Restriction map for
pFN21AE0508
(Linear) (Six-base) MAPSORT of: KIEE0508.seq Check: 8689 from: 1 to: 504
KIEE0508.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 419 504
Size: 315 104 85
Fragments arranged by size:
315 104 85
AflIII A'CryG_T
Cuts at: 0 35 504
Size: 35 469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 504
Size: 268 32 204
Fragments arranged by size:
268 204 32
AlwNI CAG_nnn'CTG
Cuts at: 0 208 354 504
Size: 208 146 150
Fragments arranged by size:
208 150 146
ApaLI G'TGCA_C
Cuts at: 0 127 504
Size: 127 377
AvaI C'yCGr_G
Cuts at: 0 177 504
Size: 177 327
BanII G_rGCy'C
Cuts at: 0 478 504
Size: 478 26
BlpI GC'TnA_GC
Cuts at: 0 70 504
Size: 70 434
Bme1580I G_kGCm'C
Cuts at: 0 131 504
Size: 131 373
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 504
Size: 368 136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 279 504
Size: 279 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 185 274 449 504
Size: 150 35 89 175 55
Fragments arranged by size:
175 150 89 55 35
BsaAI yAC'GTr
Cuts at: 0 36 504
Size: 36 468
BsaWI w'CCGG_w
Cuts at: 0 21 504
Size: 21 483
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 268 280 298 504
Size: 250 18 12 18 206
Fragments arranged by size:
250 206 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 255 504
Size: 255 249
BseYI C'CCAG_C
Cuts at: 0 84 504
Size: 84 420
BsiHKAI G_wGCw'C
Cuts at: 0 131 478 504
Size: 131 347 26
Fragments arranged by size:
347 131 26
Bsp1286I G_dGCh'C
Cuts at: 0 131 478 504
Size: 131 347 26
Fragments arranged by size:
347 131 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 504
Size: 398 106
BsrDI GCAATG_nn'
Cuts at: 0 187 504
Size: 187 317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 84 504
Size: 57 27 420
Fragments arranged by size:
420 57 27
BstYI r'GATC_y
Cuts at: 0 455 504
Size: 455 49
BtgI C'CryG_G
Cuts at: 0 479 504
Size: 479 25
BtsI GCAGTG_nn'
Cuts at: 0 159 504
Size: 159 345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 504
Size: 399 35 70
Fragments arranged by size:
399 70 35
EaeI y'GGCC_r
Cuts at: 0 495 504
Size: 495 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 378 504
Size: 378 126
EcoICRI GAG'CTC
Cuts at: 0 476 504
Size: 476 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 185 274 315 419 449 504
Size: 150 35 89 41 104 30 55
Fragments arranged by size:
150 104 89 55 41 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 467 504
Size: 467 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 447 479 504
Size: 379 32 36 32 25
Fragments arranged by size:
379 36 32 32 25
MfeI C'AATT_G
Cuts at: 0 318 504
Size: 318 186
MslI CAynn'nnrTG
Cuts at: 0 124 134 504
Size: 124 10 370
Fragments arranged by size:
370 124 10
MspA1I CmG'CkG
Cuts at: 0 170 504
Size: 170 334
PasI CC'CwG_GG
Cuts at: 0 270 504
Size: 270 234
PfoI T'CCnGG_A
Cuts at: 0 440 465 504
Size: 440 25 39
Fragments arranged by size:
440 39 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 504
Size: 268 32 204
Fragments arranged by size:
268 204 32
PvuII CAG'CTG
Cuts at: 0 170 504
Size: 170 334
SacI G_AGCT'C
Cuts at: 0 478 504
Size: 478 26
SmaI CCC'GGG
Cuts at: 0 179 504
Size: 179 325
StuI AGG'CCT
Cuts at: 0 56 298 504
Size: 56 242 206
Fragments arranged by size:
242 206 56
TatI w'GTAC_w
Cuts at: 0 226 504
Size: 226 278
XmaI C'CCGG_G
Cuts at: 0 177 504
Size: 177 327
XmnI GAAnn'nnTTC
Cuts at: 0 248 504
Size: 248 256
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaLI AvaI BanII BlpI
Bme1580I BmrI BplI BpmI BsaAI BsaWI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrDI BstXI BstYI BtgI
BtsI CspCI EaeI EciI EcoICRI Eco57MI EcoNI Hin4I
MfeI MslI MspA1I PasI PfoI PpiI PvuII SacI
SmaI StuI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EarI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI ZraI