################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BpuAI BsaAI BsaHI BsaI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI DpnII DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MboI MboII MfeI MflI MluI MroI MseI MslI MunI NaeI NarI NdeI NdeII NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 24 GAATTT [GA]^AATT[CT] AfaI 124 GTAC GT^AC ApoI 24 GAATTT [GA]^AATT[CT] BalI 137 TGGCCA TGG^CCA BanI 205 GGCACC G^G[CT][GA]CC BanII 163 GAGCCC G[GA]GC[CT]^C BciVI 284 GGATAC GTATCC(6/5) BcnI 130 CCGGG CC^[GC]GG BfaI 265 CTAG C^TAG BglI 45 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 147 GCTGAGC GC^T[ACGT]AGC Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC BsaBI 285 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 281 ACCGGA [AT]^CCGG[AT] BseRI 186 CTCCTC GAGGAG(10/8) BsiEI 127 CGGCCG CG[GA][CT]^CG BsmFI 334 GGGAC GGGAC(10/14) BspHI 341 TCATGA T^CATGA BspMI 99 ACCTGC ACCTGC(4/8) BsrBI 150 GAGCGG CCGCTC(-3/-3) BssHII 170 GCGCGC G^CGCGC BstF5I 87 CATCC GGATG(2/0) CelII 147 GCTGAGC GC^T[ACGT]AGC CpoI 104 CGGACCG CG^G[AT]CCG DdeI 148 CTGAG C^T[ACGT]AG EagI 127 CGGCCG C^GGCCG EclXI 127 CGGCCG C^GGCCG Eco52I 127 CGGCCG C^GGCCG EcoNI 270 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 87 CATCC GGATG(9/13) HphI 254 TCACC GGTGA(8/7) KspI 47 CCGCGG CCGC^GG MaeI 265 CTAG C^TAG MaeII 116 ACGT A^CGT MaeIII 118 GTGAC ^GT[ACGT]AC MamI 285 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 137 TGGCCA TGG^CCA MscI 137 TGGCCA TGG^CCA NciI 130 CCGGG CC^[GC]GG NcoI 140 CCATGG C^CATGG NheI 264 GCTAGC G^CTAGC PvuII 175 CAGCTG CAG^CTG RcaI 341 TCATGA T^CATGA RsaI 124 GTAC GT^AC RsrII 104 CGGACCG CG^G[AT]CCG SacII 47 CCGCGG CCGC^GG Tsp45I 118 GTGAC ^GT[GC]AC Tsp509I 25 AATT ^AATT XcmI 191 CCATCGGCGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BpmI 10 CTGGAG CTGGAG(16/14) 55 CTGGAG DsaI 47 CCGCGG C^C[GA][CT]GG 140 CCATGG EaeI 127 CGGCCG [CT]^GGCC[GA] 137 TGGCCA HaeII 144 GGCGCT [GA]GCGC^[CT] 232 AGCGCC HapII 130 CCGG C^CGG 282 CCGG HinfI 65 GACTC G^A[ACGT]TC 310 GAGTC HpaII 130 CCGG C^CGG 282 CCGG MspI 130 CCGG C^CGG 282 CCGG NlaIII 141 CATG CATG^ 342 CATG NlaIV 154 GGGTCC GG[ACGT]^[ACGT]CC 205 GGCACC PleI 65 GACTC GAGTC(4/5) 310 GAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII EcoT14I FauI Fnu4HI HaeIII HhaI HinP1I ItaI MnlI MspA1I MvaI MvnI MwoI Sau96I ScrFI StyI TseI