(Circular) (Six-base) MAPSORT of: pFN21AB7183.seq Check: 1413 from: 1 to: 6214 pFN21AB7183 6214 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2202 2202 Size: 6214 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2568 4024 278 Size: 53 83 186 936 1032 1456 2468 Fragments arranged by size: 2468 1456 1032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3456 3557 1937 Size: 1519 101 4594 Fragments arranged by size: 4594 1519 101 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 3513 4341 4714 1563 Size: 1950 828 373 3063 Fragments arranged by size: 3063 1950 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2540 3164 3428 4277 5661 5929 1632 Size: 908 624 264 849 1384 268 1917 Fragments arranged by size: 1917 1384 908 849 624 268 264 AfeI AGC'GCT Cuts at: 3284 3284 Size: 6214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 2382 1166 Size: 1216 4998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2047 1184 Size: 863 5351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2678 2710 2678 Size: 32 6182 AlwNI CAG_nnn'CTG Cuts at: 2227 2518 2836 3022 3957 5529 2227 Size: 291 318 186 935 1572 2912 Fragments arranged by size: 2912 1572 935 318 291 186 ApaI G_GGCC'C Cuts at: 2098 2235 2098 Size: 137 6077 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 3523 3749 3901 6010 1493 Size: 2030 226 152 2109 1697 Fragments arranged by size: 2109 2030 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2544 3256 3356 3539 1870 Size: 73 148 453 712 100 183 4545 Fragments arranged by size: 4545 712 453 183 148 100 73 AvrII C'CTAG_G Cuts at: 2099 2099 Size: 6214 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2797 2830 2797 Size: 33 6181 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3363 3535 4990 618 Size: 325 217 627 1576 172 1455 1842 Fragments arranged by size: 1842 1576 1455 627 325 217 172 BanII G_rGCy'C Cuts at: 1391 2098 2235 2349 2883 3167 3305 3533 1391 Size: 707 137 114 534 284 138 228 4072 Fragments arranged by size: 4072 707 534 284 228 138 137 114 BbeI G_GCGC'C Cuts at: 3367 3367 Size: 6214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5243 BbvCI CC'TCA_GC Cuts at: 2153 2153 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2825 4106 5316 2825 Size: 1281 1210 3723 Fragments arranged by size: 3723 1281 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2413 2640 Size: 107 122 71 631 605 421 320 227 Cuts at: 2640 2918 4825 136 Size: 278 1907 1525 Fragments arranged by size: 1907 1525 631 605 421 320 278 227 122 107 71 BglII A'GATC_T Cuts at: 2166 6209 2166 Size: 4043 2171 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2068 2074 2081 2098 2235 2379 3330 4275 Size: 278 6 7 17 137 144 951 945 Cuts at: 4275 5431 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 951 945 278 144 137 17 7 6 BmgBI CAC'GTC Cuts at: 1318 3010 3483 1318 Size: 1692 473 4049 Fragments arranged by size: 4049 1692 473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 2646 3945 Size: 416 53 83 399 24 667 562 1299 Cuts at: 3945 4903 442 Size: 958 1753 Fragments arranged by size: 1753 1299 958 667 562 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2754 2754 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2191 2222 2363 2705 4874 1239 Size: 952 31 141 342 2169 2579 Fragments arranged by size: 2579 2169 952 342 141 31 Bpu10I CC'TnA_GC Cuts at: 2153 3264 2153 Size: 1111 5103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3095 3420 4339 5204 5502 3095 Size: 325 919 865 298 3807 Fragments arranged by size: 3807 919 865 325 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3110 4877 882 Size: 617 1611 1767 2219 Fragments arranged by size: 2219 1767 1611 617 BsaAI yAC'GTr Cuts at: 493 1204 3325 3403 4005 493 Size: 711 2121 78 602 2702 Fragments arranged by size: 2702 2121 711 602 78 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 3364 4021 4403 Size: 53 83 186 936 1032 799 657 382 Cuts at: 4403 5038 275 Size: 635 1451 Fragments arranged by size: 1451 1032 936 799 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2678 2708 2870 2900 1178 Size: 30 165 30 1275 30 162 30 4492 Fragments arranged by size: 4492 1275 165 162 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3482 1186 Size: 2296 3918 BseYI C'CCAG_C Cuts at: 2130 2227 2573 2629 3196 3200 3232 3279 5417 Size: 97 346 56 567 4 32 47 2138 Cuts at: 5417 5913 2130 Size: 496 2431 Fragments arranged by size: 2431 2138 567 496 346 97 56 47 32 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 2486 3032 3380 1163 Size: 573 444 306 546 348 3997 Fragments arranged by size: 3997 573 546 444 348 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2772 2883 3167 4275 4360 5431 2193 Size: 579 111 284 1108 85 1071 2976 Fragments arranged by size: 2976 1108 1071 579 284 111 85 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2074 2081 2098 2193 2235 2349 Size: 399 278 6 7 17 95 42 114 Cuts at: 2349 2379 2772 2883 3167 3305 3330 3533 4275 Size: 30 393 111 284 138 25 203 742 Cuts at: 4275 4360 5431 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 284 278 203 138 114 111 95 85 42 30 25 17 7 6 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 3569 844 Size: 1358 1367 3489 Fragments arranged by size: 3489 1367 1358 BsrBI CCG'CTC Cuts at: 1394 1823 2090 2626 3268 4101 1394 Size: 429 267 536 642 833 3507 Fragments arranged by size: 3507 833 642 536 429 267 BsrDI GCAATG_nn' Cuts at: 65 1248 3247 4709 4883 65 Size: 1183 1999 1462 174 1396 Fragments arranged by size: 1999 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 3022 3403 794 Size: 2228 381 3605 Fragments arranged by size: 3605 2228 381 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 2929 2929 Size: 6214 BstYI r'GATC_y Cuts at: 1972 2166 3018 3544 3911 4296 4313 5765 5851 Size: 194 852 526 367 385 17 1452 86 Cuts at: 5851 5863 6209 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 852 526 385 367 346 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2499 2937 3730 4524 4544 2499 Size: 438 793 794 20 4169 Fragments arranged by size: 4169 794 793 438 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 3325 3403 3325 Size: 78 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 2641 3039 Size: 54 1425 50 275 18 666 145 398 Cuts at: 3039 3661 4550 6179 8 Size: 622 889 1629 43 Fragments arranged by size: 1629 1425 889 666 622 398 275 145 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4144 1353 Size: 2791 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2891 2909 4801 5175 5321 387 Size: 1064 138 1302 18 1892 374 146 1280 Fragments arranged by size: 1892 1302 1280 1064 374 146 138 18 EcoICRI GAG'CTC Cuts at: 2881 3165 2881 Size: 284 5930 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2222 2363 2540 2705 3164 3428 Size: 393 559 31 141 177 165 459 264 Cuts at: 3428 4277 4874 5661 5929 1239 Size: 849 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 559 459 393 268 264 177 165 141 31 EcoNI CCTnn'n_nnAGG Cuts at: 2888 3026 3371 2888 Size: 138 345 5731 Fragments arranged by size: 5731 345 138 EcoO109I rG'GnC_Cy Cuts at: 2095 2231 2515 2095 Size: 136 284 5794 Fragments arranged by size: 5794 284 136 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 3136 2788 Size: 348 5866 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 2045 2419 3286 3367 5361 1678 Size: 367 374 867 81 1994 2531 Fragments arranged by size: 2531 1994 867 374 367 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 3418 3450 4855 Size: 32 135 32 863 32 951 32 1405 Cuts at: 4855 4887 4929 4961 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1405 951 863 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3804 669 Size: 988 281 1620 246 3079 Fragments arranged by size: 3079 1620 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KasI G'GCGC_C Cuts at: 3363 3363 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5328 5512 1305 Size: 4023 184 2007 Fragments arranged by size: 4023 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 2643 10 Size: 54 1425 343 666 145 3581 Fragments arranged by size: 3581 1425 666 343 145 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 2720 3449 4172 4531 4690 518 Size: 648 1216 338 729 723 359 159 2042 Fragments arranged by size: 2042 1216 729 723 648 359 338 159 MspA1I CmG'CkG Cuts at: 1606 3200 3630 4307 5455 5700 1606 Size: 1594 430 677 1148 245 2120 Fragments arranged by size: 2120 1594 1148 677 430 245 NarI GG'CG_CC Cuts at: 3364 3364 Size: 6214 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3346 3572 5117 1132 Size: 97 2117 226 1545 2229 Fragments arranged by size: 2229 2117 1545 226 97 PasI CC'CwG_GG Cuts at: 1348 2349 1348 Size: 1001 5213 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 2683 2760 2836 1108 Size: 1119 456 77 76 4486 Fragments arranged by size: 4486 1119 456 77 76 PfoI T'CCnGG_A Cuts at: 2220 2340 2220 Size: 120 6094 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PmlI CAC'GTG Cuts at: 3325 3403 3325 Size: 78 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2710 4283 4315 5796 5828 2678 Size: 32 1573 32 1481 32 3064 Fragments arranged by size: 3064 1573 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 2094 2231 2094 Size: 137 6077 PstI C_TGCA'G Cuts at: 830 2166 2333 2538 2944 3566 830 Size: 1336 167 205 406 622 3478 Fragments arranged by size: 3478 1336 622 406 205 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 3200 3200 Size: 6214 SacI G_AGCT'C Cuts at: 2883 3167 2883 Size: 284 5930 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 2333 3566 2333 Size: 1233 4981 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SfcI C'TryA_G Cuts at: 826 1045 2162 2329 2359 2534 2940 3457 3562 Size: 219 1117 167 30 175 406 517 105 Cuts at: 3562 4697 5378 5569 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 681 517 406 219 191 175 167 105 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2640 2640 Size: 6214 SfoI GGC'GCC Cuts at: 3365 3365 Size: 6214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 2546 3541 1872 Size: 674 995 4545 Fragments arranged by size: 4545 995 674 SmlI C'TyrA_G Cuts at: 820 1017 1943 3110 3435 4318 5219 5481 820 Size: 197 926 1167 325 883 901 262 1553 Fragments arranged by size: 1553 1167 926 901 883 325 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3346 3572 1132 Size: 2214 226 3774 Fragments arranged by size: 3774 2214 226 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 2780 3262 3475 1517 Size: 1263 482 213 4256 Fragments arranged by size: 4256 1263 482 213 StyI C'CwwG_G Cuts at: 513 1065 2004 2099 2457 2754 3653 513 Size: 552 939 95 358 297 899 3074 Fragments arranged by size: 3074 939 899 552 358 297 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 4241 4258 4411 4596 5041 325 Size: 1255 761 1900 17 153 185 445 1498 Fragments arranged by size: 1900 1498 1255 761 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4460 6172 96 Size: 274 80 33 51 1443 2483 1712 138 Fragments arranged by size: 2483 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2145 3197 3329 2145 Size: 1052 132 5030 Fragments arranged by size: 5030 1052 132 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 2544 3539 1870 Size: 674 995 4545 Fragments arranged by size: 4545 995 674 XmnI GAAnn'nnTTC Cuts at: 3413 4343 3413 Size: 930 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2566 4022 276 Size: 53 83 186 936 1032 1456 2468 Fragments arranged by size: 2468 1456 1032 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI