(Circular) (Six-base) MAPSORT of: pFN21AE0209.seq Check: 3786 from: 1 to: 5614 pFN21AE0209 5614 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3677 5061 5329 1632 Size: 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 268 AfeI AGC'GCT Cuts at: 2302 2302 Size: 5614 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 4513 5490 1203 Size: 3310 977 1327 Fragments arranged by size: 3310 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AlwNI CAG_nnn'CTG Cuts at: 3357 4929 3357 Size: 1572 4042 ApaI G_GGCC'C Cuts at: 2287 2287 Size: 5614 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2528 2785 2923 3149 3301 5410 1493 Size: 1035 257 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 257 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2939 1870 Size: 73 996 4545 Fragments arranged by size: 4545 996 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2858 2935 4390 618 Size: 325 217 627 1071 77 1455 1842 Fragments arranged by size: 1842 1455 1071 627 325 217 77 BanII G_rGCy'C Cuts at: 1391 2287 2933 1391 Size: 896 646 4072 Fragments arranged by size: 4072 896 646 BbeI G_GCGC'C Cuts at: 2862 2862 Size: 5614 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BbvCI CC'TCA_GC Cuts at: 2822 2822 Size: 5614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 4225 136 Size: 107 122 71 631 605 354 2199 1525 Fragments arranged by size: 2199 1525 631 605 354 122 107 71 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2287 3675 4831 1790 Size: 497 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 497 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4303 442 Size: 416 53 83 399 24 1928 958 1753 Fragments arranged by size: 1928 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2587 4274 1239 Size: 1348 1687 2579 Fragments arranged by size: 2579 1687 1348 Bpu10I CC'TnA_GC Cuts at: 2822 2822 Size: 5614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3739 4604 4902 3739 Size: 865 298 4451 Fragments arranged by size: 4451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 4277 882 Size: 617 954 1824 2219 Fragments arranged by size: 2219 1824 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 2321 5608 2321 Size: 3287 2327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2859 3421 3803 4438 Size: 53 83 186 936 1326 562 382 635 Cuts at: 4438 275 Size: 1451 Fragments arranged by size: 1451 1326 936 635 562 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2550 2681 2781 4047 4445 4719 4866 5277 2550 Size: 131 100 1266 398 274 147 411 2887 Fragments arranged by size: 2887 1266 411 398 274 147 131 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2378 2408 2570 Size: 30 165 30 855 30 90 30 162 Cuts at: 2570 2600 1178 Size: 30 4192 Fragments arranged by size: 4192 855 165 162 90 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2897 1186 Size: 1711 3903 BseYI C'CCAG_C Cuts at: 2137 2179 2199 2280 2741 4817 5313 2137 Size: 42 20 81 461 2076 496 2438 Fragments arranged by size: 2438 2076 496 461 81 42 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 1163 Size: 573 648 4393 Fragments arranged by size: 4393 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2033 3064 3825 4853 1540 Size: 275 186 32 1031 761 1028 2301 Fragments arranged by size: 2301 1031 1028 761 275 186 32 BsiHKAI G_wGCw'C Cuts at: 2889 3675 3760 4831 2889 Size: 786 85 1071 3672 Fragments arranged by size: 3672 1071 786 85 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2889 2933 3675 3760 4831 1391 Size: 399 497 602 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 497 399 85 44 BspEI T'CCGG_A Cuts at: 2550 2781 5277 2550 Size: 231 2496 2887 Fragments arranged by size: 2887 2496 231 BspHI T'CATG_A Cuts at: 2164 3503 5233 2164 Size: 1339 1730 2545 Fragments arranged by size: 2545 1730 1339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 2471 2969 844 Size: 1264 363 498 3489 Fragments arranged by size: 3489 1264 498 363 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2218 2530 4109 4283 65 Size: 1183 970 312 1579 174 1396 Fragments arranged by size: 1579 1396 1183 970 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 4258 4445 1700 Size: 110 296 2152 187 2869 Fragments arranged by size: 2869 2152 296 187 110 BsrGI T'GTAC_A Cuts at: 96 2891 96 Size: 2795 2819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 2045 3674 4686 2045 Size: 1629 1012 2973 Fragments arranged by size: 2973 1629 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5614 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2185 2944 3311 3696 3713 5165 5251 5263 Size: 213 759 367 385 17 1452 86 12 Cuts at: 5263 5609 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 759 385 367 346 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2801 513 Size: 552 939 797 3326 Fragments arranged by size: 3326 939 797 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 3061 3950 Size: 54 1425 50 275 18 200 1031 889 Cuts at: 3950 5579 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1031 889 275 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2030 3061 1537 Size: 275 218 1031 4090 Fragments arranged by size: 4090 1031 275 218 EarI CTCTTCn'nnn_ Cuts at: 1353 3544 1353 Size: 2191 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 2324 4201 4575 4721 387 Size: 1064 138 486 249 1877 374 146 1280 Fragments arranged by size: 1877 1280 1064 486 374 249 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2587 3677 4274 5061 5329 1239 Size: 393 955 1090 597 787 268 1524 Fragments arranged by size: 1524 1090 955 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2902 2902 Size: 5614 EcoRI G'AATT_C Cuts at: 2528 2785 2923 3301 2528 Size: 257 138 378 4841 Fragments arranged by size: 4841 378 257 138 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 2304 2862 4761 1678 Size: 626 558 1899 2531 Fragments arranged by size: 2531 1899 626 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 2570 2602 4255 Size: 32 135 32 587 32 379 32 1653 Cuts at: 4255 4287 4329 4361 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1653 587 379 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2958 3204 669 Size: 988 281 289 731 246 3079 Fragments arranged by size: 3079 988 731 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KasI G'GCGC_C Cuts at: 2858 2858 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3792 Fragments arranged by size: 3792 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3572 3931 4090 518 Size: 648 2406 359 159 2042 Fragments arranged by size: 2406 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2183 2643 3030 3707 4855 5100 1606 Size: 531 46 460 387 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 460 387 245 46 NarI GG'CG_CC Cuts at: 2859 2859 Size: 5614 NcoI C'CATG_G Cuts at: 513 1065 2004 2801 513 Size: 552 939 797 3326 Fragments arranged by size: 3326 939 797 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 2030 3061 2030 Size: 1031 4583 NspI r_CATG'y Cuts at: 1132 1229 2523 2972 4517 1132 Size: 97 1294 449 1545 2229 Fragments arranged by size: 2229 1545 1294 449 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 2199 1108 Size: 1091 4523 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2902 2902 Size: 5614 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PspOMI G'GGCC_C Cuts at: 2283 2283 Size: 5614 PstI C_TGCA'G Cuts at: 830 2966 830 Size: 2136 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 PvuII CAG'CTG Cuts at: 2643 2643 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SanDI GG'GwC_CC Cuts at: 2902 2902 Size: 5614 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 2771 3862 5574 2771 Size: 1091 1712 2811 Fragments arranged by size: 2811 1712 1091 SfcI C'TryA_G Cuts at: 826 1045 2962 4097 4778 4969 826 Size: 219 1917 1135 681 191 1471 Fragments arranged by size: 1917 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2860 2860 Size: 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3718 4619 4881 820 Size: 197 926 1775 901 262 1553 Fragments arranged by size: 1775 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 2801 3053 513 Size: 552 939 477 320 252 3074 Fragments arranged by size: 3074 939 552 477 320 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2733 3641 3658 3811 3996 4441 325 Size: 1255 1153 908 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1153 908 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 2891 3860 Size: 274 80 33 51 1443 792 122 969 Cuts at: 3860 5572 96 Size: 1712 138 Fragments arranged by size: 1712 1443 969 792 274 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 5574 5606 2477 Size: 32 3065 32 2485 Fragments arranged by size: 3065 2485 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI