(Circular) (Six-base) MAPSORT of: pFN21AE0508.seq Check: 1059 from: 1 to: 5209 pFN21AE0508 5209 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3019 278 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2552 1937 Size: 615 4594 Acc65I G'GTAC_C Cuts at: 2530 2530 Size: 5209 AclI AA'CG_TT Cuts at: 1563 3336 3709 1563 Size: 1773 373 3063 Fragments arranged by size: 3063 1773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2320 2424 3272 4656 4924 1632 Size: 688 104 848 1384 268 1917 Fragments arranged by size: 1917 1384 848 688 268 104 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5012 AflIII A'CryG_T Cuts at: 1203 2040 4108 5085 1203 Size: 837 2068 977 1327 Fragments arranged by size: 2068 1327 977 837 AgeI A'CCGG_T Cuts at: 4040 4040 Size: 5209 AhdI GACnn_n'nnGTC Cuts at: 3938 3938 Size: 5209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5209 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 2273 Size: 32 5177 AlwNI CAG_nnn'CTG Cuts at: 2213 2359 2952 4524 2213 Size: 146 593 1572 2898 Fragments arranged by size: 2898 1572 593 146 ApaLI G'TGCA_C Cuts at: 2132 3266 4422 2132 Size: 1134 1156 2919 Fragments arranged by size: 2919 1156 1134 ApoI r'AATT_y Cuts at: 1493 2518 2744 2896 5005 1493 Size: 1025 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 226 152 AseI AT'TA_AT Cuts at: 160 1032 3763 160 Size: 872 2731 1606 Fragments arranged by size: 2731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 AvaI C'yCGr_G Cuts at: 1870 1943 2182 2534 1870 Size: 73 239 352 4545 Fragments arranged by size: 4545 352 239 73 BamHI G'GATC_C Cuts at: 2539 2906 2539 Size: 367 4842 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2530 3985 618 Size: 325 217 627 743 1455 1842 Fragments arranged by size: 1842 1455 743 627 325 217 BanII G_rGCy'C Cuts at: 1391 2483 2528 1391 Size: 1092 45 4072 Fragments arranged by size: 4072 1092 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3400 3434 1903 Size: 34 1463 34 3678 Fragments arranged by size: 3678 1463 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4311 3101 Size: 1210 3999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3820 136 Size: 107 122 71 631 605 2148 1525 Fragments arranged by size: 2148 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5204 5204 Size: 5209 BlpI GC'TnA_GC Cuts at: 2075 2626 2075 Size: 551 4658 Bme1580I G_kGCm'C Cuts at: 1790 2136 3270 4426 1790 Size: 346 1134 1156 2573 Fragments arranged by size: 2573 1156 1134 346 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5209 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 2940 3898 Size: 416 53 83 399 24 956 567 958 Cuts at: 3898 442 Size: 1753 Fragments arranged by size: 1753 958 956 567 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5209 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 5209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2190 2279 2454 3869 1239 Size: 916 35 89 175 1415 2579 Fragments arranged by size: 2579 1415 916 175 89 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3334 4199 4497 3334 Size: 865 298 4046 Fragments arranged by size: 4046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3872 882 Size: 617 2373 2219 Fragments arranged by size: 2373 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 3000 493 Size: 711 837 959 2702 Fragments arranged by size: 2702 959 837 711 BsaBI GATnn'nnATC Cuts at: 5203 5203 Size: 5209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3016 3398 4033 275 Size: 53 83 186 936 1483 382 635 1451 Fragments arranged by size: 1483 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2026 3642 4040 4314 4461 4872 2026 Size: 1616 398 274 147 411 2363 Fragments arranged by size: 2363 1616 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2273 2285 2303 1178 Size: 30 165 30 852 18 12 18 4084 Fragments arranged by size: 4084 852 165 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 1186 Size: 1074 4135 BseYI C'CCAG_C Cuts at: 2089 4412 4908 2089 Size: 2323 496 2390 Fragments arranged by size: 2390 2323 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4636 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3420 4448 1540 Size: 275 186 658 761 1028 2301 Fragments arranged by size: 2301 1028 761 658 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2483 3270 3355 4426 2136 Size: 347 787 85 1071 2919 Fragments arranged by size: 2919 1071 787 347 85 BsmI GAATG_Cn' Cuts at: 2720 2813 2720 Size: 93 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2483 2528 3270 3355 4426 1391 Size: 399 346 347 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 347 346 85 45 BspEI T'CCGG_A Cuts at: 4872 4872 Size: 5209 BspHI T'CATG_A Cuts at: 3098 4828 3098 Size: 1730 3479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 2564 844 Size: 1559 161 3489 Fragments arranged by size: 3489 1559 161 BsrBI CCG'CTC Cuts at: 1394 1823 3096 1394 Size: 429 1273 3507 Fragments arranged by size: 3507 1273 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2192 3704 3878 65 Size: 1183 944 1512 174 1396 Fragments arranged by size: 1512 1396 1183 944 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3853 4040 1700 Size: 110 2043 187 2869 Fragments arranged by size: 2869 2043 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5209 BssSI C'ACGA_G Cuts at: 3269 4281 3269 Size: 1012 4197 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5209 BstBI TT'CG_AA Cuts at: 4013 4013 Size: 5209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5209 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2089 2062 Size: 27 5182 BstYI r'GATC_y Cuts at: 1972 2460 2539 2906 3291 3308 4760 4846 4858 Size: 488 79 367 385 17 1452 86 12 Cuts at: 4858 5204 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 488 385 367 346 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2484 513 Size: 552 939 480 3238 Fragments arranged by size: 3238 939 552 480 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4470 Fragments arranged by size: 4470 683 56 BtsI GCAGTG_nn' Cuts at: 2164 2725 3519 3539 2164 Size: 561 794 20 3834 Fragments arranged by size: 3834 794 561 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2404 2439 529 Size: 35 1840 35 3299 Fragments arranged by size: 3299 1840 35 35 DraI TTT'AAA Cuts at: 2513 2513 Size: 5209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4216 809 Size: 507 2900 1802 Fragments arranged by size: 2900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 2656 3545 Size: 54 1425 50 275 18 670 156 889 Cuts at: 3545 5174 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 670 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2656 1537 Size: 275 844 4090 Fragments arranged by size: 4090 844 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3139 1353 Size: 1786 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2383 3796 4170 4316 387 Size: 1064 138 794 1413 374 146 1280 Fragments arranged by size: 1413 1280 1064 794 374 146 138 EcoICRI GAG'CTC Cuts at: 2481 2481 Size: 5209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2190 2279 2320 2424 2454 3272 Size: 393 523 35 89 41 104 30 818 Cuts at: 3272 3869 4656 4924 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 818 787 597 523 393 268 104 89 41 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 2472 2472 Size: 5209 EcoRI G'AATT_C Cuts at: 2518 2896 2518 Size: 378 4831 FspI TGC'GCA Cuts at: 3715 3715 Size: 5209 HaeII r_GCGC'y Cuts at: 1678 4356 1678 Size: 2678 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2452 2484 3850 Size: 32 135 32 812 32 36 32 1366 Cuts at: 3850 3882 3924 3956 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1366 812 135 42 36 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 2799 669 Size: 988 281 615 246 3079 Fragments arranged by size: 3079 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5209 HpaI GTT'AAC Cuts at: 2799 2799 Size: 5209 KpnI G_GTAC'C Cuts at: 2534 2534 Size: 5209 MfeI C'AATT_G Cuts at: 2323 2808 2323 Size: 485 4724 MluI A'CGCG_T Cuts at: 5085 5085 Size: 5209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4323 4507 1305 Size: 3018 184 2007 Fragments arranged by size: 3018 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3387 Fragments arranged by size: 3387 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2139 3167 3526 3685 518 Size: 648 963 10 1028 359 159 2042 Fragments arranged by size: 2042 1028 963 648 359 159 10 MspA1I CmG'CkG Cuts at: 1606 2175 2625 3302 4450 4695 1606 Size: 569 450 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 569 450 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3718 Fragments arranged by size: 3718 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5209 NotI GC'GGCC_GC Cuts at: 2656 2656 Size: 5209 NspI r_CATG'y Cuts at: 1132 1229 2567 4112 1132 Size: 97 1338 1545 2229 Fragments arranged by size: 2229 1545 1338 97 PasI CC'CwG_GG Cuts at: 1348 2275 1348 Size: 927 4282 PciI A'CATG_T Cuts at: 4108 4108 Size: 5209 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5209 PfoI T'CCnGG_A Cuts at: 2445 2470 2445 Size: 25 5184 PmeI GTTT'AAAC Cuts at: 2513 2513 Size: 5209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2273 2305 3278 3310 4791 4823 2273 Size: 32 973 32 1481 32 2659 Fragments arranged by size: 2659 1481 973 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5209 PsiI TTA'TAA Cuts at: 2779 2779 Size: 5209 PstI C_TGCA'G Cuts at: 830 2561 830 Size: 1731 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5209 PvuII CAG'CTG Cuts at: 2175 2175 Size: 5209 SacI G_AGCT'C Cuts at: 2483 2483 Size: 5209 SalI G'TCGA_C Cuts at: 1936 2551 1936 Size: 615 4594 SbfI CC_TGCA'GG Cuts at: 2561 2561 Size: 5209 ScaI AGT'ACT Cuts at: 3457 5169 3457 Size: 1712 3497 SfcI C'TryA_G Cuts at: 826 1045 2557 3692 4373 4564 826 Size: 219 1512 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5209 SmaI CCC'GGG Cuts at: 1872 2184 2536 1872 Size: 312 352 4545 Fragments arranged by size: 4545 352 312 SmlI C'TyrA_G Cuts at: 820 1017 1943 3313 4214 4476 820 Size: 197 926 1370 901 262 1553 Fragments arranged by size: 1553 1370 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3000 493 Size: 2507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5209 SphI G_CATG'C Cuts at: 1132 2567 1132 Size: 1435 3774 SspI AAT'ATT Cuts at: 5 52 3133 5 Size: 47 3081 2081 Fragments arranged by size: 3081 2081 47 StuI AGG'CCT Cuts at: 1517 2061 2303 1517 Size: 544 242 4423 Fragments arranged by size: 4423 544 242 StyI C'CwwG_G Cuts at: 513 1065 2004 2648 513 Size: 552 939 644 3074 Fragments arranged by size: 3074 939 644 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3236 3253 3406 3591 4036 325 Size: 1255 1656 17 153 185 445 1498 Fragments arranged by size: 1656 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2231 3455 5167 Size: 274 80 33 51 1443 254 1224 1712 Cuts at: 5167 96 Size: 138 Fragments arranged by size: 1712 1443 1224 274 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5169 5201 5169 Size: 32 5177 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5209 XbaI T'CTAG_A Cuts at: 2545 2545 Size: 5209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5209 XmaI C'CCGG_G Cuts at: 1870 2182 2534 1870 Size: 312 352 4545 Fragments arranged by size: 4545 352 312 XmnI GAAnn'nnTTC Cuts at: 2253 3338 2253 Size: 1085 4124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3017 276 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI