################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluNI MroI MscI MseI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 170 CGCG CG^CG AciI 110 GCGG CCGC(-3/-1) AflIII 169 ACGCGT A^C[GA][CT]GT AluI 130 AGCT AG^CT BamHI 5 GGATCC G^GATCC BanI 45 GGTGCC G^G[CT][GA]CC BcgI 178 CGAAAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 126 GCTCAGC GC^T[ACGT]AGC Bpu1102I 126 GCTCAGC GC^T[ACGT]AGC BsiEI 111 CGGCCG CG[GA][CT]^CG BsrFI 113 GCCGGT [GA]^CCGG[CT] BssKI 55 CCAGG ^CC[ACGT]GG BstNI 55 CCAGG CC^[AT]GG BstUI 170 CGCG CG^CG BstYI 5 GGATCC [GA]^GATC[CT] CelII 126 GCTCAGC GC^T[ACGT]AGC Cfr10I 113 GCCGGT [GA]^CCGG[CT] DdeI 127 CTCAG C^T[ACGT]AG EagI 111 CGGCCG C^GGCCG EarI 31 CTCTTC CTCTTC(1/4) EclXI 111 CGGCCG C^GGCCG Eco52I 111 CGGCCG C^GGCCG EcoRII 55 CCAGG ^CC[AT]GG HapII 114 CCGG C^CGG HgaI 168 GACGC GACGC(5/10) HpaII 114 CCGG C^CGG Ksp632I 31 CTCTTC CTCTTC(1/4) MflI 5 GGATCC [GA]^GATC[CT] MluI 169 ACGCGT A^CGCGT MspA1I 129 CAGCTG C[AC]G^C[GT]G MspI 114 CCGG C^CGG MvaI 55 CCAGG CC^[AT]GG MvnI 170 CGCG CG^CG MwoI 176 GCCGAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 61 CATG CATG^ PflMI 55 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 101 GAGTC GAGTC(4/5) PvuII 129 CAGCTG CAG^CTG SapI 30 GCTCTTC GCTCTTC(1/4) ScrFI 55 CCAGG CC^[ACGT]GG SspI 89 AATATT AAT^ATT TaqI 166 TCGA T^CGA TfiI 68 GAATC G^A[AT]TC TthHB8I 166 TCGA T^CGA Van91I 55 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 5 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 6 GATCC GGATC(4/5) 5 GGATC AspHI 124 GTGCTC G[AT]GC[AT]^C 162 GTGCTC BbvI 131 GCTGC GCAGC(8/12) 185 GCTGC BmyI 124 GTGCTC G[AGT]GC[ACT]^C 162 GTGCTC BsiHKAI 124 GTGCTC G[AT]GC[AT]^C 162 GTGCTC Bsp1286I 124 GTGCTC G[AGT]GC[ACT]^C 162 GTGCTC DpnII 6 GATC ^GATC 153 GATC EaeI 111 CGGCCG [CT]^GGCC[GA] 174 TGGCCG EcoNI 49 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 135 CCTTCCAAAGG HaeIII 112 GGCC GG^CC 175 GGCC HinfI 68 GAATC G^A[ACGT]TC 101 GAGTC MboI 6 GATC ^GATC 153 GATC NdeII 6 GATC ^GATC 153 GATC NlaIV 5 GGATCC GG[ACGT]^[ACGT]CC 45 GGTGCC Sau3AI 6 GATC ^GATC 153 GATC TseI 131 GCTGC G^C[AT]GC 185 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### BsiYI BslI Fnu4HI ItaI MboII MnlI