Restriction map for
pFN21AE0271
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I MaeI MaeIII
MamI MfeI MluI MroI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 38 ACATGT A^C[GA][CT]GT
AlwNI 706 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 927 CCTGAGG CC^T[ACGT]AGG
ApaI 421 GGGCCC GGGCC^C
AvaI 418 CTCGGG C^[CT]CG[GA]G
BamHI 135 GGATCC G^GATCC
BbeI 514 GGCGCC GGCGC^C
BsiEI 47 CGGCCG CG[GA][CT]^CG
BsoBI 418 CTCGGG C^[CT]CG[GA]G
BspLU11I 38 ACATGT A^CATGT
BsrBI 347 GAGCGG CCGCTC(-3/-3)
BsrDI 677 GCAATG GCAATG(2/0)
BssSI 785 CTCGTG CACGAG(-5/-1)
BstAPI 78 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 852 GGATG GGATG(2/0)
BstXI 293 CCAGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 135 GGATCC [GA]^GATC[CT]
Bsu36I 927 CCTGAGG CC^T[ACGT]AGG
DpnII 136 GATC ^GATC
EagI 47 CGGCCG C^GGCCG
EclXI 47 CGGCCG C^GGCCG
Eco52I 47 CGGCCG C^GGCCG
Eco81I 927 CCTGAGG CC^T[ACGT]AGG
FokI 852 GGATG GGATG(9/13)
HincII 835 GTCAAC GT[CT]^[GA]AC
HindII 835 GTCAAC GT[CT]^[GA]AC
HinfI 857 GACTC G^A[ACGT]TC
KasI 514 GGCGCC G^GCGCC
KspI 443 CCGCGG CCGC^GG
MboI 136 GATC ^GATC
MboII 1039 GAAGA GAAGA(8/7)
MflI 135 GGATCC [GA]^GATC[CT]
NaeI 175 GCCGGC GCC^GGC
NarI 514 GGCGCC GG^CGCC
NdeII 136 GATC ^GATC
NgoMIV 175 GCCGGC G^CCGGC
NspI 38 ACATGT [GA]CATG^[CT]
PleI 857 GACTC GAGTC(4/5)
PshAI 1058 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 443 CCGCGG CCGC^GG
Sau3AI 136 GATC ^GATC
SfaNI 658 GATGC GCATC(5/9)
SfoI 514 GGCGCC GGC^GCC
StuI 644 AGGCCT AGG^CCT
TaqI 999 TCGA T^CGA
TspRI 739 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 999 TCGA T^CGA
XhoII 135 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 355 GACGCC G[GA]^CG[CT]C
514 GGCGCC
AlwI 136 GATCC GGATC(4/5)
135 GGATC
AspHI 76 GAGCAC G[AT]GC[AT]^C
322 GTGCTC
BanII 421 GGGCCC G[GA]GC[CT]^C
573 GGGCTC
BglI 283 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
743 GCCATGTGGGC
BsaAI 940 TACGTG [CT]AC^GT[GA]
969 TACGTG
BsaHI 355 GACGCC G[GA]^CG[CT]C
514 GGCGCC
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
322 GTGCTC
BspMI 28 ACCTGC ACCTGC(4/8)
719 ACCTGC
BsrFI 175 GCCGGC [GA]^CCGG[CT]
247 GCCGGT
BsrI 201 CCAGT ACTGG(1/-1)
965 CCAGT
Cfr10I 175 GCCGGC [GA]^CCGG[CT]
247 GCCGGT
DdeI 859 CTCAG C^T[ACGT]AG
928 CTGAG
Hin1I 355 GACGCC G[GA]^CG[CT]C
514 GGCGCC
HphI 150 GGTGA GGTGA(8/7)
250 GGTGA
PstI 340 CTGCAG CTGCA^G
460 CTGCAG
PvuII 365 CAGCTG CAG^CTG
604 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BalI BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MaeII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SfcI StyI
TseI