Size: 325 217 627 582 884 1455 1842 Fragments arranged by size: 1842 1455 884 627 582 325 217 BanII G_rGCy'C Cuts at: 1391 2441 2801 2977 3138 3191 3251 1391 Size: 1050 360 176 161 53 60 4072 Fragments arranged by size: 4072 1050 360 176 161 60 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2686 2996 928 Size: 971 787 310 3864 Fragments arranged by size: 3864 971 787 310 BbvCI CC'TCA_GC Cuts at: 2097 2891 2097 Size: 794 5138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 1903 Size: 34 2186 34 3678 Fragments arranged by size: 3678 2186 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3824 5034 3824 Size: 1210 4722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4543 136 Size: 107 122 71 631 605 2871 1525 Fragments arranged by size: 2871 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 3191 3993 5149 1790 Size: 1401 802 1156 2573 Fragments arranged by size: 2573 1401 1156 802 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 3663 4621 Size: 416 53 83 399 24 1652 594 958 Cuts at: 4621 442 Size: 1753 Fragments arranged by size: 1753 1652 958 594 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 4592 1239 Size: 866 2487 2579 Fragments arranged by size: 2579 2487 866 Bpu10I CC'TnA_GC Cuts at: 2097 2129 2891 2097 Size: 32 762 5138 Fragments arranged by size: 5138 762 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 4057 4922 5220 2159 Size: 1898 865 298 2871 Fragments arranged by size: 2871 1898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2932 4595 882 Size: 617 1433 1663 2219 Fragments arranged by size: 2219 1663 1433 617 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 2816 5926 2816 Size: 3110 2822 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2164 3739 4121 4756 Size: 53 83 186 936 631 1575 382 635 Cuts at: 4756 275 Size: 1451 Fragments arranged by size: 1575 1451 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3159 4365 4763 5037 5184 5595 3159 Size: 1206 398 274 147 411 3496 Fragments arranged by size: 3496 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3212 3242 1178 Size: 30 165 30 1809 30 3868 Fragments arranged by size: 3868 1809 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2483 2704 2848 3233 1186 Size: 838 459 221 144 385 3885 Fragments arranged by size: 3885 838 459 385 221 144 BseYI C'CCAG_C Cuts at: 2801 3021 5135 5631 2801 Size: 220 2114 496 3102 Fragments arranged by size: 3102 2114 496 220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2120 1163 Size: 573 384 4975 Fragments arranged by size: 4975 573 384 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2913 3993 4078 5149 2913 Size: 1080 85 1071 3696 Fragments arranged by size: 3696 1080 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3536 3443 Size: 93 5839 BsmBI CGTCTCn'nnnn_ Cuts at: 2246 3127 2246 Size: 881 5051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2441 2801 2913 2977 3138 3191 3251 Size: 399 651 360 112 64 161 53 60 Cuts at: 3251 3993 4078 5149 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 360 161 112 85 64 60 53 BspEI T'CCGG_A Cuts at: 5595 5595 Size: 5932 BspHI T'CATG_A Cuts at: 2728 3821 5551 2728 Size: 1093 1730 3109 Fragments arranged by size: 3109 1730 1093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3287 844 Size: 2443 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3819 1394 Size: 429 1996 3507 Fragments arranged by size: 3507 1996 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4427 4601 65 Size: 1183 3179 174 1396 Fragments arranged by size: 3179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2577 4576 4763 1700 Size: 110 767 1999 187 2869 Fragments arranged by size: 2869 1999 767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5932 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 2548 3992 5004 2548 Size: 1444 1012 3476 Fragments arranged by size: 3476 1444 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5932 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 2107 1184 Size: 923 5009 BstYI r'GATC_y Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927 Size: 1290 367 385 17 1452 86 12 346 Cuts at: 5927 1972 Size: 1977 Fragments arranged by size: 1977 1452 1290 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3191 513 Size: 552 939 1187 3254 Fragments arranged by size: 3254 1187 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5193 Fragments arranged by size: 5193 683 56 BtsI GCAGTG_nn' Cuts at: 2035 2044 2835 3448 4242 4262 2035 Size: 9 791 613 794 20 3705 Fragments arranged by size: 3705 794 791 613 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DraIII CAC_nnn'GTG Cuts at: 2305 2305 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897 Size: 54 1425 50 275 18 1549 889 1629 Cuts at: 5897 8 Size: 43 Fragments arranged by size: 1629 1549 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 2930 3862 1353 Size: 995 582 932 3423 Fragments arranged by size: 3423 995 932 582 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2686 4519 4893 5039 387 Size: 1064 138 1097 1833 374 146 1280 Fragments arranged by size: 1833 1280 1097 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2857 2954 3995 4592 5379 5647 Size: 393 473 752 97 1041 597 787 268 Cuts at: 5647 1239 Size: 1524 Fragments arranged by size: 1524 1041 787 752 597 473 393 268 97 EcoRI G'AATT_C Cuts at: 2324 2510 3241 3619 2324 Size: 186 731 378 4637 Fragments arranged by size: 4637 731 378 186 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 5079 1678 Size: 3401 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4573 4605 4647 4679 1373 Size: 32 135 32 3001 32 42 32 2626 Fragments arranged by size: 3001 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3130 3276 3522 669 Size: 988 281 1192 146 246 3079 Fragments arranged by size: 3079 1192 988 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5046 5230 1305 Size: 3741 184 2007 Fragments arranged by size: 3741 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 2498 3890 4249 4408 518 Size: 648 1141 191 1392 359 159 2042 Fragments arranged by size: 2042 1392 1141 648 359 191 159 MspA1I CmG'CkG Cuts at: 1606 2055 2853 2865 3348 4025 5173 5418 1606 Size: 449 798 12 483 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 483 449 245 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4441 Fragments arranged by size: 4441 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 2609 3152 3290 4835 1132 Size: 97 1380 543 138 1545 2229 Fragments arranged by size: 2229 1545 1380 543 138 97 PasI CC'CwG_GG Cuts at: 1348 2463 1348 Size: 1115 4817 PciI A'CATG_T Cuts at: 4831 4831 Size: 5932 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5932 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 5932 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3200 1613 Size: 1587 4345 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PspOMI G'GGCC_C Cuts at: 3187 3187 Size: 5932 PstI C_TGCA'G Cuts at: 830 2460 2830 3088 3284 830 Size: 1630 370 258 196 3478 Fragments arranged by size: 3478 1630 370 258 196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 2853 2853 Size: 5932 RsrII CG'GwC_CG Cuts at: 3162 3162 Size: 5932 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SfcI C'TryA_G Cuts at: 826 1045 2456 2826 3066 3084 3280 4415 5096 Size: 219 1411 370 240 18 196 1135 681 Cuts at: 5096 5287 826 Size: 191 1471 Fragments arranged by size: 1471 1411 1135 681 370 240 219 196 191 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 2971 3259 1872 Size: 1099 288 4545 Fragments arranged by size: 4545 1099 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 4036 4937 5199 820 Size: 197 926 231 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 3290 1132 Size: 2158 3774 SspI AAT'ATT Cuts at: 5 52 3856 5 Size: 47 3804 2081 Fragments arranged by size: 3804 2081 47 StuI AGG'CCT Cuts at: 1517 2399 1517 Size: 882 5050 StyI C'CwwG_G Cuts at: 513 1065 2004 3371 513 Size: 552 939 1367 3074 Fragments arranged by size: 3074 1367 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2637 3959 3976 4129 4314 4759 325 Size: 1255 1057 1322 17 153 185 445 1498 Fragments arranged by size: 1498 1322 1255 1057 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4178 5890 96 Size: 274 80 33 51 1443 2201 1712 138 Fragments arranged by size: 2201 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3212 3244 5892 5924 3212 Size: 32 2648 32 3220 Fragments arranged by size: 3220 2648 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5932 XmaI C'CCGG_G Cuts at: 1870 2969 3257 1870 Size: 1099 288 4545 Fragments arranged by size: 4545 1099 288 XmnI GAAnn'nnTTC Cuts at: 2993 4061 2993 Size: 1068 4864 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2165 3740 276 Size: 53 83 186 936 631 1575 2468 Fragments arranged by size: 2468 1575 936 631 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI