(Circular) (Six-base) MAPSORT of: pFN21AB8579.seq Check: 8845 from: 1 to: 5401 pFN21AB8579 5401 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2451 3211 278 Size: 53 83 186 936 915 760 2468 Fragments arranged by size: 2468 936 915 760 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2599 3464 4848 5116 1632 Size: 967 865 1384 268 1917 Fragments arranged by size: 1917 1384 967 865 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5204 AflIII A'CryG_T Cuts at: 1203 4300 5277 1203 Size: 3097 977 1327 Fragments arranged by size: 3097 1327 977 AgeI A'CCGG_T Cuts at: 4232 4232 Size: 5401 AhdI GACnn_n'nnGTC Cuts at: 2167 4130 2167 Size: 1963 3438 AleI CACnn'nnGTG Cuts at: 1166 2280 1166 Size: 1114 4287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5401 AlwNI CAG_nnn'CTG Cuts at: 2302 2578 2626 3144 4716 2302 Size: 276 48 518 1572 2987 Fragments arranged by size: 2987 1572 518 276 48 ApaLI G'TGCA_C Cuts at: 3458 4614 3458 Size: 1156 4245 ApoI r'AATT_y Cuts at: 1493 2710 2936 3088 5197 1493 Size: 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2726 1870 Size: 73 175 608 4545 Fragments arranged by size: 4545 608 175 73 BamHI G'GATC_C Cuts at: 2731 3098 2731 Size: 367 5034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2512 2722 4177 618 Size: 325 217 627 725 210 1455 1842 Fragments arranged by size: 1842 1455 725 627 325 217 210 BanII G_rGCy'C Cuts at: 1391 2159 2336 2720 1391 Size: 768 177 384 4072 Fragments arranged by size: 4072 768 384 177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 928 Size: 971 315 4115 Fragments arranged by size: 4115 971 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3592 3626 1903 Size: 34 50 34 1571 34 3678 Fragments arranged by size: 3678 1571 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2385 4012 136 Size: 107 122 71 631 605 713 1627 1525 Fragments arranged by size: 1627 1525 713 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2098 2633 2818 2098 Size: 535 185 4681 Fragments arranged by size: 4681 535 185 Bme1580I G_kGCm'C Cuts at: 1790 2517 3462 4618 1790 Size: 727 945 1156 2573 Fragments arranged by size: 2573 1156 945 727 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5401 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 4090 442 Size: 416 53 83 399 24 1715 958 1753 Fragments arranged by size: 1753 1715 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 4061 1239 Size: 932 1890 2579 Fragments arranged by size: 2579 1890 932 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3526 4391 4689 3526 Size: 865 298 4238 Fragments arranged by size: 4238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4064 882 Size: 617 2565 2219 Fragments arranged by size: 2565 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3192 493 Size: 711 1988 2702 Fragments arranged by size: 2702 1988 711 BsaBI GATnn'nnATC Cuts at: 5395 5395 Size: 5401 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2341 2448 3208 3590 Size: 53 83 186 936 808 107 760 382 Cuts at: 3590 4225 275 Size: 635 1451 Fragments arranged by size: 1451 936 808 760 635 382 186 107 83 53 BsaWI w'CCGG_w Cuts at: 3834 4232 4506 4653 5064 3834 Size: 398 274 147 411 4171 Fragments arranged by size: 4171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 4435 Fragments arranged by size: 4435 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2138 2708 1186 Size: 849 103 570 3879 Fragments arranged by size: 3879 849 570 103 BseYI C'CCAG_C Cuts at: 2329 2504 4604 5100 2329 Size: 175 2100 496 2630 Fragments arranged by size: 2630 2100 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4828 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2090 2851 3612 4640 1540 Size: 275 186 89 761 761 1028 2301 Fragments arranged by size: 2301 1028 761 761 275 186 89 BsiHKAI G_wGCw'C Cuts at: 2159 2639 3462 3547 4618 2159 Size: 480 823 85 1071 2942 Fragments arranged by size: 2942 1071 823 480 85 BsmI GAATG_Cn' Cuts at: 2912 3005 2912 Size: 93 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2336 2517 2639 2720 3462 3547 Size: 399 369 177 181 122 81 742 85 Cuts at: 3547 4618 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 181 177 122 85 81 BspEI T'CCGG_A Cuts at: 5064 5064 Size: 5401 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 844 Size: 1912 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2139 3288 1394 Size: 429 316 1149 3507 Fragments arranged by size: 3507 1149 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4045 4232 1700 Size: 110 2235 187 2869 Fragments arranged by size: 2869 2235 187 110 BsrGI T'GTAC_A Cuts at: 96 2667 96 Size: 2571 2830 BssHII G'CGCG_C Cuts at: 1925 2348 1925 Size: 423 4978 BssSI C'ACGA_G Cuts at: 2143 2534 3461 4473 2143 Size: 391 927 1012 3071 Fragments arranged by size: 3071 1012 927 391 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2472 794 Size: 1508 170 3723 Fragments arranged by size: 3723 1508 170 BstBI TT'CG_AA Cuts at: 4205 4205 Size: 5401 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5401 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2149 Size: 5401 BstYI r'GATC_y Cuts at: 1972 2568 2731 3098 3483 3500 4952 5038 5050 Size: 596 163 367 385 17 1452 86 12 Cuts at: 5050 5396 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 596 385 367 346 163 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2265 2265 Size: 5401 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4849 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5366 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DraIII CAC_nnn'GTG Cuts at: 2482 2482 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4408 809 Size: 507 3092 1802 Fragments arranged by size: 3092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2087 2848 3737 Size: 54 1425 50 275 18 257 761 889 Cuts at: 3737 5366 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 761 275 257 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2087 2848 1537 Size: 275 275 761 4090 Fragments arranged by size: 4090 761 275 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 3331 1353 Size: 700 1278 3423 Fragments arranged by size: 3423 1278 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2578 3988 4362 4508 387 Size: 1064 138 989 1410 374 146 1280 Fragments arranged by size: 1410 1280 1064 989 374 146 138 EcoICRI GAG'CTC Cuts at: 2157 2157 Size: 5401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2599 3464 4061 4848 5116 1239 Size: 393 539 428 865 597 787 268 1524 Fragments arranged by size: 1524 865 787 597 539 428 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2318 2318 Size: 5401 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 FspI TGC'GCA Cuts at: 3907 3907 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 2441 4548 1678 Size: 763 2107 2531 Fragments arranged by size: 2531 2107 763 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2153 2185 2216 Size: 32 135 32 440 32 109 32 31 Cuts at: 2216 2248 4042 4074 4116 4148 1373 Size: 32 1794 32 42 32 2626 Fragments arranged by size: 2626 1794 440 135 109 42 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5401 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 3000 3000 Size: 5401 MluI A'CGCG_T Cuts at: 5277 5277 Size: 5401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4515 4699 1305 Size: 3210 184 2007 Fragments arranged by size: 3210 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3579 Fragments arranged by size: 3579 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 3359 3718 3877 518 Size: 648 1114 1079 359 159 2042 Fragments arranged by size: 2042 1114 1079 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2491 2527 2683 2817 3494 4642 4887 Size: 693 192 36 156 134 677 1148 245 Cuts at: 4887 1606 Size: 2120 Fragments arranged by size: 2120 1148 693 677 245 192 156 134 36 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4849 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2759 4304 1132 Size: 97 1530 1545 2229 Fragments arranged by size: 2229 1545 1530 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5401 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 1108 Size: 1374 4027 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PstI C_TGCA'G Cuts at: 830 2307 2753 830 Size: 1477 446 3478 Fragments arranged by size: 3478 1477 446 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 PvuII CAG'CTG Cuts at: 2299 2491 2527 2683 2299 Size: 192 36 156 5017 Fragments arranged by size: 5017 192 156 36 SacI G_AGCT'C Cuts at: 2159 2159 Size: 5401 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2303 2410 2749 3884 4565 4756 826 Size: 219 1258 107 339 1135 681 191 1471 Fragments arranged by size: 1471 1258 1135 681 339 219 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2120 2728 1872 Size: 248 608 4545 Fragments arranged by size: 4545 608 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 3505 4406 4668 820 Size: 197 926 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3192 493 Size: 2699 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2759 1132 Size: 1627 3774 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5401 StyI C'CwwG_G Cuts at: 513 1065 2840 513 Size: 552 1775 3074 Fragments arranged by size: 3074 1775 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3428 3445 3598 3783 4228 325 Size: 1255 1848 17 153 185 445 1498 Fragments arranged by size: 1848 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 3647 5359 Size: 274 80 33 51 1443 690 980 1712 Cuts at: 5359 96 Size: 138 Fragments arranged by size: 1712 1443 980 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5361 5393 5361 Size: 32 5369 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5401 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5401 XmaI C'CCGG_G Cuts at: 1870 2118 2726 1870 Size: 248 608 4545 Fragments arranged by size: 4545 608 248 XmnI GAAnn'nnTTC Cuts at: 3530 3530 Size: 5401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2449 3209 276 Size: 53 83 186 936 915 760 2468 Fragments arranged by size: 2468 936 915 760 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI