Restriction map for
pFN21AA0749
(Linear) (Six-base) MAPSORT of: KIAA0749.seq Check: 5454 from: 1 to: 1971
KIAA0749.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 464 1248 1971
Size: 464 784 723
Fragments arranged by size:
784 723 464
AatII G_ACGT'C
Cuts at: 0 680 1971
Size: 680 1291
AccI GT'mk_AC
Cuts at: 0 261 1971
Size: 261 1710
AflIII A'CryG_T
Cuts at: 0 46 1287 1971
Size: 46 1241 684
Fragments arranged by size:
1241 684 46
AhdI GACnn_n'nnGTC
Cuts at: 0 393 1971
Size: 393 1578
AlwNI CAG_nnn'CTG
Cuts at: 0 20 1049 1173 1708 1971
Size: 20 1029 124 535 263
Fragments arranged by size:
1029 535 263 124 20
ApaI G_GGCC'C
Cuts at: 0 140 243 413 437 1655 1668 1971
Size: 140 103 170 24 1218 13 303
Fragments arranged by size:
1218 303 170 140 103 24 13
AscI GG'CGCG_CC
Cuts at: 0 1620 1971
Size: 1620 351
AvaI C'yCGr_G
Cuts at: 0 60 158 255 290 430 574 682 948
Size: 60 98 97 35 140 144 108 266
Cuts at: 948 1782 1816 1971
Size: 834 34 155
Fragments arranged by size:
834 266 155 144 140 108 98 97
60 35 34
BamHI G'GATC_C
Cuts at: 0 64 657 724 1971
Size: 64 593 67 1247
Fragments arranged by size:
1247 593 67 64
BanI G'GyrC_C
Cuts at: 0 342 588 862 932 1063 1786 1971
Size: 342 246 274 70 131 723 185
Fragments arranged by size:
723 342 274 246 185 131 70
BanII G_rGCy'C
Cuts at: 0 27 140 159 243 253 278 314 413
Size: 27 113 19 84 10 25 36 99
Cuts at: 413 437 764 949 1056 1490 1578 1655 1668
Size: 24 327 185 107 434 88 77 13
Cuts at: 1668 1739 1971
Size: 71 232
Fragments arranged by size:
434 327 232 185 113 107 99 88
84 77 71 36 27 25 24 19
13 10
BbeI G_GCGC'C
Cuts at: 0 346 1790 1971
Size: 346 1444 181
Fragments arranged by size:
1444 346 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 1858 1971
Size: 1858 113
BbvCI CC'TCA_GC
Cuts at: 0 1430 1730 1971
Size: 1430 300 241
Fragments arranged by size:
1430 300 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 756 790 1971
Size: 756 34 1181
Fragments arranged by size:
1181 756 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1699 1971
Size: 1699 272
BglI GCCn_nnn'nGGC
Cuts at: 0 87 304 351 1421 1971
Size: 87 217 47 1070 550
Fragments arranged by size:
1070 550 217 87 47
BglII A'GATC_T
Cuts at: 0 974 1971
Size: 974 997
BlpI GC'TnA_GC
Cuts at: 0 1738 1971
Size: 1738 233
Bme1580I G_kGCm'C
Cuts at: 0 140 243 413 437 593 909 1146 1655
Size: 140 103 170 24 156 316 237 509
Cuts at: 1655 1668 1971
Size: 13 303
Fragments arranged by size:
509 316 303 237 170 156 140 103
24 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 1971
Size: 30 1941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1097 1971
Size: 1097 874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 602 1138 1480 1971
Size: 84 518 536 342 491
Fragments arranged by size:
536 518 491 342 84
Bpu10I CC'TnA_GC
Cuts at: 0 1430 1730 1971
Size: 1430 300 241
Fragments arranged by size:
1430 300 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1971
Size: 925 1046
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 700 718 1105 1971
Size: 202 498 18 387 866
Fragments arranged by size:
866 498 387 202 18
BsaAI yAC'GTr
Cuts at: 0 47 519 1211 1971
Size: 47 472 692 760
Fragments arranged by size:
760 692 472 47
BsaHI Gr'CG_yC
Cuts at: 0 184 343 492 677 1190 1205 1517 1787
Size: 184 159 149 185 513 15 312 270
Cuts at: 1787 1971
Size: 184
Fragments arranged by size:
513 312 270 185 184 184 159 149
15
BsaWI w'CCGG_w
Cuts at: 0 1066 1107 1971
Size: 1066 41 864
Fragments arranged by size:
1066 864 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 882 912 1971
Size: 882 30 1059
Fragments arranged by size:
1059 882 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1345 1477 1746 1747 1924 1971
Size: 1345 132 269 1 177 47
Fragments arranged by size:
1345 269 177 132 47 1
BseYI C'CCAG_C
Cuts at: 0 299 497 606 1253 1306 1560 1634 1971
Size: 299 198 109 647 53 254 74 337
Fragments arranged by size:
647 337 299 254 198 109 74 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1971
Size: 1282 689
BsiEI CG_ry'CG
Cuts at: 0 331 508 1629 1971
Size: 331 177 1121 342
Fragments arranged by size:
1121 342 331 177
BsiHKAI G_wGCw'C
Cuts at: 0 159 1547 1739 1971
Size: 159 1388 192 232
Fragments arranged by size:
1388 232 192 159
Bsp1286I G_dGCh'C
Cuts at: 0 27 140 159 243 253 278 314 413
Size: 27 113 19 84 10 25 36 99
Cuts at: 413 437 593 764 909 949 1056 1146 1490
Size: 24 156 171 145 40 107 90 344
Cuts at: 1490 1547 1578 1655 1668 1739 1971
Size: 57 31 77 13 71 232
Fragments arranged by size:
344 232 171 156 145 113 107 99
90 84 77 71 57 40 36 31
27 25 24 19 13 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 464 1248 1517 1971
Size: 464 784 269 454
Fragments arranged by size:
784 464 454 269
BsrBI CCG'CTC
Cuts at: 0 195 742 919 1971
Size: 195 547 177 1052
Fragments arranged by size:
1052 547 195 177
BsrFI r'CCGG_y
Cuts at: 0 86 503 1624 1971
Size: 86 417 1121 347
Fragments arranged by size:
1121 417 347 86
BssHII G'CGCG_C
Cuts at: 0 1620 1971
Size: 1620 351
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1269 1971
Size: 1269 702
BstYI r'GATC_y
Cuts at: 0 64 657 665 724 925 974 1883 1971
Size: 64 593 8 59 201 49 909 88
Fragments arranged by size:
909 593 201 88 64 59 49 8
Bsu36I CC'TnA_GG
Cuts at: 0 1554 1971
Size: 1554 417
BtgI C'CryG_G
Cuts at: 0 1179 1346 1416 1971
Size: 1179 167 70 555
Fragments arranged by size:
1179 555 167 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 216 251 1971
Size: 216 35 1720
Fragments arranged by size:
1720 216 35
EaeI y'GGCC_r
Cuts at: 0 143 328 505 743 1626 1971
Size: 143 185 177 238 883 345
Fragments arranged by size:
883 345 238 185 177 143
EagI C'GGCC_G
Cuts at: 0 328 505 1626 1971
Size: 328 177 1121 345
Fragments arranged by size:
1121 345 328 177
EarI CTCTTCn'nnn_
Cuts at: 0 643 1924 1957 1971
Size: 643 1281 33 14
Fragments arranged by size:
1281 643 33 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1681 1812 1971
Size: 1681 131 159
Fragments arranged by size:
1681 159 131
EcoICRI GAG'CTC
Cuts at: 0 157 1737 1971
Size: 157 1580 234
Fragments arranged by size:
1580 234 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 602 1138 1480 1971
Size: 84 518 536 342 491
Fragments arranged by size:
536 518 491 342 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 1816 1971
Size: 145 1671 155
Fragments arranged by size:
1671 155 145
EcoO109I rG'GnC_Cy
Cuts at: 0 136 137 239 409 410 434 541 551
Size: 136 1 102 170 1 24 107 10
Cuts at: 551 571 1170 1651 1652 1664 1665 1808 1914
Size: 20 599 481 1 12 1 143 106
Cuts at: 1914 1943 1971
Size: 29 28
Fragments arranged by size:
599 481 170 143 136 107 106 102
29 28 24 20 12 10 1 1
1 1
HaeII r_GCGC'y
Cuts at: 0 339 346 361 1790 1971
Size: 339 7 15 1429 181
Fragments arranged by size:
1429 339 181 15 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 642 674 959 991 1971
Size: 379 32 231 32 285 32 980
Fragments arranged by size:
980 379 285 231 32 32 32
HincII GTy'rAC
Cuts at: 0 262 1971
Size: 262 1709
KasI G'GCGC_C
Cuts at: 0 342 1786 1971
Size: 342 1444 185
Fragments arranged by size:
1444 342 185
MluI A'CGCG_T
Cuts at: 0 1287 1971
Size: 1287 684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 1105 1264 1577 1803 1971
Size: 723 382 159 313 226 168
Fragments arranged by size:
723 382 313 226 168 159
MspA1I CmG'CkG
Cuts at: 0 303 497 610 1418 1617 1858 1971
Size: 303 194 113 808 199 241 113
Fragments arranged by size:
808 303 241 199 194 113 113
NaeI GCC'GGC
Cuts at: 0 88 1626 1971
Size: 88 1538 345
Fragments arranged by size:
1538 345 88
NarI GG'CG_CC
Cuts at: 0 343 1787 1971
Size: 343 1444 184
Fragments arranged by size:
1444 343 184
NgoMIV G'CCGG_C
Cuts at: 0 86 1624 1971
Size: 86 1538 347
Fragments arranged by size:
1538 347 86
PasI CC'CwG_GG
Cuts at: 0 92 391 413 1049 1167 1578 1648 1971
Size: 92 299 22 636 118 411 70 323
Fragments arranged by size:
636 411 323 299 118 92 70 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 20 736 992 1049 1971
Size: 20 716 256 57 922
Fragments arranged by size:
922 716 256 57 20
PfoI T'CCnGG_A
Cuts at: 0 1056 1971
Size: 1056 915
PmlI CAC'GTG
Cuts at: 0 47 1211 1971
Size: 47 1164 760
Fragments arranged by size:
1164 760 47
PpuMI rG'GwC_Cy
Cuts at: 0 551 1808 1914 1943 1971
Size: 551 1257 106 29 28
Fragments arranged by size:
1257 551 106 29 28
PspOMI G'GGCC_C
Cuts at: 0 136 239 409 433 1651 1664 1971
Size: 136 103 170 24 1218 13 307
Fragments arranged by size:
1218 307 170 136 103 24 13
PspXI vC'TCGA_Gb
Cuts at: 0 948 1971
Size: 948 1023
PstI C_TGCA'G
Cuts at: 0 113 1971
Size: 113 1858
PvuII CAG'CTG
Cuts at: 0 303 610 1971
Size: 303 307 1361
Fragments arranged by size:
1361 307 303
SacI G_AGCT'C
Cuts at: 0 159 1739 1971
Size: 159 1580 232
Fragments arranged by size:
1580 232 159
SacII CC_GC'GG
Cuts at: 0 1419 1971
Size: 1419 552
SalI G'TCGA_C
Cuts at: 0 260 1971
Size: 260 1711
SanDI GG'GwC_CC
Cuts at: 0 1943 1971
Size: 1943 28
SfcI C'TryA_G
Cuts at: 0 109 848 1971
Size: 109 739 1123
Fragments arranged by size:
1123 739 109
SfoI GGC'GCC
Cuts at: 0 344 1788 1971
Size: 344 1444 183
Fragments arranged by size:
1444 344 183
SmaI CCC'GGG
Cuts at: 0 62 257 432 576 1784 1971
Size: 62 195 175 144 1208 187
Fragments arranged by size:
1208 195 187 175 144 62
SmlI C'TyrA_G
Cuts at: 0 940 948 1971
Size: 940 8 1023
Fragments arranged by size:
1023 940 8
SrfI GCCC'GGGC
Cuts at: 0 432 1971
Size: 432 1539
StyI C'CwwG_G
Cuts at: 0 203 554 1971
Size: 203 351 1417
Fragments arranged by size:
1417 351 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 1971
Size: 226 1745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 1971
Size: 1338 32 601
Fragments arranged by size:
1338 601 32
Tth111I GACn'n_nGTC
Cuts at: 0 1873 1971
Size: 1873 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 398 494 1971
Size: 398 96 1477
Fragments arranged by size:
1477 398 96
XhoI C'TCGA_G
Cuts at: 0 948 1971
Size: 948 1023
XmaI C'CCGG_G
Cuts at: 0 60 255 430 574 1782 1971
Size: 60 195 175 144 1208 189
Fragments arranged by size:
1208 195 189 175 144 60
XmnI GAAnn'nnTTC
Cuts at: 0 1830 1971
Size: 1830 141
ZraI GAC'GTC
Cuts at: 0 678 1971
Size: 678 1293
Enzymes that do cut:
AarI AatII AccI AflIII AhdI AlwNI ApaI AscI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstYI Bsu36I BtgI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MluI MmeI MspA1I NaeI NarI NgoMIV
PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SalI SanDI SfcI SfoI SmaI
SmlI SrfI StyI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AleI AlfI
AloI ApaLI ApoI AseI AsiSI AvrII BaeI BclI
BfrBI BmgBI BmtI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TatI
XbaI