Restriction map for
pFN21AE0377
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbsI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspEI
BspLU11I BsrBI Bst1107I BstAPI BstBI
BstZ17I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I FbaI FseI HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Aor51HI 188 AGCGCT AGC^GCT
ApaI 220 GGGCCC GGGCC^C
Asp700I 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1223 CCCGGG C^[CT]CG[GA]G
AviII 605 TGCGCA TGC^GCA
BalI 1331 TGGCCA TGG^CCA
BamHI 777 GGATCC G^GATCC
BanI 493 GGCGCC G^G[CT][GA]CC
BbeI 493 GGCGCC GGCGC^C
BbrPI 1429 CACGTG CAC^GTG
BbvCI 283 GCTGAGG CCTCAGC(-5/-2)
BcgI 1371 GCAGAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 730 CTGGAG CTGGAG(16/14)
BsaAI 1429 CACGTG [CT]AC^GT[GA]
BsoBI 1223 CCCGGG C^[CT]CG[GA]G
Bsp1407I 161 TGTACA T^GTACA
BspDI 502 ATCGAT AT^CGAT
BsrDI 116 CATTGC GCAATG(2/0)
BsrFI 1013 ACCGGC [GA]^CCGG[CT]
BsrGI 161 TGTACA T^GTACA
BssHII 366 GCGCGC G^CGCGC
BssSI 1069 CACGAG CACGAG(-5/-1)
BstEII 24 GGTGACC G^GT[ACGT]ACC
BstPI 24 GGTGACC G^GT[ACGT]ACC
Cfr10I 1013 ACCGGC [GA]^CCGG[CT]
ClaI 502 ATCGAT AT^CGAT
EaeI 1331 TGGCCA [CT]^GGCC[GA]
Eco47III 188 AGCGCT AGC^GCT
EcoNI 1152 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 24 GGTGACC G^GT[ACGT]ACC
EcoRV 1408 GATATC GAT^ATC
FspI 605 TGCGCA TGC^GCA
HgaI 472 GACGC GACGC(5/10)
HincII 1120 GTCAAC GT[CT]^[GA]AC
HindII 1120 GTCAAC GT[CT]^[GA]AC
HindIII 621 AAGCTT A^AGCTT
HinfI 805 GACTC G^A[ACGT]TC
KasI 493 GGCGCC G^GCGCC
MluNI 1331 TGGCCA TGG^CCA
MscI 1331 TGGCCA TGG^CCA
NarI 493 GGCGCC GG^CGCC
NcoI 1100 CCATGG C^CATGG
PleI 805 GACTC GAGTC(4/5)
PmaCI 1429 CACGTG CAC^GTG
PmlI 1429 CACGTG CAC^GTG
PpuMI 105 GGGACCC [GA]G^G[AT]CC[CT]
PstI 139 CTGCAG CTGCA^G
PvuII 142 CAGCTG CAG^CTG
SacI 1342 GAGCTC GAGCT^C
SexAI 869 ACCTGGT A^CC[AT]GGT
SfcI 139 CTGCAG C^T[GA][CT]AG
SfoI 493 GGCGCC GGC^GCC
SmaI 1223 CCCGGG CCC^GGG
SphI 821 GCATGC GCATG^C
SspBI 161 TGTACA T^GTACA
Tsp509I 784 AATT ^AATT
XmaCI 1223 CCCGGG C^CCGGG
XmaI 1223 CCCGGG C^CCGGG
XmnI 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 367 CGCG CG^CG
427 CGCG
AcyI 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
AfaI 162 GTAC GT^AC
1311 GTAC
AflIII 1415 ACGTGT A^C[GA][CT]GT
1428 ACACGT
BsaHI 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
BsaI 910 GAGACC GGTCTC(1/5)
1199 GAGACC
BspHI 173 TCATGA T^CATGA
950 TCATGA
BspMI 137 ACCTGC ACCTGC(4/8)
686 ACCTGC
BsrI 323 ACTGG ACTGG(1/-1)
627 CCAGT
BstF5I 444 CATCC GGATG(2/0)
816 CATCC
BstUI 367 CGCG CG^CG
427 CGCG
BstYI 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
EcoT14I 974 CCAAGG C^C[AT][AT]GG
1100 CCATGG
FokI 444 CATCC GGATG(9/13)
816 CATCC
Hin1I 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
MaeII 1415 ACGT A^CGT
1430 ACGT
MaeIII 25 GTGAC ^GT[ACGT]AC
597 GTCAC
MflI 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
MseI 713 TTAA T^TAA
1268 TTAA
MspA1I 142 CAGCTG C[AC]G^C[GT]G
294 CAGCGG
MvnI 367 CGCG CG^CG
427 CGCG
NspI 821 GCATGC [GA]CATG^[CT]
1091 ACATGC
RcaI 173 TCATGA T^CATGA
950 TCATGA
RsaI 162 GTAC GT^AC
1311 GTAC
SapI 81 GAAGAGC GCTCTTC(1/4)
327 GCTCTTC
StuI 707 AGGCCT AGG^CCT
1326 AGGCCT
StyI 974 CCAAGG C^C[AT][AT]GG
1100 CCATGG
Tru9I 713 TTAA T^TAA
1268 TTAA
Tsp45I 25 GTGAC ^GT[GC]AC
597 GTCAC
XhoII 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AocI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstNI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I