(Circular) (Six-base) MAPSORT of: pFN21AB7639.seq Check: 7617 from: 1 to: 5683 pFN21AB7639 5683 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 3026 1937 Size: 84 1005 4594 Fragments arranged by size: 4594 1005 84 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2595 3746 5130 5398 1632 Size: 963 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 963 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2694 4582 5559 1203 Size: 1491 1888 977 1327 Fragments arranged by size: 1888 1491 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 2714 1166 Size: 1548 4135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2638 1184 Size: 1454 4229 AlwNI CAG_nnn'CTG Cuts at: 2677 2827 3426 4998 2677 Size: 150 599 1572 3362 Fragments arranged by size: 3362 1572 599 150 ApaI G_GGCC'C Cuts at: 2191 2530 2191 Size: 339 5344 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2425 2992 3218 3370 5479 1493 Size: 932 567 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 567 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2184 3008 1870 Size: 73 241 824 4545 Fragments arranged by size: 4545 824 241 73 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2322 2357 2504 2810 Size: 325 217 627 378 157 35 147 306 Cuts at: 2810 2928 2964 3004 4459 618 Size: 118 36 40 1455 1842 Fragments arranged by size: 1842 1455 627 378 325 306 217 157 147 118 40 36 35 BanII G_rGCy'C Cuts at: 1391 2136 2191 2208 2530 2559 2741 2772 3002 Size: 745 55 17 322 29 182 31 230 Cuts at: 3002 1391 Size: 4072 Fragments arranged by size: 4072 745 322 230 182 55 31 29 17 BbeI G_GCGC'C Cuts at: 2169 2326 2814 2932 2968 2169 Size: 157 488 118 36 4884 Fragments arranged by size: 4884 488 157 118 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 2179 3100 2179 Size: 921 4762 Bme1580I G_kGCm'C Cuts at: 1790 2191 2530 3744 4900 1790 Size: 401 339 1214 1156 2573 Fragments arranged by size: 2573 1214 1156 401 339 BmgBI CAC'GTC Cuts at: 1318 2695 1318 Size: 1377 4306 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2490 3414 4372 Size: 416 53 83 399 24 1073 924 958 Cuts at: 4372 442 Size: 1753 Fragments arranged by size: 1753 1073 958 924 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2234 2240 2322 2347 4343 1239 Size: 911 84 6 82 25 1996 2579 Fragments arranged by size: 2579 1996 911 84 82 25 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2710 3808 4673 4971 2710 Size: 1098 865 298 3422 Fragments arranged by size: 3422 1098 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2323 2784 2811 Size: 53 83 186 936 633 157 461 27 Cuts at: 2811 2929 2965 3490 3872 4507 275 Size: 118 36 525 382 635 1451 Fragments arranged by size: 1451 936 635 633 525 461 382 186 157 118 83 53 36 27 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 1178 Size: 30 165 30 633 30 4795 Fragments arranged by size: 4795 633 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2963 1186 Size: 871 906 3906 Fragments arranged by size: 3906 906 871 BseYI C'CCAG_C Cuts at: 2037 2260 2530 2541 4886 5382 2037 Size: 223 270 11 2345 496 2338 Fragments arranged by size: 2345 2338 496 270 223 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2208 2772 3744 3829 4900 2136 Size: 72 564 972 85 1071 2919 Fragments arranged by size: 2919 1071 972 564 85 72 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2191 2208 2530 2559 2741 2772 Size: 399 346 55 17 322 29 182 31 Cuts at: 2772 3002 3744 3829 4900 1391 Size: 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 322 230 182 85 55 31 29 17 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2304 3038 844 Size: 1415 45 734 3489 Fragments arranged by size: 3489 1415 734 45 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 2490 2925 4327 4514 1700 Size: 110 515 165 435 1402 187 2869 Fragments arranged by size: 2869 1402 515 435 187 165 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 2312 1925 Size: 387 5296 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 2827 794 Size: 1478 555 3650 Fragments arranged by size: 3650 1478 555 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 3969 Fragments arranged by size: 3969 939 552 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2721 3199 3993 4013 2240 Size: 481 478 794 20 3910 Fragments arranged by size: 3910 794 481 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2327 2923 3130 Size: 54 1425 50 275 18 497 596 207 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 596 497 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2374 3613 1353 Size: 1021 1239 3423 Fragments arranged by size: 3423 1239 1021 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2494 2533 4270 4644 4790 387 Size: 1064 138 905 39 1737 374 146 1280 Fragments arranged by size: 1737 1280 1064 905 374 146 138 39 EcoICRI GAG'CTC Cuts at: 2134 2206 2770 2134 Size: 72 564 5047 Fragments arranged by size: 5047 564 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2234 2240 2322 2347 2595 3746 Size: 393 518 84 6 82 25 248 1151 Cuts at: 3746 4343 5130 5398 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1151 787 597 518 393 268 248 84 82 25 6 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2846 2858 2819 Size: 27 12 5644 Fragments arranged by size: 5644 27 12 EcoO109I rG'GnC_Cy Cuts at: 2526 2527 2731 2824 2851 2526 Size: 1 204 93 27 5358 Fragments arranged by size: 5358 204 93 27 1 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FseI GG_CCGG'CC Cuts at: 2494 2494 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2012 2169 2227 2326 2678 2814 2932 2968 Size: 334 157 58 99 352 136 118 36 Cuts at: 2968 4830 1678 Size: 1862 2531 Fragments arranged by size: 2531 1862 352 334 157 136 118 99 58 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 4324 4356 4398 Size: 32 135 32 1151 32 1569 32 42 Cuts at: 4398 4430 1373 Size: 32 2626 Fragments arranged by size: 2626 1569 1151 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2022 3027 3273 669 Size: 988 281 84 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 84 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KasI G'GCGC_C Cuts at: 2165 2322 2810 2928 2964 2165 Size: 157 488 118 36 4884 Fragments arranged by size: 4884 488 157 118 36 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2714 3641 4000 4159 518 Size: 648 1548 927 359 159 2042 Fragments arranged by size: 2042 1548 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2196 2229 2476 2689 3099 3776 4924 5169 Size: 590 33 247 213 410 677 1148 245 Cuts at: 5169 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 590 410 247 245 213 33 NaeI GCC'GGC Cuts at: 2327 2492 2927 2327 Size: 165 435 5083 Fragments arranged by size: 5083 435 165 NarI GG'CG_CC Cuts at: 2166 2323 2811 2929 2965 2166 Size: 157 488 118 36 4884 Fragments arranged by size: 4884 488 157 118 36 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2325 2490 2925 2325 Size: 165 435 5083 Fragments arranged by size: 5083 435 165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 2677 1108 Size: 1569 4114 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2731 2731 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2187 2526 2187 Size: 339 5344 PstI C_TGCA'G Cuts at: 830 2823 3035 830 Size: 1993 212 3478 Fragments arranged by size: 3478 1993 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2689 2689 Size: 5683 SacI G_AGCT'C Cuts at: 2136 2208 2772 2136 Size: 72 564 5047 Fragments arranged by size: 5047 564 72 SacII CC_GC'GG Cuts at: 2230 2230 Size: 5683 SalI G'TCGA_C Cuts at: 1936 2020 3025 1936 Size: 84 1005 4594 Fragments arranged by size: 4594 1005 84 SapI GCTCTTCn'nnn_ Cuts at: 2374 2374 Size: 5683 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 2621 3931 5643 2621 Size: 1310 1712 2661 Fragments arranged by size: 2661 1712 1310 SfcI C'TryA_G Cuts at: 826 1045 2347 2819 3031 4166 4847 5038 826 Size: 219 1302 472 212 1135 681 191 1471 Fragments arranged by size: 1471 1302 1135 681 472 219 212 191 SfoI GGC'GCC Cuts at: 2167 2324 2812 2930 2966 2167 Size: 157 488 118 36 4884 Fragments arranged by size: 4884 488 157 118 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2186 3010 1872 Size: 314 824 4545 Fragments arranged by size: 4545 824 314 SmlI C'TyrA_G Cuts at: 820 1017 1943 2725 3787 4688 4950 820 Size: 197 926 782 1062 901 262 1553 Fragments arranged by size: 1553 1062 926 901 782 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SrfI GCCC'GGGC Cuts at: 2186 2186 Size: 5683 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2978 1517 Size: 1461 4222 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 3122 513 Size: 552 939 636 482 3074 Fragments arranged by size: 3074 939 636 552 482 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 3710 3727 3880 4065 4510 325 Size: 1255 552 1578 17 153 185 445 1498 Fragments arranged by size: 1578 1498 1255 552 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 3929 5641 Size: 274 80 33 51 1443 642 1310 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1443 1310 642 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 5643 5675 2036 Size: 32 3575 32 2044 Fragments arranged by size: 3575 2044 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2184 3008 1870 Size: 314 824 4545 Fragments arranged by size: 4545 824 314 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SwaI XcmI