(Linear) (Six-base) MAPSORT of: KIEE5127.seq Check: 5729 from: 1 to: 354 KIEE5127.orf With 182 enzymes: SgfI * January 29, 2014 11:47 .. ApoI r'AATT_y Cuts at: 0 24 354 Size: 24 330 BanI G'GyrC_C Cuts at: 0 205 354 Size: 205 149 BanII G_rGCy'C Cuts at: 0 167 354 Size: 167 187 BciVI GTATCCnnnnn_n' Cuts at: 0 278 354 Size: 278 76 BglI GCCn_nnn'nGGC Cuts at: 0 51 354 Size: 51 303 BlpI GC'TnA_GC Cuts at: 0 148 354 Size: 148 206 Bme1580I G_kGCm'C Cuts at: 0 47 208 354 Size: 47 161 146 Fragments arranged by size: 161 146 47 BmtI G_CTAG'C Cuts at: 0 268 354 Size: 268 86 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 76 354 Size: 31 45 278 Fragments arranged by size: 278 45 31 BsaBI GATnn'nnATC Cuts at: 0 289 354 Size: 289 65 BsaWI w'CCGG_w Cuts at: 0 281 354 Size: 281 73 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 177 354 Size: 177 177 BsiEI CG_ry'CG Cuts at: 0 130 354 Size: 130 224 Bsp1286I G_dGCh'C Cuts at: 0 47 167 208 354 Size: 47 120 41 146 Fragments arranged by size: 146 120 47 41 BspHI T'CATG_A Cuts at: 0 341 354 Size: 341 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 108 354 Size: 108 246 BsrBI CCG'CTC Cuts at: 0 152 354 Size: 152 202 BssHII G'CGCG_C Cuts at: 0 170 354 Size: 170 184 BtgI C'CryG_G Cuts at: 0 47 140 354 Size: 47 93 214 Fragments arranged by size: 214 93 47 EaeI y'GGCC_r Cuts at: 0 127 137 354 Size: 127 10 217 Fragments arranged by size: 217 127 10 EagI C'GGCC_G Cuts at: 0 127 354 Size: 127 227 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 76 354 Size: 31 45 278 Fragments arranged by size: 278 45 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 274 354 Size: 274 80 HaeII r_GCGC'y Cuts at: 0 148 236 354 Size: 148 88 118 Fragments arranged by size: 148 118 88 MscI TGG'CCA Cuts at: 0 139 354 Size: 139 215 MspA1I CmG'CkG Cuts at: 0 49 79 177 354 Size: 49 30 98 177 Fragments arranged by size: 177 98 49 30 NcoI C'CATG_G Cuts at: 0 140 354 Size: 140 214 NheI G'CTAG_C Cuts at: 0 264 354 Size: 264 90 PasI CC'CwG_GG Cuts at: 0 270 354 Size: 270 84 PfoI T'CCnGG_A Cuts at: 0 8 354 Size: 8 346 PvuII CAG'CTG Cuts at: 0 177 354 Size: 177 177 RsrII CG'GwC_CG Cuts at: 0 105 354 Size: 105 249 SacII CC_GC'GG Cuts at: 0 50 354 Size: 50 304 StyI C'CwwG_G Cuts at: 0 140 257 305 354 Size: 140 117 48 49 Fragments arranged by size: 140 117 49 48 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 198 354 Size: 198 156 Enzymes that do cut: ApoI BanI BanII BciVI BglI BlpI Bme1580I BmtI BpmI BsaBI BsaWI BseRI BsiEI Bsp1286I BspHI BspMI BsrBI BssHII BtgI EaeI EagI Eco57MI EcoNI HaeII MscI MspA1I NcoI NheI PasI PfoI PvuII RsrII SacII StyI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglII BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI