Restriction map for
pFN21AA0749
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# NO CUT ENZYMES ( 100 enzymes) #
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Acc65I AccIII AclI AcsI AfaI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AseI AsnI Asp718I AviII
AvrII BalI BclI BfrI BlnI
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 676 GACGTC GACGT^C
AccI 260 GTCGAC GT^[AC][GT]AC
AhdI 388 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1553 CCTTAGG CC^T[ACGT]AGG
AscI 1619 GGCGCGCC GG^CGCGCC
Asp700I 1826 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 388 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1870 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1851 GAAGAC GAAGAC(2/6)
BcgI 767 GCAAGGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1688 GTATCC GTATCC(6/5)
BfaI 1888 CTAG C^TAG
BglII 974 AGATCT A^GATCT
BlpI 1737 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1737 GCTCAGC GC^T[ACGT]AGC
BpuAI 1851 GAAGAC GAAGAC(2/6)
BsgI 1261 GTGCAG GTGCAG(16/14)
BssHII 1620 GCGCGC G^CGCGC
BstAPI 1263 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1553 CCTTAGG CC^T[ACGT]AGG
CelII 1737 GCTCAGC GC^T[ACGT]AGC
Eam1105I 388 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1553 CCTTAGG CC^T[ACGT]AGG
HincII 260 GTCGAC GT[CT]^[GA]AC
HindII 260 GTCGAC GT[CT]^[GA]AC
KspI 1416 CCGCGG CCGC^GG
MaeI 1888 CTAG C^TAG
MaeIII 1282 GTGAC ^GT[ACGT]AC
MluI 1287 ACGCGT A^CGCGT
PaeR7I 948 CTCGAG C^TCGAG
PflFI 1870 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 109 CTGCAG CTGCA^G
SacII 1416 CCGCGG CCGC^GG
SalI 260 GTCGAC G^TCGAC
Tsp45I 1282 GTGAC ^GT[GC]AC
Tth111I 1870 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 948 CTCGAG C^TCGAG
XmnI 1826 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 46 ACGTGT A^C[GA][CT]GT
1287 ACGCGT
BbeI 342 GGCGCC GGCGC^C
1786 GGCGCC
BbrPI 45 CACGTG CAC^GTG
1209 CACGTG
BbvCI 1429 GCTGAGG CCTCAGC(-5/-2)
1729 GCTGAGG
BsaWI 1066 ACCGGA [AT]^CCGG[AT]
1107 ACCGGA
EcoNI 141 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1812 CCTGCCCGAGG
EcoT14I 203 CCAAGG C^C[AT][AT]GG
554 CCTTGG
KasI 342 GGCGCC G^GCGCC
1786 GGCGCC
NaeI 86 GCCGGC GCC^GGC
1624 GCCGGC
NarI 342 GGCGCC GG^CGCC
1786 GGCGCC
NgoMIV 86 GCCGGC G^CCGGC
1624 GCCGGC
PmaCI 45 CACGTG CAC^GTG
1209 CACGTG
PmlI 45 CACGTG CAC^GTG
1209 CACGTG
PvuII 301 CAGCTG CAG^CTG
608 CAGCTG
SacI 155 GAGCTC GAGCT^C
1735 GAGCTC
SfaNI 912 GCATC GCATC(5/9)
1478 GCATC
SfcI 109 CTGCAG C^T[GA][CT]AG
848 CTACAG
SfoI 342 GGCGCC GGC^GCC
1786 GGCGCC
SmlI 940 CTCAAG C^T[CT][GA]AG
948 CTCGAG
StyI 203 CCAAGG C^C[AT][AT]GG
554 CCTTGG
XcmI 391 CCAGGGTCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
487 CCACGACGCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PflMI PleI
PpuMI Sau3AI Sau96I ScrFI SmaI
TaqI TfiI TseI TspRI TthHB8I
Van91I XhoII XmaCI XmaI