(Circular) (Six-base) MAPSORT of: pFN21AB6509.seq Check: 9173 from: 1 to: 5782 pFN21AB6509 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2895 3045 3125 1937 Size: 958 150 80 4594 Fragments arranged by size: 4594 958 150 80 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 2444 2447 2582 3019 3845 5229 5497 Size: 704 108 3 135 437 826 1384 268 Cuts at: 5497 1632 Size: 1917 Fragments arranged by size: 1917 1384 826 704 437 268 135 108 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2495 1166 Size: 1329 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2467 3525 5097 2467 Size: 1058 1572 3152 Fragments arranged by size: 3152 1572 1058 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 AvrII C'CTAG_G Cuts at: 2152 2152 Size: 5782 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2838 2871 2838 Size: 33 5749 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 3103 4558 618 Size: 325 217 627 762 554 1455 1842 Fragments arranged by size: 1842 1455 762 627 554 325 217 BanII G_rGCy'C Cuts at: 1391 2148 2570 3101 1391 Size: 757 422 531 4072 Fragments arranged by size: 4072 757 531 422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2651 928 Size: 971 752 4059 Fragments arranged by size: 4059 971 752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4393 136 Size: 107 122 71 631 605 337 2384 1525 Fragments arranged by size: 2384 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2168 2476 2552 3843 4999 1790 Size: 378 308 76 1291 1156 2573 Fragments arranged by size: 2573 1291 1156 378 308 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2153 2555 3022 Size: 416 53 83 399 24 736 402 467 Cuts at: 3022 3513 4471 442 Size: 491 958 1753 Fragments arranged by size: 1753 958 736 491 467 416 402 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2724 1056 Size: 1668 4114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2391 4442 1239 Size: 1152 2051 2579 Fragments arranged by size: 2579 2051 1152 Bpu10I CC'TnA_GC Cuts at: 2569 2569 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2637 3907 4772 5070 2637 Size: 1270 865 298 3349 Fragments arranged by size: 3349 1270 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3049 3573 493 Size: 711 1845 524 2702 Fragments arranged by size: 2702 1845 711 524 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5782 BseYI C'CCAG_C Cuts at: 4985 5481 4985 Size: 496 5286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2570 3843 3928 4999 2570 Size: 1273 85 1071 3353 Fragments arranged by size: 3353 1273 1071 85 BsmI GAATG_Cn' Cuts at: 2646 3293 3386 2646 Size: 647 93 5042 Fragments arranged by size: 5042 647 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2168 2476 2552 2570 3101 3843 Size: 399 358 20 308 76 18 531 742 Cuts at: 3843 3928 4999 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 358 308 85 76 20 18 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 3137 844 Size: 1430 863 3489 Fragments arranged by size: 3489 1430 863 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2622 4277 4451 65 Size: 1183 1374 1655 174 1396 Fragments arranged by size: 1655 1396 1374 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2236 3112 3479 3864 3881 5333 5419 5431 Size: 264 876 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 876 385 367 346 264 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2799 3229 4118 Size: 54 1425 50 275 18 969 430 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 969 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2463 2558 3712 1353 Size: 1110 95 1154 3423 Fragments arranged by size: 3423 1154 1110 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2568 2568 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2391 2444 2447 2582 3019 3845 Size: 393 704 55 53 3 135 437 826 Cuts at: 3845 4442 5229 5497 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 826 787 704 597 437 393 268 135 55 53 3 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2809 4423 4455 4497 Size: 32 135 32 1205 32 1614 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1614 1205 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 2785 3126 3372 669 Size: 988 281 406 441 341 246 3079 Fragments arranged by size: 3079 988 441 406 341 281 246 HindIII A'AGCT_T Cuts at: 748 2843 748 Size: 2095 3687 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2905 4896 5080 1305 Size: 1071 529 1991 184 2007 Fragments arranged by size: 2007 1991 1071 529 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 10 Size: 54 1425 343 969 2991 Fragments arranged by size: 2991 1425 969 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 2495 2688 3740 4099 4258 518 Size: 648 1315 14 193 1052 359 159 2042 Fragments arranged by size: 2042 1315 1052 648 359 193 159 14 MspA1I CmG'CkG Cuts at: 1606 2175 2425 2560 3198 3875 5023 5268 1606 Size: 569 250 135 638 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 638 569 250 245 135 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 2720 1052 Size: 1668 4114 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PfoI T'CCnGG_A Cuts at: 2368 2368 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 3134 830 Size: 2304 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2425 2425 Size: 5782 SacI G_AGCT'C Cuts at: 2570 2570 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2558 2558 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 3011 4030 5742 3011 Size: 1019 1712 3051 Fragments arranged by size: 3051 1712 1019 SexAI A'CCwGG_T Cuts at: 2857 2857 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 2919 3130 4265 4946 5137 826 Size: 219 1874 211 1135 681 191 1471 Fragments arranged by size: 1874 1471 1135 681 219 211 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2652 3886 4787 5049 820 Size: 197 926 709 1234 901 262 1553 Fragments arranged by size: 1553 1234 926 901 709 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2152 3221 513 Size: 552 1087 1069 3074 Fragments arranged by size: 3074 1087 1069 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3809 3826 3979 4164 4609 325 Size: 1255 2229 17 153 185 445 1498 Fragments arranged by size: 2229 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 3009 4028 Size: 274 80 33 51 1443 903 129 1019 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 903 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2632 2664 5742 5774 2632 Size: 32 3078 32 2640 Fragments arranged by size: 3078 2640 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI