Restriction map for
pFN21AE0215
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BseAI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI Ksp632I KspI MaeI MaeIII
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 230 GGTACC G^GTACC
AccI 322 GTCTAC GT^[AC][GT]AC
AlwI 135 GATCC GGATC(4/5)
Aor51HI 278 AGCGCT AGC^GCT
Asp718I 230 GGTACC G^GTACC
BanI 230 GGTACC G^G[CT][GA]CC
BcnI 119 CCCGG CC^[GC]GG
BsaBI 198 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 234 CCGAGG C^C[ACGT][ACGT]GG
BsaWI 27 TCCGGT [AT]^CCGG[AT]
BseRI 190 GAGGAG GAGGAG(10/8)
BsgI 253 CTGCAC GTGCAG(16/14)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BsiYI 234 CCGAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 234 CCGAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 80 TGTACA T^GTACA
BsrGI 80 TGTACA T^GTACA
BstAPI 246 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 134 AGATCC [GA]^GATC[CT]
DpnII 135 GATC ^GATC
DraII 126 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 50 CGGCCG [CT]^GGCC[GA]
EagI 50 CGGCCG C^GGCCG
EclXI 50 CGGCCG C^GGCCG
Eco47III 278 AGCGCT AGC^GCT
Eco52I 50 CGGCCG C^GGCCG
EcoO109I 126 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 180 CCCGC CCCGC(4/6)
HaeIII 51 GGCC GG^CC
HphI 209 TCACC GGTGA(8/7)
KpnI 230 GGTACC GGTAC^C
MaeII 149 ACGT A^CGT
MamI 198 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 135 GATC ^GATC
MflI 134 AGATCC [GA]^GATC[CT]
NciI 119 CCCGG CC^[GC]GG
NdeII 135 GATC ^GATC
NlaIII 36 CATG CATG^
NotI 49 GCGGCCGC GC^GGCCGC
PpuMI 126 GGGACCC [GA]G^G[AT]CC[CT]
PstI 265 CTGCAG CTGCA^G
Sau3AI 135 GATC ^GATC
SspBI 80 TGTACA T^GTACA
XhoII 134 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 158 AGCT AG^CT
263 AGCT
AvaII 101 GGTCC G^G[AT]CC
127 GGACC
BsmFI 126 GGGAC GGGAC(10/14)
3 GTCCC
Cac8I 72 GCTGGC GC[ACGT]^[ACGT]GC
251 GCCTGC
Cfr13I 101 GGTCC G^G[ACGT]CC
127 GGACC
HaeII 75 GGCGCT [GA]GCGC^[CT]
278 AGCGCT
HapII 28 CCGG C^CGG
120 CCGG
HpaII 28 CCGG C^CGG
120 CCGG
MspI 28 CCGG C^CGG
120 CCGG
Sau96I 101 GGTCC G^G[ACGT]CC
127 GGACC
SfcI 141 CTACAG C^T[GA][CT]AG
265 CTGCAG
TspRI 184 CACTG CA[GC]TG[ACGT][ACGT]^
247 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI AfaI AspHI BbvI
BmyI BsiHKAI Bsp1286I BssKI BstNI
BstUI CfoI EcoRII Fnu4HI HhaI
HinP1I ItaI MboII MnlI MvaI
MvnI MwoI NlaIV RsaI ScrFI
TseI