Restriction map for
pFN21AB7183
(Linear) (Six-base) MAPSORT of: KIBB7183.seq Check: 7999 from: 1 to: 1509
KIBB7183.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 197 1509
Size: 197 1312
AatII G_ACGT'C
Cuts at: 0 563 1509
Size: 563 946
AccI GT'mk_AC
Cuts at: 0 1451 1509
Size: 1451 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1159 1423 1509
Size: 535 624 264 86
Fragments arranged by size:
624 535 264 86
AfeI AGC'GCT
Cuts at: 0 1279 1509
Size: 1279 230
AleI CACnn'nnGTG
Cuts at: 0 377 1509
Size: 377 1132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 1509
Size: 42 1467
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 673 705 1509
Size: 673 32 804
Fragments arranged by size:
804 673 32
AlwNI CAG_nnn'CTG
Cuts at: 0 222 513 831 1017 1509
Size: 222 291 318 186 492
Fragments arranged by size:
492 318 291 222 186
ApaI G_GGCC'C
Cuts at: 0 93 230 1509
Size: 93 137 1279
Fragments arranged by size:
1279 137 93
AvaI C'yCGr_G
Cuts at: 0 86 539 1251 1351 1509
Size: 86 453 712 100 158
Fragments arranged by size:
712 453 158 100 86
AvrII C'CTAG_G
Cuts at: 0 94 1509
Size: 94 1415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 792 825 1509
Size: 792 33 684
Fragments arranged by size:
792 684 33
BanI G'GyrC_C
Cuts at: 0 1358 1509
Size: 1358 151
BanII G_rGCy'C
Cuts at: 0 93 230 344 878 1162 1300 1509
Size: 93 137 114 534 284 138 209
Fragments arranged by size:
534 284 209 138 137 114 93
BbeI G_GCGC'C
Cuts at: 0 1362 1509
Size: 1362 147
BbvCI CC'TCA_GC
Cuts at: 0 148 1509
Size: 148 1361
BciVI GTATCCnnnnn_n'
Cuts at: 0 820 1509
Size: 820 689
BglI GCCn_nnn'nGGC
Cuts at: 0 88 408 635 913 1509
Size: 88 320 227 278 596
Fragments arranged by size:
596 320 278 227 88
BglII A'GATC_T
Cuts at: 0 161 1509
Size: 161 1348
Bme1580I G_kGCm'C
Cuts at: 0 63 69 76 93 230 374 1325 1509
Size: 63 6 7 17 137 144 951 184
Fragments arranged by size:
951 184 144 137 63 17 7 6
BmgBI CAC'GTC
Cuts at: 0 1005 1478 1509
Size: 1005 473 31
Fragments arranged by size:
1005 473 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 641 1509
Size: 79 562 868
Fragments arranged by size:
868 562 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 749 1509
Size: 749 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 217 358 700 1509
Size: 186 31 141 342 809
Fragments arranged by size:
809 342 186 141 31
Bpu10I CC'TnA_GC
Cuts at: 0 148 1259 1509
Size: 148 1111 250
Fragments arranged by size:
1111 250 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1415 1509
Size: 1090 325 94
Fragments arranged by size:
1090 325 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 1105 1509
Size: 1105 404
BsaAI yAC'GTr
Cuts at: 0 1320 1398 1509
Size: 1320 78 111
Fragments arranged by size:
1320 111 78
BsaHI Gr'CG_yC
Cuts at: 0 560 1359 1509
Size: 560 799 150
Fragments arranged by size:
799 560 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 673 703 865 895 1509
Size: 673 30 162 30 614
Fragments arranged by size:
673 614 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1477 1509
Size: 1477 32
BseYI C'CCAG_C
Cuts at: 0 125 222 568 624 1191 1195 1227 1274
Size: 125 97 346 56 567 4 32 47
Cuts at: 1274 1509
Size: 235
Fragments arranged by size:
567 346 235 125 97 56 47 32
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 481 1027 1375 1509
Size: 175 306 546 348 134
Fragments arranged by size:
546 348 306 175 134
BsiHKAI G_wGCw'C
Cuts at: 0 188 767 878 1162 1509
Size: 188 579 111 284 347
Fragments arranged by size:
579 347 284 188 111
Bsp1286I G_dGCh'C
Cuts at: 0 63 69 76 93 188 230 344 374
Size: 63 6 7 17 95 42 114 30
Cuts at: 374 767 878 1162 1300 1325 1509
Size: 393 111 284 138 25 184
Fragments arranged by size:
393 284 184 138 114 111 95 63
42 30 25 17 7 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 1509
Size: 197 1312
BsrBI CCG'CTC
Cuts at: 0 85 621 1263 1509
Size: 85 536 642 246
Fragments arranged by size:
642 536 246 85
BsrDI GCAATG_nn'
Cuts at: 0 1242 1509
Size: 1242 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1017 1398 1509
Size: 1017 381 111
Fragments arranged by size:
1017 381 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 1509
Size: 924 585
BstYI r'GATC_y
Cuts at: 0 161 1013 1509
Size: 161 852 496
Fragments arranged by size:
852 496 161
BtsI GCAGTG_nn'
Cuts at: 0 494 932 1509
Size: 494 438 577
Fragments arranged by size:
577 494 438
DraIII CAC_nnn'GTG
Cuts at: 0 1320 1398 1509
Size: 1320 78 111
Fragments arranged by size:
1320 111 78
EaeI y'GGCC_r
Cuts at: 0 491 636 1034 1509
Size: 491 145 398 475
Fragments arranged by size:
491 475 398 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 886 904 1509
Size: 886 18 605
Fragments arranged by size:
886 605 18
EcoICRI GAG'CTC
Cuts at: 0 876 1160 1509
Size: 876 284 349
Fragments arranged by size:
876 349 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 217 358 535 700 1159 1423 1509
Size: 186 31 141 177 165 459 264 86
Fragments arranged by size:
459 264 186 177 165 141 86 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 883 1021 1366 1509
Size: 883 138 345 143
Fragments arranged by size:
883 345 143 138
EcoO109I rG'GnC_Cy
Cuts at: 0 90 226 510 1509
Size: 90 136 284 999
Fragments arranged by size:
999 284 136 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 1131 1509
Size: 783 348 378
Fragments arranged by size:
783 378 348
HaeII r_GCGC'y
Cuts at: 0 40 414 1281 1362 1509
Size: 40 374 867 81 147
Fragments arranged by size:
867 374 147 81 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 1413 1445 1509
Size: 430 32 951 32 64
Fragments arranged by size:
951 430 64 32 32
KasI G'GCGC_C
Cuts at: 0 1358 1509
Size: 1358 151
MscI TGG'CCA
Cuts at: 0 493 638 1509
Size: 493 145 871
Fragments arranged by size:
871 493 145
MslI CAynn'nnrTG
Cuts at: 0 377 715 1444 1509
Size: 377 338 729 65
Fragments arranged by size:
729 377 338 65
MspA1I CmG'CkG
Cuts at: 0 1195 1509
Size: 1195 314
NarI GG'CG_CC
Cuts at: 0 1359 1509
Size: 1359 150
NspI r_CATG'y
Cuts at: 0 1341 1509
Size: 1341 168
PasI CC'CwG_GG
Cuts at: 0 344 1509
Size: 344 1165
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 678 755 831 1509
Size: 222 456 77 76 678
Fragments arranged by size:
678 456 222 77 76
PfoI T'CCnGG_A
Cuts at: 0 215 335 1509
Size: 215 120 1174
Fragments arranged by size:
1174 215 120
PmlI CAC'GTG
Cuts at: 0 1320 1398 1509
Size: 1320 78 111
Fragments arranged by size:
1320 111 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1509
Size: 673 32 804
Fragments arranged by size:
804 673 32
PspOMI G'GGCC_C
Cuts at: 0 89 226 1509
Size: 89 137 1283
Fragments arranged by size:
1283 137 89
PstI C_TGCA'G
Cuts at: 0 161 328 533 939 1509
Size: 161 167 205 406 570
Fragments arranged by size:
570 406 205 167 161
PvuII CAG'CTG
Cuts at: 0 1195 1509
Size: 1195 314
SacI G_AGCT'C
Cuts at: 0 878 1162 1509
Size: 878 284 347
Fragments arranged by size:
878 347 284
SbfI CC_TGCA'GG
Cuts at: 0 328 1509
Size: 328 1181
SfcI C'TryA_G
Cuts at: 0 157 324 354 529 935 1452 1509
Size: 157 167 30 175 406 517 57
Fragments arranged by size:
517 406 175 167 157 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 635 1509
Size: 635 874
SfoI GGC'GCC
Cuts at: 0 1360 1509
Size: 1360 149
SmaI CCC'GGG
Cuts at: 0 541 1509
Size: 541 968
SmlI C'TyrA_G
Cuts at: 0 1105 1430 1509
Size: 1105 325 79
Fragments arranged by size:
1105 325 79
SphI G_CATG'C
Cuts at: 0 1341 1509
Size: 1341 168
StuI AGG'CCT
Cuts at: 0 775 1257 1470 1509
Size: 775 482 213 39
Fragments arranged by size:
775 482 213 39
StyI C'CwwG_G
Cuts at: 0 94 452 749 1509
Size: 94 358 297 760
Fragments arranged by size:
760 358 297 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1509
Size: 336 1173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 140 1192 1324 1509
Size: 140 1052 132 185
Fragments arranged by size:
1052 185 140 132
XmaI C'CCGG_G
Cuts at: 0 539 1509
Size: 539 970
XmnI GAAnn'nnTTC
Cuts at: 0 1408 1509
Size: 1408 101
ZraI GAC'GTC
Cuts at: 0 561 1509
Size: 561 948
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlfI AloI
AlwNI ApaI AvaI AvrII BaeI BanI BanII BbeI
BbvCI BciVI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstXI BstYI BtsI DraIII EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI
MspA1I NarI NspI PasI PflMI PfoI PmlI PpiI
PspOMI PstI PvuII SacI SbfI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI ApaLI ApoI
AscI AseI AsiSI BamHI BbsI BcgI BclI BfrBI
BlpI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EarI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI