(Circular) (Six-base) MAPSORT of: pFN21AE0377.seq Check: 4945 from: 1 to: 6187 pFN21AE0377 6187 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2700 2700 Size: 6187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3192 4250 5634 5902 1632 Size: 1560 1058 1384 268 1917 Fragments arranged by size: 1917 1560 1384 1058 268 AfeI AGC'GCT Cuts at: 2195 2195 Size: 6187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 3420 3433 5086 6063 1203 Size: 2217 13 1653 977 1327 Fragments arranged by size: 2217 1653 1327 977 13 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 3173 1166 Size: 2007 4180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2948 1184 Size: 1764 4423 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 6155 AlwNI CAG_nnn'CTG Cuts at: 3930 5502 3930 Size: 1572 4615 ApaI G_GGCC'C Cuts at: 2229 2229 Size: 6187 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 3496 3722 3874 5983 1493 Size: 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3228 3512 1870 Size: 73 1285 284 4545 Fragments arranged by size: 4545 1285 284 73 BamHI G'GATC_C Cuts at: 2782 3517 3884 2782 Size: 735 367 5085 Fragments arranged by size: 5085 735 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2498 3508 4963 618 Size: 325 217 627 711 1010 1455 1842 Fragments arranged by size: 1842 1455 1010 711 627 325 217 BanII G_rGCy'C Cuts at: 1391 2229 3194 3351 3506 1391 Size: 838 965 157 155 4072 Fragments arranged by size: 4072 965 838 157 155 BbeI G_GCGC'C Cuts at: 2502 2502 Size: 6187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5216 BbvCI CC'TCA_GC Cuts at: 2289 2289 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3365 3399 4378 4412 1903 Size: 34 50 34 1344 34 979 34 3678 Fragments arranged by size: 3678 1344 979 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4798 136 Size: 107 122 71 631 605 337 2789 1525 Fragments arranged by size: 2789 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2229 2492 2912 3170 3297 4248 5404 1790 Size: 439 263 420 258 127 951 1156 2573 Fragments arranged by size: 2573 1156 951 439 420 263 258 127 BmgBI CAC'GTC Cuts at: 1318 3421 1318 Size: 2103 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2689 3116 2689 Size: 427 5760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 4847 1239 Size: 1517 2091 2579 Fragments arranged by size: 2579 2091 1517 Bpu10I CC'TnA_GC Cuts at: 2289 2525 2289 Size: 236 5951 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2909 3198 4850 882 Size: 617 1410 289 1652 2219 Fragments arranged by size: 2219 1652 1410 617 289 BsaAI yAC'GTr Cuts at: 493 1204 3436 3978 493 Size: 711 2232 542 2702 Fragments arranged by size: 2702 2232 711 542 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2478 2499 3994 4376 Size: 53 83 186 936 945 21 1495 382 Cuts at: 4376 5011 275 Size: 635 1451 Fragments arranged by size: 1495 1451 945 936 635 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5962 Fragments arranged by size: 5962 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2471 2849 2852 3005 1186 Size: 1120 165 378 3 153 4368 Fragments arranged by size: 4368 1120 378 165 153 3 BseYI C'CCAG_C Cuts at: 2620 3187 3243 5390 5886 2620 Size: 567 56 2147 496 2921 Fragments arranged by size: 2921 2147 567 496 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5614 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2766 2927 3351 4248 4333 5404 2766 Size: 161 424 897 85 1071 3549 Fragments arranged by size: 3549 1071 897 424 161 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2492 2766 2912 2927 3170 3194 Size: 399 439 263 274 146 15 243 24 Cuts at: 3194 3297 3351 3506 4248 4333 5404 1391 Size: 103 54 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 274 263 243 155 146 103 85 54 24 15 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 2178 2955 4076 5806 2178 Size: 777 1121 1730 2559 Fragments arranged by size: 2559 1730 1121 777 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2700 3542 844 Size: 1307 549 842 3489 Fragments arranged by size: 3489 1307 842 549 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2120 4682 4856 65 Size: 1183 872 2562 174 1396 Fragments arranged by size: 2562 1396 1183 872 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3018 4831 5018 1700 Size: 110 1208 1813 187 2869 Fragments arranged by size: 2869 1813 1208 187 110 BsrGI T'GTAC_A Cuts at: 96 2166 96 Size: 2070 4117 BssHII G'CGCG_C Cuts at: 1925 2371 1925 Size: 446 5741 BssSI C'ACGA_G Cuts at: 3074 4247 5259 3074 Size: 1173 1012 4002 Fragments arranged by size: 4002 1173 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 2029 1184 Size: 845 5342 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2758 3089 3112 3436 2221 Size: 537 331 23 324 4972 Fragments arranged by size: 4972 537 331 324 23 BstYI r'GATC_y Cuts at: 1972 2782 3078 3517 3884 4269 4286 5738 5824 Size: 810 296 439 367 385 17 1452 86 Cuts at: 5824 5836 6182 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 810 439 385 367 346 296 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2014 2408 3162 3408 2014 Size: 394 754 246 4793 Fragments arranged by size: 4793 754 394 246 BtgI C'CryG_G Cuts at: 513 1065 2649 2795 3105 513 Size: 552 1584 146 310 3595 Fragments arranged by size: 3595 1584 552 310 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2383 2731 487 Size: 683 56 1157 348 3943 Fragments arranged by size: 3943 1157 683 348 56 BtsI GCAGTG_nn' Cuts at: 3072 3703 4497 4517 3072 Size: 631 794 20 4742 Fragments arranged by size: 4742 794 631 20 ClaI AT'CG_AT Cuts at: 1554 2508 1554 Size: 954 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3336 3634 4523 Size: 54 1425 50 275 18 1506 298 889 Cuts at: 4523 6152 8 Size: 1629 43 Fragments arranged by size: 1629 1506 1425 889 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2339 2680 2903 3272 3362 4117 1353 Size: 728 258 341 223 369 90 755 3423 Fragments arranged by size: 3423 755 728 369 341 258 223 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3349 3349 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2756 3192 4250 4847 5634 5902 1239 Size: 393 1124 436 1058 597 787 268 1524 Fragments arranged by size: 1524 1124 1058 787 597 436 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3161 3161 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 2111 2225 2226 2111 Size: 114 1 6072 Fragments arranged by size: 6072 114 1 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 EcoRV GAT'ATC Cuts at: 3415 3415 Size: 6187 FspI TGC'GCA Cuts at: 2612 4693 2612 Size: 2081 4106 HaeII r_GCGC'y Cuts at: 1678 2197 2392 2502 5334 1678 Size: 519 195 110 2832 2531 Fragments arranged by size: 2832 2531 519 195 110 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 4828 4860 4902 Size: 32 135 32 1013 32 2211 32 42 Cuts at: 4902 4934 1373 Size: 32 2626 Fragments arranged by size: 2626 2211 1013 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3127 3531 3777 669 Size: 988 281 1189 404 246 3079 Fragments arranged by size: 3079 1189 988 404 281 246 HindIII A'AGCT_T Cuts at: 748 2626 748 Size: 1878 4309 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KasI G'GCGC_C Cuts at: 2498 2498 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3246 5301 5485 1305 Size: 1941 2055 184 2007 Fragments arranged by size: 2055 2007 1941 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3338 10 Size: 54 1425 343 1506 2859 Fragments arranged by size: 2859 1506 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2825 3173 3371 4145 4504 4663 518 Size: 648 1659 348 198 774 359 159 2042 Fragments arranged by size: 2042 1659 774 648 359 348 198 159 MspA1I CmG'CkG Cuts at: 1606 2149 2301 3603 4280 5428 5673 1606 Size: 543 152 1302 677 1148 245 2120 Fragments arranged by size: 2120 1302 1148 677 543 245 152 NarI GG'CG_CC Cuts at: 2499 2499 Size: 6187 NcoI C'CATG_G Cuts at: 513 1065 3105 513 Size: 552 2040 3595 Fragments arranged by size: 3595 2040 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2830 3100 3545 5090 1132 Size: 97 1601 270 445 1545 2229 Fragments arranged by size: 2229 1601 1545 445 270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2107 2451 3252 2107 Size: 344 801 5042 Fragments arranged by size: 5042 801 344 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PmlI CAC'GTG Cuts at: 3436 3436 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2111 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PspOMI G'GGCC_C Cuts at: 2225 2225 Size: 6187 PstI C_TGCA'G Cuts at: 830 2148 3539 830 Size: 1318 1391 3478 Fragments arranged by size: 3478 1391 1318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2149 2149 Size: 6187 SacI G_AGCT'C Cuts at: 3351 3351 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 2111 2111 Size: 6187 SapI GCTCTTCn'nnn_ Cuts at: 2081 2339 2081 Size: 258 5929 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SexAI A'CCwGG_T Cuts at: 2874 2874 Size: 6187 SfcI C'TryA_G Cuts at: 826 1045 2144 3535 4670 5351 5542 826 Size: 219 1099 1391 1135 681 191 1471 Fragments arranged by size: 1471 1391 1135 1099 681 219 191 SfoI GGC'GCC Cuts at: 2500 2500 Size: 6187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3230 3514 1872 Size: 1358 284 4545 Fragments arranged by size: 4545 1358 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 2830 3545 1132 Size: 1698 715 3774 Fragments arranged by size: 3774 1698 715 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 2714 3333 1517 Size: 1197 619 4371 Fragments arranged by size: 4371 1197 619 StyI C'CwwG_G Cuts at: 513 1065 2979 3105 3626 513 Size: 552 1914 126 521 3074 Fragments arranged by size: 3074 1914 552 521 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2979 4214 4231 4384 4569 5014 325 Size: 1255 1399 1235 17 153 185 445 1498 Fragments arranged by size: 1498 1399 1255 1235 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 4433 6145 Size: 274 80 33 51 1443 189 2267 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 2267 1712 1443 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2767 2799 6147 6179 2767 Size: 32 3348 32 2775 Fragments arranged by size: 3348 2775 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3228 3512 1870 Size: 1358 284 4545 Fragments arranged by size: 4545 1358 284 XmnI GAAnn'nnTTC Cuts at: 2897 4316 2897 Size: 1419 4768 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI XcmI