(Circular) (Six-base) MAPSORT of: pFN21AA0749.seq Check: 1769 from: 1 to: 6676 pFN21AA0749 6676 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2469 3253 2469 Size: 784 5892 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2685 4486 278 Size: 53 83 186 936 1149 1801 2468 Fragments arranged by size: 2468 1801 1149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2266 4019 1937 Size: 329 1753 4594 Fragments arranged by size: 4594 1753 329 Acc65I G'GTAC_C Cuts at: 3997 3997 Size: 6676 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4739 6123 6391 1632 Size: 3107 1384 268 1917 Fragments arranged by size: 3107 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2051 3292 5575 6552 1203 Size: 848 1241 2283 977 1327 Fragments arranged by size: 2283 1327 1241 977 848 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 2398 5405 2398 Size: 3007 3669 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AlwNI CAG_nnn'CTG Cuts at: 2025 3054 3178 3713 4419 5991 2025 Size: 1029 124 535 706 1572 2710 Fragments arranged by size: 2710 1572 1029 706 535 124 ApaI G_GGCC'C Cuts at: 2145 2248 2418 2442 3660 3673 2145 Size: 103 170 24 1218 13 5148 Fragments arranged by size: 5148 1218 170 103 24 13 ApaLI G'TGCA_C Cuts at: 4733 5889 4733 Size: 1156 5520 ApoI r'AATT_y Cuts at: 1493 3985 4211 4363 6472 1493 Size: 2492 226 152 2109 1697 Fragments arranged by size: 2492 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3625 3625 Size: 6676 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2163 2260 2295 2435 2579 2687 Size: 73 122 98 97 35 140 144 108 Cuts at: 2687 2953 3787 3821 4001 1870 Size: 266 834 34 180 4545 Fragments arranged by size: 4545 834 266 180 144 140 122 108 98 97 73 35 34 BamHI G'GATC_C Cuts at: 2069 2662 2729 4006 4373 2069 Size: 593 67 1277 367 4372 Fragments arranged by size: 4372 1277 593 367 67 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2347 2593 2867 2937 3068 Size: 325 217 627 560 246 274 70 131 Cuts at: 3068 3791 3997 5452 618 Size: 723 206 1455 1842 Fragments arranged by size: 1842 1455 723 627 560 325 274 246 217 206 131 70 BanII G_rGCy'C Cuts at: 1391 2032 2145 2164 2248 2258 2283 2319 2418 Size: 641 113 19 84 10 25 36 99 Cuts at: 2418 2442 2769 2954 3061 3495 3583 3660 3673 Size: 24 327 185 107 434 88 77 13 Cuts at: 3673 3744 3995 1391 Size: 71 251 4072 Fragments arranged by size: 4072 641 434 327 251 185 113 107 99 88 84 77 71 36 25 24 19 13 10 BbeI G_GCGC'C Cuts at: 2351 3795 2351 Size: 1444 5232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3863 928 Size: 971 1964 3741 Fragments arranged by size: 3741 1964 971 BbvCI CC'TCA_GC Cuts at: 3435 3735 3435 Size: 300 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2761 2795 4867 4901 1903 Size: 34 824 34 2072 34 3678 Fragments arranged by size: 3678 2072 824 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4568 5778 3704 Size: 864 1210 4602 Fragments arranged by size: 4602 1210 864 BclI T'GATC_A Cuts at: 1548 1548 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2092 2309 2356 Size: 107 122 71 631 605 420 217 47 Cuts at: 2356 3426 5287 136 Size: 1070 1861 1525 Fragments arranged by size: 1861 1525 1070 631 605 420 217 122 107 71 47 BglII A'GATC_T Cuts at: 2979 6671 2979 Size: 3692 2984 BlpI GC'TnA_GC Cuts at: 3743 4093 3743 Size: 350 6326 Bme1580I G_kGCm'C Cuts at: 1790 2145 2248 2418 2442 2598 2914 3151 3660 Size: 355 103 170 24 156 316 237 509 Cuts at: 3660 3673 4737 5893 1790 Size: 13 1064 1156 2573 Fragments arranged by size: 2573 1156 1064 509 355 316 237 170 156 103 24 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 4407 5365 Size: 416 53 83 399 24 618 2372 958 Cuts at: 5365 442 Size: 1753 Fragments arranged by size: 2372 1753 958 618 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3102 3102 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2607 3143 3485 5336 1239 Size: 850 518 536 342 1851 2579 Fragments arranged by size: 2579 1851 850 536 518 342 Bpu10I CC'TnA_GC Cuts at: 3435 3735 3435 Size: 300 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2930 4801 5666 5964 2930 Size: 1871 865 298 3642 Fragments arranged by size: 3642 1871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 2705 2723 3110 5339 882 Size: 617 708 498 18 387 2229 2219 Fragments arranged by size: 2229 2219 708 617 498 387 18 BsaAI yAC'GTr Cuts at: 493 1204 2052 2524 3216 4467 493 Size: 711 848 472 692 1251 2702 Fragments arranged by size: 2702 1251 848 711 692 472 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2189 2348 2497 2682 Size: 53 83 186 936 656 159 149 185 Cuts at: 2682 3195 3210 3522 3792 4483 4865 5500 275 Size: 513 15 312 270 691 382 635 1451 Fragments arranged by size: 1451 936 691 656 635 513 382 312 270 186 185 159 149 83 53 15 BsaWI w'CCGG_w Cuts at: 3071 3112 5109 5507 5781 5928 6339 3071 Size: 41 1997 398 274 147 411 3408 Fragments arranged by size: 3408 1997 411 398 274 147 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2887 2917 1178 Size: 30 165 30 1484 30 4937 Fragments arranged by size: 4937 1484 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3350 3482 3751 3752 3929 1186 Size: 2164 132 269 1 177 3933 Fragments arranged by size: 3933 2164 269 177 132 1 BseYI C'CCAG_C Cuts at: 2304 2502 2611 3258 3311 3565 3639 5879 6375 Size: 198 109 647 53 254 74 2240 496 Cuts at: 6375 2304 Size: 2605 Fragments arranged by size: 2605 2240 647 496 254 198 109 74 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 1163 Size: 573 1551 4552 Fragments arranged by size: 4552 1551 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2336 2513 3634 4126 4887 5915 Size: 275 186 335 177 1121 492 761 1028 Cuts at: 5915 1540 Size: 2301 Fragments arranged by size: 2301 1121 1028 761 492 335 275 186 177 BsiHKAI G_wGCw'C Cuts at: 2164 3552 3744 4737 4822 5893 2164 Size: 1388 192 993 85 1071 2947 Fragments arranged by size: 2947 1388 1071 993 192 85 BsmI GAATG_Cn' Cuts at: 4187 4280 4187 Size: 93 6583 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2145 2164 2248 2258 2283 2319 Size: 399 242 113 19 84 10 25 36 Cuts at: 2319 2418 2442 2598 2769 2914 2954 3061 3151 Size: 99 24 156 171 145 40 107 90 Cuts at: 3151 3495 3552 3583 3660 3673 3744 3995 4737 Size: 344 57 31 77 13 71 251 742 Cuts at: 4737 4822 5893 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 251 242 171 156 145 113 107 99 90 85 84 77 71 57 40 36 31 25 24 19 13 10 BspEI T'CCGG_A Cuts at: 6339 6339 Size: 6676 BspHI T'CATG_A Cuts at: 4565 6295 4565 Size: 1730 4946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2469 3253 3522 3964 4031 844 Size: 1625 784 269 442 67 3489 Fragments arranged by size: 3489 1625 784 442 269 67 BsrBI CCG'CTC Cuts at: 1394 1823 2200 2747 2924 4563 1394 Size: 429 377 547 177 1639 3507 Fragments arranged by size: 3507 1639 547 429 377 177 BsrDI GCAATG_nn' Cuts at: 65 1248 5171 5345 65 Size: 1183 3923 174 1396 Fragments arranged by size: 3923 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2508 3629 5320 5507 1700 Size: 110 281 417 1121 1691 187 2869 Fragments arranged by size: 2869 1691 1121 417 281 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 3625 1925 Size: 1700 4976 BssSI C'ACGA_G Cuts at: 4736 5748 4736 Size: 1012 5664 BstAPI GCAn_nnn'nTGC Cuts at: 794 3274 794 Size: 2480 4196 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstYI r'GATC_y Cuts at: 1972 2069 2662 2670 2729 2930 2979 3888 4006 Size: 97 593 8 59 201 49 909 118 Cuts at: 4006 4373 4758 4775 6227 6313 6325 6671 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 909 593 385 367 346 201 118 97 86 59 49 17 12 8 Bsu36I CC'TnA_GG Cuts at: 3559 3559 Size: 6676 BtgI C'CryG_G Cuts at: 513 1065 2004 3184 3351 3421 513 Size: 552 939 1180 167 70 3768 Fragments arranged by size: 3768 1180 939 552 167 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5937 Fragments arranged by size: 5937 683 56 BtsI GCAGTG_nn' Cuts at: 4192 4986 5006 4192 Size: 794 20 5862 Fragments arranged by size: 5862 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2221 2256 529 Size: 35 1657 35 4949 Fragments arranged by size: 4949 1657 35 35 DraI TTT'AAA Cuts at: 3980 3980 Size: 6676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5683 809 Size: 507 4367 1802 Fragments arranged by size: 4367 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2333 2510 Size: 54 1425 50 275 18 318 185 177 Cuts at: 2510 2748 3631 4123 5012 6641 8 Size: 238 883 492 889 1629 43 Fragments arranged by size: 1629 1425 889 883 492 318 275 238 185 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2333 2510 3631 4123 1537 Size: 275 521 177 1121 492 4090 Fragments arranged by size: 4090 1121 521 492 275 177 EarI CTCTTCn'nnn_ Cuts at: 1353 2648 3929 3962 4606 1353 Size: 1295 1281 33 644 3423 Fragments arranged by size: 3423 1295 1281 644 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3686 3817 5263 5637 5783 387 Size: 1064 138 2097 131 1446 374 146 1280 Fragments arranged by size: 2097 1446 1280 1064 374 146 138 131 EcoICRI GAG'CTC Cuts at: 2162 3742 2162 Size: 1580 5096 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2607 3143 3485 4739 5336 6123 Size: 393 457 518 536 342 1254 597 787 Cuts at: 6123 6391 1239 Size: 268 1524 Fragments arranged by size: 1524 1254 787 597 536 518 457 393 342 268 EcoNI CCTnn'n_nnAGG Cuts at: 2150 3821 2150 Size: 1671 5005 EcoO109I rG'GnC_Cy Cuts at: 2141 2142 2244 2414 2415 2439 2546 2556 2576 Size: 1 102 170 1 24 107 10 20 Cuts at: 2576 3175 3656 3657 3669 3670 3813 3919 3948 Size: 599 481 1 12 1 143 106 29 Cuts at: 3948 2141 Size: 4869 Fragments arranged by size: 4869 599 481 170 143 107 106 102 29 24 20 12 10 1 1 1 1 EcoRI G'AATT_C Cuts at: 3985 4363 3985 Size: 378 6298 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 2344 2351 2366 3795 5823 1678 Size: 666 7 15 1429 2028 2531 Fragments arranged by size: 2531 2028 1429 666 15 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2647 2679 2964 Size: 32 135 32 812 32 231 32 285 Cuts at: 2964 2996 5317 5349 5391 5423 1373 Size: 32 2321 32 42 32 2626 Fragments arranged by size: 2626 2321 812 285 231 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2267 4020 4266 669 Size: 988 281 329 1753 246 3079 Fragments arranged by size: 3079 1753 988 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6676 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KasI G'GCGC_C Cuts at: 2347 3791 2347 Size: 1444 5232 KpnI G_GTAC'C Cuts at: 4001 4001 Size: 6676 MfeI C'AATT_G Cuts at: 4275 4275 Size: 6676 MluI A'CGCG_T Cuts at: 3292 6552 3292 Size: 3260 3416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2728 3110 3269 3582 3808 5790 5974 1305 Size: 1423 382 159 313 226 1982 184 2007 Fragments arranged by size: 2007 1982 1423 382 313 226 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4854 Fragments arranged by size: 4854 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4634 4993 5152 518 Size: 648 3468 359 159 2042 Fragments arranged by size: 3468 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2502 2615 3423 3622 3863 4092 4769 Size: 702 194 113 808 199 241 229 677 Cuts at: 4769 5917 6162 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 808 702 677 245 241 229 199 194 113 NaeI GCC'GGC Cuts at: 2093 3631 2093 Size: 1538 5138 NarI GG'CG_CC Cuts at: 2348 3792 2348 Size: 1444 5232 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5185 Fragments arranged by size: 5185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NgoMIV G'CCGG_C Cuts at: 2091 3629 2091 Size: 1538 5138 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 4034 5579 1132 Size: 97 2805 1545 2229 Fragments arranged by size: 2805 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2097 2396 2418 3054 3172 3583 3653 1348 Size: 749 299 22 636 118 411 70 4371 Fragments arranged by size: 4371 749 636 411 299 118 70 22 PciI A'CATG_T Cuts at: 5575 5575 Size: 6676 PflMI CCAn_nnn'nTGG Cuts at: 1108 2025 2741 2997 3054 1108 Size: 917 716 256 57 4730 Fragments arranged by size: 4730 917 716 256 57 PfoI T'CCnGG_A Cuts at: 3061 3061 Size: 6676 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PmlI CAC'GTG Cuts at: 2052 3216 2052 Size: 1164 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 6258 6290 4745 Size: 32 1481 32 5131 Fragments arranged by size: 5131 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2556 3813 3919 3948 2556 Size: 1257 106 29 5284 Fragments arranged by size: 5284 1257 106 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6676 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PspOMI G'GGCC_C Cuts at: 2141 2244 2414 2438 3656 3669 2141 Size: 103 170 24 1218 13 5148 Fragments arranged by size: 5148 1218 170 103 24 13 PspXI vC'TCGA_Gb Cuts at: 2953 2953 Size: 6676 PstI C_TGCA'G Cuts at: 830 2118 4028 830 Size: 1288 1910 3478 Fragments arranged by size: 3478 1910 1288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 PvuII CAG'CTG Cuts at: 2308 2615 2308 Size: 307 6369 SacI G_AGCT'C Cuts at: 2164 3744 2164 Size: 1580 5096 SacII CC_GC'GG Cuts at: 3424 3424 Size: 6676 SalI G'TCGA_C Cuts at: 1936 2265 4018 1936 Size: 329 1753 4594 Fragments arranged by size: 4594 1753 329 SanDI GG'GwC_CC Cuts at: 3948 3948 Size: 6676 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 4924 6636 4924 Size: 1712 4964 SfcI C'TryA_G Cuts at: 826 1045 2114 2853 4024 5159 5840 6031 826 Size: 219 1069 739 1171 1135 681 191 1471 Fragments arranged by size: 1471 1171 1135 1069 739 681 219 191 SfoI GGC'GCC Cuts at: 2349 3793 2349 Size: 1444 5232 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 2067 2262 2437 2581 3789 4003 1872 Size: 195 195 175 144 1208 214 4545 Fragments arranged by size: 4545 1208 214 195 195 175 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 2945 2953 4780 5681 5943 820 Size: 197 926 1002 8 1827 901 262 1553 Fragments arranged by size: 1827 1553 1002 926 901 262 197 8 SnaBI TAC'GTA Cuts at: 493 4467 493 Size: 3974 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6676 SphI G_CATG'C Cuts at: 1132 4034 1132 Size: 2902 3774 SrfI GCCC'GGGC Cuts at: 2437 2437 Size: 6676 SspI AAT'ATT Cuts at: 5 52 4600 5 Size: 47 4548 2081 Fragments arranged by size: 4548 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2004 2208 2559 4115 513 Size: 552 939 204 351 1556 3074 Fragments arranged by size: 3074 1556 939 552 351 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 4703 4720 4873 5058 5503 325 Size: 1255 651 2472 17 153 185 445 1498 Fragments arranged by size: 2472 1498 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4922 6634 96 Size: 274 80 33 51 1443 2945 1712 138 Fragments arranged by size: 2945 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3343 3375 6636 6668 3343 Size: 32 3261 32 3351 Fragments arranged by size: 3351 3261 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3878 1316 Size: 2562 4114 XbaI T'CTAG_A Cuts at: 4012 4012 Size: 6676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2403 2499 2403 Size: 96 6580 XhoI C'TCGA_G Cuts at: 1943 2953 1943 Size: 1010 5666 XmaI C'CCGG_G Cuts at: 1870 2065 2260 2435 2579 3787 4001 1870 Size: 195 195 175 144 1208 214 4545 Fragments arranged by size: 4545 1208 214 195 195 175 144 XmnI GAAnn'nnTTC Cuts at: 3835 4805 3835 Size: 970 5706 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2683 4484 276 Size: 53 83 186 936 1149 1801 2468 Fragments arranged by size: 2468 1801 1149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PsrI RsrII SapI SexAI SfiI SwaI