(Circular) (Six-base) MAPSORT of: pFN21AB6822.seq Check: 8945 from: 1 to: 5263 pFN21AB6822 5263 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2116 2302 2116 Size: 186 5077 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3073 278 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 1937 Size: 669 4594 Acc65I G'GTAC_C Cuts at: 2584 2584 Size: 5263 AclI AA'CG_TT Cuts at: 1563 3390 3763 1563 Size: 1827 373 3063 Fragments arranged by size: 3063 1827 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3326 4710 4978 1632 Size: 1694 1384 268 1917 Fragments arranged by size: 1917 1694 1384 268 AfeI AGC'GCT Cuts at: 2060 2387 2060 Size: 327 4936 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5066 AflIII A'CryG_T Cuts at: 1203 4162 5139 1203 Size: 2959 977 1327 Fragments arranged by size: 2959 1327 977 AgeI A'CCGG_T Cuts at: 4094 4094 Size: 5263 AhdI GACnn_n'nnGTC Cuts at: 3992 3992 Size: 5263 AleI CACnn'nnGTG Cuts at: 1166 2543 1166 Size: 1377 3886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 1184 Size: 1150 4113 AlwNI CAG_nnn'CTG Cuts at: 3006 4578 3006 Size: 1572 3691 ApaLI G'TGCA_C Cuts at: 3320 4476 3320 Size: 1156 4107 ApoI r'AATT_y Cuts at: 1493 2572 2798 2950 5059 1493 Size: 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 3817 160 Size: 872 2785 1606 Fragments arranged by size: 2785 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2588 1870 Size: 73 97 548 4545 Fragments arranged by size: 4545 548 97 73 BamHI G'GATC_C Cuts at: 2209 2593 2960 2209 Size: 384 367 4512 Fragments arranged by size: 4512 384 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2584 4039 618 Size: 325 217 627 539 258 1455 1842 Fragments arranged by size: 1842 1455 627 539 325 258 217 BanII G_rGCy'C Cuts at: 1391 2582 1391 Size: 1191 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2213 2247 3454 3488 1903 Size: 34 276 34 1207 34 3678 Fragments arranged by size: 3678 1207 276 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3155 4365 3155 Size: 1210 4053 BclI T'GATC_A Cuts at: 1548 2196 1548 Size: 648 4615 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3874 136 Size: 107 122 71 631 605 2202 1525 Fragments arranged by size: 2202 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2191 5258 2191 Size: 3067 2196 BlpI GC'TnA_GC Cuts at: 2680 2680 Size: 5263 Bme1580I G_kGCm'C Cuts at: 1790 2331 3324 4480 1790 Size: 541 993 1156 2573 Fragments arranged by size: 2573 1156 993 541 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2994 3952 442 Size: 416 53 83 399 24 1577 958 1753 Fragments arranged by size: 1753 1577 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5263 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2327 2477 3923 1239 Size: 836 252 150 1446 2579 Fragments arranged by size: 2579 1446 836 252 150 Bpu10I CC'TnA_GC Cuts at: 2364 2364 Size: 5263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3388 4253 4551 3388 Size: 865 298 4100 Fragments arranged by size: 4100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3926 882 Size: 617 2427 2219 Fragments arranged by size: 2427 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3054 493 Size: 711 1850 2702 Fragments arranged by size: 2702 1850 711 BsaBI GATnn'nnATC Cuts at: 5257 5257 Size: 5263 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3070 3452 4087 275 Size: 53 83 186 936 1537 382 635 1451 Fragments arranged by size: 1537 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3696 4094 4368 4515 4926 3696 Size: 398 274 147 411 4033 Fragments arranged by size: 4033 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5038 Fragments arranged by size: 5038 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2453 2573 1186 Size: 1102 165 120 3876 Fragments arranged by size: 3876 1102 165 120 BseYI C'CCAG_C Cuts at: 2061 4466 4962 2061 Size: 2405 496 2362 Fragments arranged by size: 2405 2362 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2522 1163 Size: 573 408 378 3904 Fragments arranged by size: 3904 573 408 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2227 2713 3474 4502 1540 Size: 275 186 226 486 761 1028 2301 Fragments arranged by size: 2301 1028 761 486 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2347 3324 3409 4480 2347 Size: 977 85 1071 3130 Fragments arranged by size: 3130 1071 977 85 BsmI GAATG_Cn' Cuts at: 2323 2774 2867 2323 Size: 451 93 4719 Fragments arranged by size: 4719 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2347 2582 3324 3409 4480 1391 Size: 399 541 16 235 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 235 85 16 BspEI T'CCGG_A Cuts at: 4926 4926 Size: 5263 BspHI T'CATG_A Cuts at: 3152 4882 3152 Size: 1730 3533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 2302 2618 844 Size: 1272 186 316 3489 Fragments arranged by size: 3489 1272 316 186 BsrBI CCG'CTC Cuts at: 1394 1823 3150 1394 Size: 429 1327 3507 Fragments arranged by size: 3507 1327 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 3758 3932 65 Size: 1183 981 1529 174 1396 Fragments arranged by size: 1529 1396 1183 981 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3907 4094 1700 Size: 110 2097 187 2869 Fragments arranged by size: 2869 2097 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5263 BssHII G'CGCG_C Cuts at: 1925 2084 1925 Size: 159 5104 BssSI C'ACGA_G Cuts at: 3323 4335 3323 Size: 1012 4251 BstAPI GCAn_nnn'nTGC Cuts at: 794 2500 794 Size: 1706 3557 BstBI TT'CG_AA Cuts at: 4067 4067 Size: 5263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5263 BstYI r'GATC_y Cuts at: 1972 2191 2209 2397 2593 2960 3345 3362 4814 Size: 219 18 188 196 367 385 17 1452 Cuts at: 4814 4900 4912 5258 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 219 196 188 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3772 Fragments arranged by size: 3772 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4524 Fragments arranged by size: 4524 683 56 BtsI GCAGTG_nn' Cuts at: 2510 2779 3573 3593 2510 Size: 269 794 20 4180 Fragments arranged by size: 4180 794 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5228 DraI TTT'AAA Cuts at: 2567 2567 Size: 5263 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2114 4270 809 Size: 507 798 2156 1802 Fragments arranged by size: 2156 1802 798 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2710 3599 5228 Size: 54 1425 50 275 18 880 889 1629 Cuts at: 5228 8 Size: 43 Fragments arranged by size: 1629 1425 889 880 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2710 1537 Size: 275 898 4090 Fragments arranged by size: 4090 898 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 3193 1353 Size: 690 1150 3423 Fragments arranged by size: 3423 1150 690 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3850 4224 4370 387 Size: 1064 138 2261 374 146 1280 Fragments arranged by size: 2261 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2327 2477 3326 3923 4710 4978 Size: 393 443 252 150 849 597 787 268 Cuts at: 4978 1239 Size: 1524 Fragments arranged by size: 1524 849 787 597 443 393 268 252 150 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2270 2291 2309 2117 Size: 153 21 18 5071 Fragments arranged by size: 5071 153 21 18 EcoRI G'AATT_C Cuts at: 2572 2950 2572 Size: 378 4885 FspI TGC'GCA Cuts at: 3769 3769 Size: 5263 HaeII r_GCGC'y Cuts at: 1678 2062 2389 4410 1678 Size: 384 327 2021 2531 Fragments arranged by size: 2531 2021 384 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2176 2208 3904 3936 3978 Size: 32 135 32 604 32 1696 32 42 Cuts at: 3978 4010 1373 Size: 32 2626 Fragments arranged by size: 2626 1696 604 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2607 2853 669 Size: 988 281 669 246 3079 Fragments arranged by size: 3079 988 669 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5263 HpaI GTT'AAC Cuts at: 2853 2853 Size: 5263 KpnI G_GTAC'C Cuts at: 2588 2588 Size: 5263 MfeI C'AATT_G Cuts at: 2862 2862 Size: 5263 MluI A'CGCG_T Cuts at: 5139 5139 Size: 5263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4377 4561 1305 Size: 3072 184 2007 Fragments arranged by size: 3072 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3441 Fragments arranged by size: 3441 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3221 3580 3739 518 Size: 648 1377 678 359 159 2042 Fragments arranged by size: 2042 1377 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2679 3356 4504 4749 1606 Size: 423 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 650 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3772 Fragments arranged by size: 3772 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5263 NotI GC'GGCC_GC Cuts at: 2710 2710 Size: 5263 NspI r_CATG'y Cuts at: 1132 1229 2372 2621 4166 1132 Size: 97 1143 249 1545 2229 Fragments arranged by size: 2229 1545 1143 249 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5263 PciI A'CATG_T Cuts at: 4162 4162 Size: 5263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 1108 Size: 1317 3946 PfoI T'CCnGG_A Cuts at: 2304 2454 2304 Size: 150 5113 PmeI GTTT'AAAC Cuts at: 2567 2567 Size: 5263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3332 3364 4845 4877 3332 Size: 32 1481 32 3718 Fragments arranged by size: 3718 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5263 PsiI TTA'TAA Cuts at: 2833 2833 Size: 5263 PstI C_TGCA'G Cuts at: 830 2385 2615 830 Size: 1555 230 3478 Fragments arranged by size: 3478 1555 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5263 SalI G'TCGA_C Cuts at: 1936 2605 1936 Size: 669 4594 SapI GCTCTTCn'nnn_ Cuts at: 2043 2043 Size: 5263 SbfI CC_TGCA'GG Cuts at: 2615 2615 Size: 5263 ScaI AGT'ACT Cuts at: 3511 5223 3511 Size: 1712 3551 SfcI C'TryA_G Cuts at: 826 1045 2033 2381 2611 3746 4427 4618 826 Size: 219 988 348 230 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 681 348 230 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5263 SmaI CCC'GGG Cuts at: 1872 2590 1872 Size: 718 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3367 4268 4530 820 Size: 197 926 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3054 493 Size: 2561 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5263 SphI G_CATG'C Cuts at: 1132 2372 2621 1132 Size: 1240 249 3774 Fragments arranged by size: 3774 1240 249 SspI AAT'ATT Cuts at: 5 52 3187 5 Size: 47 3135 2081 Fragments arranged by size: 3135 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5263 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2445 2702 513 Size: 552 939 318 123 257 3074 Fragments arranged by size: 3074 939 552 318 257 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2053 3290 3307 3460 3645 4090 325 Size: 1255 473 1237 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1237 473 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 3509 5221 Size: 274 80 33 51 1443 240 1292 1712 Cuts at: 5221 96 Size: 138 Fragments arranged by size: 1712 1443 1292 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5223 5255 5223 Size: 32 5231 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5263 XbaI T'CTAG_A Cuts at: 2599 2599 Size: 5263 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2452 2452 Size: 5263 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5263 XmaI C'CCGG_G Cuts at: 1870 2588 1870 Size: 718 4545 XmnI GAAnn'nnTTC Cuts at: 3392 3392 Size: 5263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3071 276 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI