Restriction map for
pFN21AE0271
(Circular) (Six-base) MAPSORT of: pFN21AE0271.seq Check: 6852 from: 1 to: 5779
pFN21AE0271 5779 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2042 2042
Size: 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2043 4678 5655 1203
Size: 840 2635 977 1327
Fragments arranged by size:
2635 1327 977 840
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2716 3522 5094 2716
Size: 806 1572 3401
Fragments arranged by size:
3401 1572 806
ApaI G_GGCC'C
Cuts at: 2430 2430
Size: 5779
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2423 3104 1870
Size: 73 480 681 4545
Fragments arranged by size:
4545 681 480 73
BamHI G'GATC_C
Cuts at: 2140 3109 3476 2140
Size: 969 367 4443
Fragments arranged by size:
4443 969 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2107 2519 3100 4555
Size: 325 217 627 243 77 412 581 1455
Cuts at: 4555 618
Size: 1842
Fragments arranged by size:
1842 1455 627 581 412 325 243 217
77
BanII G_rGCy'C
Cuts at: 1391 2430 2582 3098 1391
Size: 1039 152 516 4072
Fragments arranged by size:
4072 1039 516 152
BbeI G_GCGC'C
Cuts at: 2523 2523
Size: 5779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3970 4004 1903
Size: 34 50 34 1949 34 3678
Fragments arranged by size:
3678 1949 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2294 2754 4390
Size: 107 122 71 631 605 622 460 1636
Cuts at: 4390 136
Size: 1525
Fragments arranged by size:
1636 1525 631 622 605 460 122 107
71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2051 2430 3056 3840 4996 1790
Size: 243 18 379 626 784 1156 2573
Fragments arranged by size:
2573 1156 784 626 379 243 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2693 3038 4439 1239
Size: 1163 291 345 1401 2579
Fragments arranged by size:
2579 1401 1163 345 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2947 2976 3570 493
Size: 711 1743 29 594 2702
Fragments arranged by size:
2702 1743 711 594 29
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2361 2520 3586 3968
Size: 53 83 186 936 828 159 1066 382
Cuts at: 3968 4603 275
Size: 635 1451
Fragments arranged by size:
1451 1066 936 828 635 382 186 159
83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2930 3035 3053 1186
Size: 1744 105 18 3912
Fragments arranged by size:
3912 1744 105 18
BseYI C'CCAG_C
Cuts at: 2067 2201 3073 4982 5478 2067
Size: 134 872 1909 496 2368
Fragments arranged by size:
2368 1909 872 496 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2498 2762 1163
Size: 573 339 423 264 4180
Fragments arranged by size:
4180 573 423 339 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3229 3990 5018 1540
Size: 275 186 54 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2085 2331 3840 3925 4996 2085
Size: 246 1509 85 1071 2868
Fragments arranged by size:
2868 1509 1071 246 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2051 2085 2331 2430 2582 3056
Size: 399 243 18 34 246 99 152 474
Cuts at: 3056 3098 3840 3925 4996 1391
Size: 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 246 243 152
99 85 42 34 18
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2042 2733 3134 844
Size: 1198 691 401 3489
Fragments arranged by size:
3489 1198 691 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2354 3666 1394
Size: 429 531 1312 3507
Fragments arranged by size:
3507 1312 531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 4274 4448 65
Size: 1183 1441 1585 174 1396
Fragments arranged by size:
1585 1441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 2252 4423 4610 1700
Size: 110 370 72 2171 187 2869
Fragments arranged by size:
2869 2171 370 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 2062 2149 2626 1925
Size: 137 87 477 5078
Fragments arranged by size:
5078 477 137 87
BssSI C'ACGA_G
Cuts at: 2790 3839 4851 2790
Size: 1049 1012 3718
Fragments arranged by size:
3718 1049 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4484
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2140 3109 3476 3861 3878 5330 5416 5428
Size: 168 969 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 969 385 367 346 168 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2933 2933
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2025 2448 2893 513
Size: 552 960 423 445 3399
Fragments arranged by size:
3399 960 552 445 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 4003
Fragments arranged by size:
4003 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 2750 3295 4089 4109 2750
Size: 545 794 20 4420
Fragments arranged by size:
4420 794 545 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2232 2250
Size: 54 1425 50 275 18 222 180 18
Cuts at: 2250 2295 2394 3226 4115 5744 8
Size: 45 99 832 889 1629 43
Fragments arranged by size:
1629 1425 889 832 275 222 180 99
54 50 45 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 3226 1537
Size: 275 240 1174 4090
Fragments arranged by size:
4090 1174 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 3709 1353
Size: 2356 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2205 2279 4366 4740 4886 387
Size: 1064 138 616 74 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 616 374 146 138 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2693 3038 3842 4439 5226 5494
Size: 393 770 291 345 804 597 787 268
Cuts at: 5494 1239
Size: 1524
Fragments arranged by size:
1524 804 787 770 597 393 345 291
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2645 2885 3020 2597
Size: 48 240 135 5356
Fragments arranged by size:
5356 240 135 48
EcoO109I rG'GnC_Cy
Cuts at: 2125 2444 2554 2878 3069 2125
Size: 319 110 324 191 4835
Fragments arranged by size:
4835 324 319 191 110
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2274 2345 2393 2506 2523 4926 1678
Size: 596 71 48 113 17 2403 2531
Fragments arranged by size:
2531 2403 596 113 71 48 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2809 2841 4420 4452 4494
Size: 32 135 32 1237 32 1579 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 1579 1237 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2842 3123 3369 669
Size: 988 281 904 281 246 3079
Fragments arranged by size:
3079 988 904 281 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2519 2519
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2297 2396 10
Size: 54 1425 343 402 63 99 3393
Fragments arranged by size:
3393 1425 402 343 99 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3737 4096 4255 518
Size: 648 2571 359 159 2042
Fragments arranged by size:
2571 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2372 2450 2611 3195 3872 5020 5265 1606
Size: 766 78 161 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 584 245 161 78
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 5779
NarI GG'CG_CC
Cuts at: 2520 2520
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2047 3137 4682 1132
Size: 97 818 1090 1545 2229
Fragments arranged by size:
2229 1545 1090 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 2043 4678 2043
Size: 2635 3144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2444 2878 3069 2125
Size: 319 434 191 4835
Fragments arranged by size:
4835 434 319 191
PshAI GACnn'nnGTC
Cuts at: 1613 3067 1613
Size: 1454 4325
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2426 2426
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2349 2469 3131 830
Size: 1519 120 662 3478
Fragments arranged by size:
3478 1519 662 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2372 2611 2372
Size: 239 5540
SacII CC_GC'GG
Cuts at: 2451 2451
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SanDI GG'GwC_CC
Cuts at: 2444 3069 2444
Size: 625 5154
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2345 2465 2606 3127 4262 4943 5134
Size: 219 1300 120 141 521 1135 681 191
Cuts at: 5134 826
Size: 1471
Fragments arranged by size:
1471 1300 1135 681 521 219 191 141
120
SfoI GGC'GCC
Cuts at: 2521 2521
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2651 1517
Size: 1134 4645
StyI C'CwwG_G
Cuts at: 513 1065 2652 2808 2904 3218 513
Size: 552 1587 156 96 314 3074
Fragments arranged by size:
3074 1587 552 314 156 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 4025 5737
Size: 274 80 33 51 1443 186 1862 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1862 1712 1443 274 186 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SapI SexAI SfiI SrfI SwaI XcmI