(Circular) (Six-base) MAPSORT of: pFN21AE0365.seq Check: 3654 from: 1 to: 6265 pFN21AE0365 6265 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 2504 2611 3110 3179 4328 5712 5980 Size: 776 96 107 499 69 1149 1384 268 Cuts at: 5980 1632 Size: 1917 Fragments arranged by size: 1917 1384 1149 776 499 268 107 96 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2807 1184 Size: 1623 4642 AlwNI CAG_nnn'CTG Cuts at: 2565 2781 2969 3314 3405 4008 5580 2565 Size: 216 188 345 91 603 1572 3250 Fragments arranged by size: 3250 1572 603 345 216 188 91 ApaI G_GGCC'C Cuts at: 2285 3399 2285 Size: 1114 5151 ApaLI G'TGCA_C Cuts at: 2792 4322 5478 2792 Size: 1530 1156 3579 Fragments arranged by size: 3579 1530 1156 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2826 3042 3586 5041 618 Size: 325 217 627 1039 216 544 1455 1842 Fragments arranged by size: 1842 1455 1039 627 544 325 217 216 BanII G_rGCy'C Cuts at: 1391 2285 2412 2688 3399 3584 1391 Size: 894 127 276 711 185 4072 Fragments arranged by size: 4072 894 711 276 185 127 BbeI G_GCGC'C Cuts at: 2830 2830 Size: 6265 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 928 Size: 971 466 4828 Fragments arranged by size: 4828 971 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BfrBI ATG'CAT Cuts at: 2527 2622 2527 Size: 95 6170 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 2015 2915 3682 2015 Size: 900 767 4598 Fragments arranged by size: 4598 900 767 Bme1580I G_kGCm'C Cuts at: 1790 2285 2695 2796 3399 4326 5482 1790 Size: 495 410 101 603 927 1156 2573 Fragments arranged by size: 2573 1156 927 603 495 410 101 BmgBI CAC'GTC Cuts at: 1318 3220 1318 Size: 1902 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 3127 3996 Size: 416 53 83 399 24 1235 475 869 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1235 958 869 475 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4925 1239 Size: 3686 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2245 4390 5255 5553 2245 Size: 2145 865 298 2957 Fragments arranged by size: 2957 2145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2459 2827 3506 4072 Size: 53 83 186 936 926 368 679 566 Cuts at: 4072 4454 5089 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 926 679 635 566 382 368 186 83 53 BsaWI w'CCGG_w Cuts at: 2148 2724 4698 5096 5370 5517 5928 2148 Size: 576 1974 398 274 147 411 2485 Fragments arranged by size: 2485 1974 576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6040 Fragments arranged by size: 6040 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 1186 Size: 1045 5220 BseYI C'CCAG_C Cuts at: 2029 2238 2398 5468 5964 2029 Size: 209 160 3070 496 2330 Fragments arranged by size: 3070 2330 496 209 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3193 1163 Size: 573 1457 4235 Fragments arranged by size: 4235 1457 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 3715 4476 5504 1540 Size: 275 186 139 1575 761 1028 2301 Fragments arranged by size: 2301 1575 1028 761 275 186 139 BsiHKAI G_wGCw'C Cuts at: 2586 2688 2796 2910 3228 4326 4411 5482 2586 Size: 102 108 114 318 1098 85 1071 3369 Fragments arranged by size: 3369 1098 1071 318 114 108 102 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2412 2586 2688 2695 2796 2910 Size: 399 495 127 174 102 7 101 114 Cuts at: 2910 3228 3399 3584 4326 4411 5482 1391 Size: 318 171 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 318 185 174 171 127 114 102 101 85 7 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2824 4152 1394 Size: 429 1001 1328 3507 Fragments arranged by size: 3507 1328 1001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 3066 4909 5096 1700 Size: 110 231 1025 1843 187 2869 Fragments arranged by size: 2869 1843 1025 231 187 110 BsrGI T'GTAC_A Cuts at: 96 3027 96 Size: 2931 3334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 3076 794 Size: 2282 3983 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 2189 3363 1184 Size: 1005 1174 4086 Fragments arranged by size: 4086 1174 1005 BstXI CCAn_nnnn'nTGG Cuts at: 2890 2890 Size: 6265 BstYI r'GATC_y Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260 Size: 1623 367 385 17 1452 86 12 346 Cuts at: 6260 1972 Size: 1977 Fragments arranged by size: 1977 1623 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2036 3315 2036 Size: 1279 4986 BtgI C'CryG_G Cuts at: 513 1065 2057 2550 3000 3202 513 Size: 552 992 493 450 202 3576 Fragments arranged by size: 3576 992 552 493 450 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3440 487 Size: 683 56 2214 3312 Fragments arranged by size: 3312 2214 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2445 3239 3317 5272 809 Size: 507 1129 794 78 1955 1802 Fragments arranged by size: 1955 1802 1129 794 507 78 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2247 2547 Size: 54 1425 50 275 18 307 110 300 Cuts at: 2547 3003 3305 3712 4601 6230 8 Size: 456 302 407 889 1629 43 Fragments arranged by size: 1629 1425 889 456 407 307 302 300 275 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2137 3712 1537 Size: 275 325 1575 4090 Fragments arranged by size: 4090 1575 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3353 4195 1353 Size: 2000 842 3423 Fragments arranged by size: 3423 2000 842 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2686 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2504 2611 3110 3179 4328 4925 Size: 393 776 96 107 499 69 1149 597 Cuts at: 4925 5712 5980 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1149 787 776 597 499 393 268 107 96 69 EcoO109I rG'GnC_Cy Cuts at: 2082 2230 2282 2778 3395 2082 Size: 148 52 496 617 4952 Fragments arranged by size: 4952 617 496 148 52 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2770 2830 3455 5412 1678 Size: 1092 60 625 1957 2531 Fragments arranged by size: 2531 1957 1092 625 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2319 2351 4906 4938 4980 Size: 32 135 32 747 32 2555 32 42 Cuts at: 4980 5012 1373 Size: 32 2626 Fragments arranged by size: 2626 2555 747 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KasI G'GCGC_C Cuts at: 2826 2826 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 2641 3864 2641 Size: 1223 5042 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 2549 3307 10 Size: 54 1425 343 417 300 758 2968 Fragments arranged by size: 2968 1425 758 417 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2056 2978 4223 4582 4741 518 Size: 648 890 922 1245 359 159 2042 Fragments arranged by size: 2042 1245 922 890 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2242 3204 3681 4358 5506 5751 1606 Size: 636 962 477 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 636 477 245 NaeI GCC'GGC Cuts at: 3068 3068 Size: 6265 NarI GG'CG_CC Cuts at: 2827 2827 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2550 513 Size: 552 1485 4228 Fragments arranged by size: 4228 1485 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 3066 3066 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NruI TCG'CGA Cuts at: 3038 3425 3038 Size: 387 5878 NsiI A_TGCA'T Cuts at: 2529 2624 2529 Size: 95 6170 NspI r_CATG'y Cuts at: 1132 1229 2531 3623 5168 1132 Size: 97 1302 1092 1545 2229 Fragments arranged by size: 2229 1545 1302 1092 97 PasI CC'CwG_GG Cuts at: 1348 2260 3559 1348 Size: 912 1299 4054 Fragments arranged by size: 4054 1299 912 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 3220 1108 Size: 1137 975 4153 Fragments arranged by size: 4153 1137 975 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2230 Size: 6265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2281 3395 2281 Size: 1114 5151 PstI C_TGCA'G Cuts at: 830 2616 2786 3617 830 Size: 1786 170 831 3478 Fragments arranged by size: 3478 1786 831 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2242 2242 Size: 6265 SacI G_AGCT'C Cuts at: 2688 2688 Size: 6265 SacII CC_GC'GG Cuts at: 3205 3205 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2612 2782 3613 4748 5429 5620 826 Size: 219 1567 170 831 1135 681 191 1471 Fragments arranged by size: 1567 1471 1135 831 681 219 191 170 SfoI GGC'GCC Cuts at: 2828 2828 Size: 6265 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2224 4369 5270 5532 820 Size: 197 926 281 2145 901 262 1553 Fragments arranged by size: 2145 1553 926 901 281 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 2531 3623 1132 Size: 1399 1092 3774 Fragments arranged by size: 3774 1399 1092 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2550 2871 3704 513 Size: 552 1485 321 833 3074 Fragments arranged by size: 3074 1485 833 552 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3060 3227 4292 4309 4462 4647 5092 Size: 1255 1480 167 1065 17 153 185 445 Cuts at: 5092 325 Size: 1498 Fragments arranged by size: 1498 1480 1255 1065 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 3027 4511 Size: 274 80 33 51 1443 765 285 1484 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1484 1443 765 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 2321 2369 1316 Size: 1005 48 5212 Fragments arranged by size: 5212 1005 48 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 2180 4394 2180 Size: 2214 4051 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI