(Circular) (Six-base) MAPSORT of: pFN21AE5127.seq Check: 5511 from: 1 to: 5059 pFN21AE5127 5059 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2869 278 Size: 53 83 186 936 1333 2468 Fragments arranged by size: 2468 1333 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2402 1937 Size: 465 4594 Acc65I G'GTAC_C Cuts at: 2380 2380 Size: 5059 AclI AA'CG_TT Cuts at: 1563 3186 3559 1563 Size: 1623 373 3063 Fragments arranged by size: 3063 1623 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3122 4506 4774 1632 Size: 1490 1384 268 1917 Fragments arranged by size: 1917 1490 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4862 AflIII A'CryG_T Cuts at: 1203 3958 4935 1203 Size: 2755 977 1327 Fragments arranged by size: 2755 1327 977 AgeI A'CCGG_T Cuts at: 3890 3890 Size: 5059 AhdI GACnn_n'nnGTC Cuts at: 3788 3788 Size: 5059 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5059 AlwNI CAG_nnn'CTG Cuts at: 2802 4374 2802 Size: 1572 3487 ApaLI G'TGCA_C Cuts at: 3116 4272 3116 Size: 1156 3903 ApoI r'AATT_y Cuts at: 1493 2029 2368 2594 2746 4855 1493 Size: 536 339 226 152 2109 1697 Fragments arranged by size: 2109 1697 536 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 3613 160 Size: 872 2581 1606 Fragments arranged by size: 2581 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5059 AvaI C'yCGr_G Cuts at: 1870 1943 2384 1870 Size: 73 441 4545 Fragments arranged by size: 4545 441 73 BamHI G'GATC_C Cuts at: 2389 2756 2389 Size: 367 4692 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2210 2380 3835 618 Size: 325 217 627 423 170 1455 1842 Fragments arranged by size: 1842 1455 627 423 325 217 170 BanII G_rGCy'C Cuts at: 1391 2172 2378 1391 Size: 781 206 4072 Fragments arranged by size: 4072 781 206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3250 3284 1903 Size: 34 50 34 1229 34 3678 Fragments arranged by size: 3678 1229 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2283 2951 4161 2283 Size: 668 1210 3181 Fragments arranged by size: 3181 1210 668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5059 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2056 3670 136 Size: 107 122 71 631 605 384 1614 1525 Fragments arranged by size: 1614 1525 631 605 384 122 107 71 BglII A'GATC_T Cuts at: 5054 5054 Size: 5059 BlpI GC'TnA_GC Cuts at: 2153 2476 2153 Size: 323 4736 Bme1580I G_kGCm'C Cuts at: 1790 2052 2213 3120 4276 1790 Size: 262 161 907 1156 2573 Fragments arranged by size: 2573 1156 907 262 161 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5059 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2790 3748 442 Size: 416 53 83 399 24 1373 958 1753 Fragments arranged by size: 1753 1373 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2273 1056 Size: 1217 3842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2081 3719 1239 Size: 797 45 1638 2579 Fragments arranged by size: 2579 1638 797 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3184 4049 4347 3184 Size: 865 298 3896 Fragments arranged by size: 3896 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3722 882 Size: 617 2223 2219 Fragments arranged by size: 2223 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2850 493 Size: 711 1646 2702 Fragments arranged by size: 2702 1646 711 BsaBI GATnn'nnATC Cuts at: 2294 5053 2294 Size: 2759 2300 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2866 3248 3883 275 Size: 53 83 186 936 1333 382 635 1451 Fragments arranged by size: 1451 1333 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2286 3492 3890 4164 4311 4722 2286 Size: 1206 398 274 147 411 2623 Fragments arranged by size: 2623 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4834 Fragments arranged by size: 4834 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 1186 Size: 996 4063 BseYI C'CCAG_C Cuts at: 4262 4758 4262 Size: 496 4563 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4486 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2509 3270 4298 1540 Size: 275 186 134 374 761 1028 2301 Fragments arranged by size: 2301 1028 761 374 275 186 134 BsiHKAI G_wGCw'C Cuts at: 3120 3205 4276 3120 Size: 85 1071 3903 Fragments arranged by size: 3903 1071 85 BsmI GAATG_Cn' Cuts at: 2570 2663 2570 Size: 93 4966 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2172 2213 2378 3120 3205 4276 Size: 399 262 120 41 165 742 85 1071 Cuts at: 4276 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 262 165 120 85 41 BspEI T'CCGG_A Cuts at: 4722 4722 Size: 5059 BspHI T'CATG_A Cuts at: 2346 2948 4678 2346 Size: 602 1730 2727 Fragments arranged by size: 2727 1730 602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2113 2414 844 Size: 1269 301 3489 Fragments arranged by size: 3489 1269 301 BsrBI CCG'CTC Cuts at: 1394 1823 2157 2946 1394 Size: 429 334 789 3507 Fragments arranged by size: 3507 789 429 334 BsrDI GCAATG_nn' Cuts at: 65 1248 3554 3728 65 Size: 1183 2306 174 1396 Fragments arranged by size: 2306 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3703 3890 1700 Size: 110 1893 187 2869 Fragments arranged by size: 2869 1893 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5059 BssHII G'CGCG_C Cuts at: 1925 2175 1925 Size: 250 4809 BssSI C'ACGA_G Cuts at: 3119 4131 3119 Size: 1012 4047 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5059 BstBI TT'CG_AA Cuts at: 3863 3863 Size: 5059 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5059 BstYI r'GATC_y Cuts at: 1972 2389 2756 3141 3158 4610 4696 4708 5054 Size: 417 367 385 17 1452 86 12 346 Cuts at: 5054 1972 Size: 1977 Fragments arranged by size: 1977 1452 417 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 2145 513 Size: 552 987 93 3427 Fragments arranged by size: 3427 987 552 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4320 Fragments arranged by size: 4320 683 56 BtsI GCAGTG_nn' Cuts at: 2575 3369 3389 2575 Size: 794 20 4245 Fragments arranged by size: 4245 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5059 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5024 DraI TTT'AAA Cuts at: 2363 2363 Size: 5059 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4066 809 Size: 507 2750 1802 Fragments arranged by size: 2750 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 2142 2506 Size: 54 1425 50 275 18 302 10 364 Cuts at: 2506 3395 5024 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 364 302 275 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2132 2506 1537 Size: 275 320 374 4090 Fragments arranged by size: 4090 374 320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2989 1353 Size: 1636 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3646 4020 4166 387 Size: 1064 138 2057 374 146 1280 Fragments arranged by size: 2057 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2081 3122 3719 4506 4774 1239 Size: 393 404 45 1041 597 787 268 1524 Fragments arranged by size: 1524 1041 787 597 404 393 268 45 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2279 Size: 5059 EcoRI G'AATT_C Cuts at: 2368 2746 2368 Size: 378 4681 FspI TGC'GCA Cuts at: 3565 3565 Size: 5059 HaeII r_GCGC'y Cuts at: 1678 2153 2241 4206 1678 Size: 475 88 1965 2531 Fragments arranged by size: 2531 1965 475 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3700 3732 3774 3806 1373 Size: 32 135 32 2128 32 42 32 2626 Fragments arranged by size: 2626 2128 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2403 2649 669 Size: 988 281 465 246 3079 Fragments arranged by size: 3079 988 465 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5059 HpaI GTT'AAC Cuts at: 2649 2649 Size: 5059 KpnI G_GTAC'C Cuts at: 2384 2384 Size: 5059 MfeI C'AATT_G Cuts at: 2658 2658 Size: 5059 MluI A'CGCG_T Cuts at: 4935 4935 Size: 5059 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4173 4357 1305 Size: 2868 184 2007 Fragments arranged by size: 2868 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 2925 Fragments arranged by size: 2925 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3376 3535 518 Size: 648 1851 359 159 2042 Fragments arranged by size: 2042 1851 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2084 2182 2475 3152 4300 4545 1606 Size: 448 30 98 293 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 448 293 245 98 30 NcoI C'CATG_G Cuts at: 513 1065 2145 513 Size: 552 1080 3427 Fragments arranged by size: 3427 1080 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5059 NheI G'CTAG_C Cuts at: 1052 2269 1052 Size: 1217 3842 NotI GC'GGCC_GC Cuts at: 2506 2506 Size: 5059 NspI r_CATG'y Cuts at: 1132 1229 2417 3962 1132 Size: 97 1188 1545 2229 Fragments arranged by size: 2229 1545 1188 97 PasI CC'CwG_GG Cuts at: 1348 2275 1348 Size: 927 4132 PciI A'CATG_T Cuts at: 3958 3958 Size: 5059 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5059 PfoI T'CCnGG_A Cuts at: 2013 2013 Size: 5059 PmeI GTTT'AAAC Cuts at: 2363 2363 Size: 5059 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3128 3160 4641 4673 3128 Size: 32 1481 32 3514 Fragments arranged by size: 3514 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5059 PsiI TTA'TAA Cuts at: 2629 2629 Size: 5059 PstI C_TGCA'G Cuts at: 830 2411 830 Size: 1581 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5059 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5059 RsrII CG'GwC_CG Cuts at: 2110 2110 Size: 5059 SacII CC_GC'GG Cuts at: 2055 2055 Size: 5059 SalI G'TCGA_C Cuts at: 1936 2401 1936 Size: 465 4594 SbfI CC_TGCA'GG Cuts at: 2411 2411 Size: 5059 ScaI AGT'ACT Cuts at: 3307 5019 3307 Size: 1712 3347 SfcI C'TryA_G Cuts at: 826 1045 2407 3542 4223 4414 826 Size: 219 1362 1135 681 191 1471 Fragments arranged by size: 1471 1362 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5059 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5059 SmaI CCC'GGG Cuts at: 1872 2386 1872 Size: 514 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3163 4064 4326 820 Size: 197 926 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2850 493 Size: 2357 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5059 SphI G_CATG'C Cuts at: 1132 2417 1132 Size: 1285 3774 SspI AAT'ATT Cuts at: 5 52 2983 5 Size: 47 2931 2081 Fragments arranged by size: 2931 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5059 StyI C'CwwG_G Cuts at: 513 1065 2145 2262 2310 2498 513 Size: 552 1080 117 48 188 3074 Fragments arranged by size: 3074 1080 552 188 117 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3103 3256 3441 3886 325 Size: 1255 1506 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3305 5017 96 Size: 274 80 33 51 1443 1328 1712 138 Fragments arranged by size: 1712 1443 1328 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5019 5051 5019 Size: 32 5027 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5059 XbaI T'CTAG_A Cuts at: 2395 2395 Size: 5059 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2203 2011 Size: 192 4867 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5059 XmaI C'CCGG_G Cuts at: 1870 2384 1870 Size: 514 4545 XmnI GAAnn'nnTTC Cuts at: 3188 3188 Size: 5059 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2867 276 Size: 53 83 186 936 1333 2468 Fragments arranged by size: 2468 1333 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI