Restriction map for
pFN21AE0271
(Linear) (Six-base) MAPSORT of: KIEE0271.seq Check: 7211 from: 1 to: 1074
KIEE0271.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 37 1074
Size: 37 1037
AflIII A'CryG_T
Cuts at: 0 38 1074
Size: 38 1036
AlwNI CAG_nnn'CTG
Cuts at: 0 711 1074
Size: 711 363
ApaI G_GGCC'C
Cuts at: 0 425 1074
Size: 425 649
AvaI C'yCGr_G
Cuts at: 0 418 1074
Size: 418 656
BamHI G'GATC_C
Cuts at: 0 135 1074
Size: 135 939
BanI G'GyrC_C
Cuts at: 0 25 102 514 1074
Size: 25 77 412 560
Fragments arranged by size:
560 412 77 25
BanII G_rGCy'C
Cuts at: 0 425 577 1074
Size: 425 152 497
Fragments arranged by size:
497 425 152
BbeI G_GCGC'C
Cuts at: 0 518 1074
Size: 518 556
BglI GCCn_nnn'nGGC
Cuts at: 0 289 749 1074
Size: 289 460 325
Fragments arranged by size:
460 325 289
Bme1580I G_kGCm'C
Cuts at: 0 28 46 425 1051 1074
Size: 28 18 379 626 23
Fragments arranged by size:
626 379 28 23 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 1074
Size: 340 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 688 1033 1074
Size: 397 291 345 41
Fragments arranged by size:
397 345 291 41
BsaAI yAC'GTr
Cuts at: 0 942 971 1074
Size: 942 29 103
Fragments arranged by size:
942 103 29
BsaHI Gr'CG_yC
Cuts at: 0 356 515 1074
Size: 356 159 559
Fragments arranged by size:
559 356 159
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 925 1030 1048 1074
Size: 925 105 18 26
Fragments arranged by size:
925 105 26 18
BseYI C'CCAG_C
Cuts at: 0 62 196 1068 1074
Size: 62 134 872 6
Fragments arranged by size:
872 134 62 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 493 757 1074
Size: 70 423 264 317
Fragments arranged by size:
423 317 264 70
BsiEI CG_ry'CG
Cuts at: 0 50 1074
Size: 50 1024
BsiHKAI G_wGCw'C
Cuts at: 0 80 326 1074
Size: 80 246 748
Fragments arranged by size:
748 246 80
Bsp1286I G_dGCh'C
Cuts at: 0 28 46 80 326 425 577 1051 1074
Size: 28 18 34 246 99 152 474 23
Fragments arranged by size:
474 246 152 99 34 28 23 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 37 728 1074
Size: 37 691 346
Fragments arranged by size:
691 346 37
BsrBI CCG'CTC
Cuts at: 0 349 1074
Size: 349 725
BsrDI GCAATG_nn'
Cuts at: 0 684 1074
Size: 684 390
BsrFI r'CCGG_y
Cuts at: 0 175 247 1074
Size: 175 72 827
Fragments arranged by size:
827 175 72
BssHII G'CGCG_C
Cuts at: 0 57 144 621 1074
Size: 57 87 477 453
Fragments arranged by size:
477 453 87 57
BssSI C'ACGA_G
Cuts at: 0 785 1074
Size: 785 289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 1074
Size: 84 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 1074
Size: 300 774
BstYI r'GATC_y
Cuts at: 0 135 1074
Size: 135 939
Bsu36I CC'TnA_GG
Cuts at: 0 928 1074
Size: 928 146
BtgI C'CryG_G
Cuts at: 0 20 443 888 1074
Size: 20 423 445 186
Fragments arranged by size:
445 423 186 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 1074
Size: 258 816
BtsI GCAGTG_nn'
Cuts at: 0 745 1074
Size: 745 329
EaeI y'GGCC_r
Cuts at: 0 47 227 245 290 389 1074
Size: 47 180 18 45 99 685
Fragments arranged by size:
685 180 99 47 45 18
EagI C'GGCC_G
Cuts at: 0 47 1074
Size: 47 1027
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 200 274 1074
Size: 200 74 800
Fragments arranged by size:
800 200 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 688 1033 1074
Size: 397 291 345 41
Fragments arranged by size:
397 345 291 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 640 880 1015 1074
Size: 592 48 240 135 59
Fragments arranged by size:
592 240 135 59 48
EcoO109I rG'GnC_Cy
Cuts at: 0 120 439 549 873 1064 1074
Size: 120 319 110 324 191 10
Fragments arranged by size:
324 319 191 120 110 10
HaeII r_GCGC'y
Cuts at: 0 269 340 388 501 518 1074
Size: 269 71 48 113 17 556
Fragments arranged by size:
556 269 113 71 48 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 804 836 1074
Size: 804 32 238
Fragments arranged by size:
804 238 32
HincII GTy'rAC
Cuts at: 0 837 1074
Size: 837 237
KasI G'GCGC_C
Cuts at: 0 514 1074
Size: 514 560
MscI TGG'CCA
Cuts at: 0 229 292 391 1074
Size: 229 63 99 683
Fragments arranged by size:
683 229 99 63
MspA1I CmG'CkG
Cuts at: 0 367 445 606 1074
Size: 367 78 161 468
Fragments arranged by size:
468 367 161 78
NaeI GCC'GGC
Cuts at: 0 177 1074
Size: 177 897
NarI GG'CG_CC
Cuts at: 0 515 1074
Size: 515 559
NgoMIV G'CCGG_C
Cuts at: 0 175 1074
Size: 175 899
NspI r_CATG'y
Cuts at: 0 42 1074
Size: 42 1032
PciI A'CATG_T
Cuts at: 0 38 1074
Size: 38 1036
PfoI T'CCnGG_A
Cuts at: 0 326 1074
Size: 326 748
PpuMI rG'GwC_Cy
Cuts at: 0 120 439 873 1064 1074
Size: 120 319 434 191 10
Fragments arranged by size:
434 319 191 120 10
PshAI GACnn'nnGTC
Cuts at: 0 1062 1074
Size: 1062 12
PspOMI G'GGCC_C
Cuts at: 0 421 1074
Size: 421 653
PstI C_TGCA'G
Cuts at: 0 344 464 1074
Size: 344 120 610
Fragments arranged by size:
610 344 120
PvuII CAG'CTG
Cuts at: 0 367 606 1074
Size: 367 239 468
Fragments arranged by size:
468 367 239
SacII CC_GC'GG
Cuts at: 0 446 1074
Size: 446 628
SanDI GG'GwC_CC
Cuts at: 0 439 1064 1074
Size: 439 625 10
Fragments arranged by size:
625 439 10
SfcI C'TryA_G
Cuts at: 0 340 460 601 1074
Size: 340 120 141 473
Fragments arranged by size:
473 340 141 120
SfoI GGC'GCC
Cuts at: 0 516 1074
Size: 516 558
StuI AGG'CCT
Cuts at: 0 646 1074
Size: 646 428
StyI C'CwwG_G
Cuts at: 0 647 803 899 1074
Size: 647 156 96 175
Fragments arranged by size:
647 175 156 96
TatI w'GTAC_w
Cuts at: 0 158 1074
Size: 158 916
Enzymes that do cut:
AarI AflIII AlwNI ApaI AvaI BamHI BanI BanII
BbeI BglI Bme1580I BplI BpmI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI MscI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PpuMI PshAI PspOMI PstI
PvuII SacII SanDI SfcI SfoI StuI StyI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI