Restriction map for
pFN21AB7183
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbsI BcgI BclI BfrI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 559 GACGTC GACGT^C
AccI 1450 GTCTAC GT^[AC][GT]AC
Aor51HI 1277 AGCGCT AGC^GCT
Asp700I 1404 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 253 GGACC G^G[AT]CC
AvrII 94 CCTAGG C^CTAGG
BanI 1358 GGCGCC G^G[CT][GA]CC
BbeI 1358 GGCGCC GGCGC^C
BbvCI 147 GCTGAGG CCTCAGC(-5/-2)
BciVI 809 GTATCC GTATCC(6/5)
BglII 161 AGATCT A^GATCT
BlnI 94 CCTAGG C^CTAGG
BsaI 1111 GAGACC GGTCTC(1/5)
BseRI 1486 CTCCTC GAGGAG(10/8)
BspMI 188 ACCTGC ACCTGC(4/8)
BsrDI 1235 GCAATG GCAATG(2/0)
BstXI 917 CCAACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 1277 AGCGCT AGC^GCT
FauI 1095 GCGGG CCCGC(4/6)
HgaI 975 GACGC GACGC(5/10)
KasI 1358 GGCGCC G^GCGCC
MseI 1505 TTAA T^TAA
MspA1I 1193 CAGCTG C[AC]G^C[GT]G
NarI 1358 GGCGCC GG^CGCC
NspI 1337 GCATGC [GA]CATG^[CT]
PvuII 1193 CAGCTG CAG^CTG
SbfI 323 CCTGCAGG CCTGCA^GG
SfiI 628 GGCCTGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1358 GGCGCC GGC^GCC
SmaI 539 CCCGGG CCC^GGG
SphI 1337 GCATGC GCATG^C
Sse8387I 323 CCTGCAGG CCTGCA^GG
Tru9I 1505 TTAA T^TAA
XmaCI 539 CCCGGG C^CCGGG
XmaI 539 CCCGGG C^CCGGG
XmnI 1404 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 559 GACGTC G[GA]^CG[CT]C
1358 GGCGCC
ApaI 89 GGGCCC GGGCC^C
226 GGGCCC
BalI 491 TGGCCA TGG^CCA
636 TGGCCA
BbrPI 1318 CACGTG CAC^GTG
1396 CACGTG
BcnI 539 CCCGG CC^[GC]GG
540 CCGGG
BsaAI 1318 CACGTG [CT]AC^GT[GA]
1396 CACGTG
BsaHI 559 GACGTC G[GA]^CG[CT]C
1358 GGCGCC
BstAPI 1011 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1392 GCACCACGTGC
BstYI 161 AGATCT [GA]^GATC[CT]
1013 AGATCC
DraIII 1315 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1393 CACCACGTG
Hin1I 559 GACGTC G[GA]^CG[CT]C
1358 GGCGCC
HphI 799 GGTGA GGTGA(8/7)
1155 GGTGA
MaeIII 800 GTGAC ^GT[ACGT]AC
897 GTCAC
MflI 161 AGATCT [GA]^GATC[CT]
1013 AGATCC
MluNI 491 TGGCCA TGG^CCA
636 TGGCCA
MscI 491 TGGCCA TGG^CCA
636 TGGCCA
NciI 539 CCCGG CC^[GC]GG
540 CCGGG
PmaCI 1318 CACGTG CAC^GTG
1396 CACGTG
PmlI 1318 CACGTG CAC^GTG
1396 CACGTG
SacI 874 GAGCTC GAGCT^C
1158 GAGCTC
SmlI 1105 CTCAAG C^T[CT][GA]AG
1430 CTCAAG
Tsp45I 800 GTGAC ^GT[GC]AC
897 GTCAC
XhoII 161 AGATCT [GA]^GATC[CT]
1013 AGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MaeII MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
TspRI Van91I XcmI