Restriction map for
pFN21AE0377
(Linear) (Six-base) MAPSORT of: KIEE0377.seq Check: 3425 from: 1 to: 1482
KIEE0377.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 695 1482
Size: 695 787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 1482
Size: 1187 295
AfeI AGC'GCT
Cuts at: 0 190 1482
Size: 190 1292
AflIII A'CryG_T
Cuts at: 0 1415 1428 1482
Size: 1415 13 54
Fragments arranged by size:
1415 54 13
AleI CACnn'nnGTG
Cuts at: 0 1168 1482
Size: 1168 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1482
Size: 943 539
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1482
Size: 574 32 876
Fragments arranged by size:
876 574 32
ApaI G_GGCC'C
Cuts at: 0 224 1482
Size: 224 1258
AvaI C'yCGr_G
Cuts at: 0 1223 1482
Size: 1223 259
BamHI G'GATC_C
Cuts at: 0 777 1482
Size: 777 705
BanI G'GyrC_C
Cuts at: 0 493 1482
Size: 493 989
BanII G_rGCy'C
Cuts at: 0 224 1189 1346 1482
Size: 224 965 157 136
Fragments arranged by size:
965 224 157 136
BbeI G_GCGC'C
Cuts at: 0 497 1482
Size: 497 985
BbvCI CC'TCA_GC
Cuts at: 0 284 1482
Size: 284 1198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1360 1394 1482
Size: 1360 34 88
Fragments arranged by size:
1360 88 34
Bme1580I G_kGCm'C
Cuts at: 0 224 487 907 1165 1292 1482
Size: 224 263 420 258 127 190
Fragments arranged by size:
420 263 258 224 190 127
BmgBI CAC'GTC
Cuts at: 0 1416 1482
Size: 1416 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 684 1111 1482
Size: 684 427 371
Fragments arranged by size:
684 427 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1482
Size: 751 731
Bpu10I CC'TnA_GC
Cuts at: 0 284 520 1482
Size: 284 236 962
Fragments arranged by size:
962 284 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 904 1193 1482
Size: 904 289 289
Fragments arranged by size:
904 289 289
BsaAI yAC'GTr
Cuts at: 0 1431 1482
Size: 1431 51
BsaHI Gr'CG_yC
Cuts at: 0 473 494 1482
Size: 473 21 988
Fragments arranged by size:
988 473 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 466 844 847 1000 1482
Size: 301 165 378 3 153 482
Fragments arranged by size:
482 378 301 165 153 3
BseYI C'CCAG_C
Cuts at: 0 615 1182 1238 1482
Size: 615 567 56 244
Fragments arranged by size:
615 567 244 56
BsiHKAI G_wGCw'C
Cuts at: 0 761 922 1346 1482
Size: 761 161 424 136
Fragments arranged by size:
761 424 161 136
Bsp1286I G_dGCh'C
Cuts at: 0 224 487 761 907 922 1165 1189 1292
Size: 224 263 274 146 15 243 24 103
Cuts at: 1292 1346 1482
Size: 54 136
Fragments arranged by size:
274 263 243 224 146 136 103 54
24 15
BspHI T'CATG_A
Cuts at: 0 173 950 1482
Size: 173 777 532
Fragments arranged by size:
777 532 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 695 1482
Size: 146 549 787
Fragments arranged by size:
787 549 146
BsrDI GCAATG_nn'
Cuts at: 0 115 1482
Size: 115 1367
BsrFI r'CCGG_y
Cuts at: 0 1013 1482
Size: 1013 469
BsrGI T'GTAC_A
Cuts at: 0 161 1482
Size: 161 1321
BssHII G'CGCG_C
Cuts at: 0 366 1482
Size: 366 1116
BssSI C'ACGA_G
Cuts at: 0 1069 1482
Size: 1069 413
BstEII G'GTnAC_C
Cuts at: 0 24 1482
Size: 24 1458
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 753 1084 1107 1431 1482
Size: 216 537 331 23 324 51
Fragments arranged by size:
537 331 324 216 51 23
BstYI r'GATC_y
Cuts at: 0 777 1073 1482
Size: 777 296 409
Fragments arranged by size:
777 409 296
Bsu36I CC'TnA_GG
Cuts at: 0 9 403 1157 1403 1482
Size: 9 394 754 246 79
Fragments arranged by size:
754 394 246 79 9
BtgI C'CryG_G
Cuts at: 0 644 790 1100 1482
Size: 644 146 310 382
Fragments arranged by size:
644 382 310 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 726 1482
Size: 378 348 756
Fragments arranged by size:
756 378 348
BtsI GCAGTG_nn'
Cuts at: 0 1067 1482
Size: 1067 415
ClaI AT'CG_AT
Cuts at: 0 503 1482
Size: 503 979
EaeI y'GGCC_r
Cuts at: 0 1331 1482
Size: 1331 151
EarI CTCTTCn'nnn_
Cuts at: 0 76 334 675 898 1267 1357 1482
Size: 76 258 341 223 369 90 125
Fragments arranged by size:
369 341 258 223 125 90 76
EcoICRI GAG'CTC
Cuts at: 0 1344 1482
Size: 1344 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1187 1482
Size: 751 436 295
Fragments arranged by size:
751 436 295
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1156 1482
Size: 1156 326
EcoO109I rG'GnC_Cy
Cuts at: 0 106 220 221 1482
Size: 106 114 1 1261
Fragments arranged by size:
1261 114 106 1
EcoRV GAT'ATC
Cuts at: 0 1410 1482
Size: 1410 72
FspI TGC'GCA
Cuts at: 0 607 1482
Size: 607 875
HaeII r_GCGC'y
Cuts at: 0 192 387 497 1482
Size: 192 195 110 985
Fragments arranged by size:
985 195 192 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1482
Size: 580 32 870
Fragments arranged by size:
870 580 32
HincII GTy'rAC
Cuts at: 0 1122 1482
Size: 1122 360
HindIII A'AGCT_T
Cuts at: 0 621 1482
Size: 621 861
KasI G'GCGC_C
Cuts at: 0 493 1482
Size: 493 989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1241 1482
Size: 1241 241
MscI TGG'CCA
Cuts at: 0 1333 1482
Size: 1333 149
MslI CAynn'nnrTG
Cuts at: 0 820 1168 1366 1482
Size: 820 348 198 116
Fragments arranged by size:
820 348 198 116
MspA1I CmG'CkG
Cuts at: 0 144 296 1482
Size: 144 152 1186
Fragments arranged by size:
1186 152 144
NarI GG'CG_CC
Cuts at: 0 494 1482
Size: 494 988
NcoI C'CATG_G
Cuts at: 0 1100 1482
Size: 1100 382
NspI r_CATG'y
Cuts at: 0 825 1095 1482
Size: 825 270 387
Fragments arranged by size:
825 387 270
PfoI T'CCnGG_A
Cuts at: 0 102 446 1247 1482
Size: 102 344 801 235
Fragments arranged by size:
801 344 235 102
PmlI CAC'GTG
Cuts at: 0 1431 1482
Size: 1431 51
PpuMI rG'GwC_Cy
Cuts at: 0 106 1482
Size: 106 1376
PspOMI G'GGCC_C
Cuts at: 0 220 1482
Size: 220 1262
PstI C_TGCA'G
Cuts at: 0 143 1482
Size: 143 1339
PvuII CAG'CTG
Cuts at: 0 144 1482
Size: 144 1338
SacI G_AGCT'C
Cuts at: 0 1346 1482
Size: 1346 136
SanDI GG'GwC_CC
Cuts at: 0 106 1482
Size: 106 1376
SapI GCTCTTCn'nnn_
Cuts at: 0 76 334 1482
Size: 76 258 1148
Fragments arranged by size:
1148 258 76
SexAI A'CCwGG_T
Cuts at: 0 869 1482
Size: 869 613
SfcI C'TryA_G
Cuts at: 0 139 1482
Size: 139 1343
SfoI GGC'GCC
Cuts at: 0 495 1482
Size: 495 987
SmaI CCC'GGG
Cuts at: 0 1225 1482
Size: 1225 257
SphI G_CATG'C
Cuts at: 0 825 1482
Size: 825 657
StuI AGG'CCT
Cuts at: 0 709 1328 1482
Size: 709 619 154
Fragments arranged by size:
709 619 154
StyI C'CwwG_G
Cuts at: 0 974 1100 1482
Size: 974 126 382
Fragments arranged by size:
974 382 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 974 1482
Size: 974 508
TatI w'GTAC_w
Cuts at: 0 161 1482
Size: 161 1321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 762 794 1482
Size: 762 32 688
Fragments arranged by size:
762 688 32
XmaI C'CCGG_G
Cuts at: 0 1223 1482
Size: 1223 259
XmnI GAAnn'nnTTC
Cuts at: 0 892 1482
Size: 892 590
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI ApaI
AvaI BamHI BanI BanII BbeI BbvCI BcgI Bme1580I
BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BseRI
BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FspI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NarI NcoI NspI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI
SfcI SfoI SmaI SphI StuI StyI TaqII TatI
TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BpuEI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BstAPI BstBI BstZ17I CspCI DraI
DraIII DrdI EagI EciI EcoRI FalI FseI FspAI
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
ZraI