(Circular) (Six-base) MAPSORT of: pFN21AB6329.seq Check: 3292 from: 1 to: 5488 pFN21AB6329 5488 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2638 2638 Size: 5488 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 2831 1937 Size: 448 446 4594 Fragments arranged by size: 4594 448 446 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3551 4935 5203 1632 Size: 1919 1384 268 1917 Fragments arranged by size: 1919 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 2388 2751 4387 5364 1203 Size: 1185 363 1636 977 1327 Fragments arranged by size: 1636 1327 1185 977 363 AgeI A'CCGG_T Cuts at: 2665 4319 2665 Size: 1654 3834 AhdI GACnn_n'nnGTC Cuts at: 2080 4217 2080 Size: 2137 3351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 2492 3231 4803 2492 Size: 739 1572 3177 Fragments arranged by size: 3177 1572 739 ApaI G_GGCC'C Cuts at: 2057 2527 2057 Size: 470 5018 ApaLI G'TGCA_C Cuts at: 2309 3545 4701 2309 Size: 1236 1156 3096 Fragments arranged by size: 3096 1236 1156 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2813 1870 Size: 73 106 764 4545 Fragments arranged by size: 4545 764 106 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 2207 2809 4264 618 Size: 325 217 627 395 25 602 1455 1842 Fragments arranged by size: 1842 1455 627 602 395 325 217 25 BanII G_rGCy'C Cuts at: 1391 2057 2439 2527 2706 2807 1391 Size: 666 382 88 179 101 4072 Fragments arranged by size: 4072 666 382 179 101 88 BbeI G_GCGC'C Cuts at: 2186 2211 2186 Size: 25 5463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2542 2635 928 Size: 971 643 93 3781 Fragments arranged by size: 3781 971 643 93 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2526 2560 3679 3713 1903 Size: 34 589 34 1119 34 3678 Fragments arranged by size: 3678 1119 589 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2522 4099 136 Size: 107 122 71 631 605 850 1577 1525 Fragments arranged by size: 1577 1525 850 631 605 122 107 71 BglII A'GATC_T Cuts at: 2274 5483 2274 Size: 3209 2279 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2057 2313 2527 3549 4705 1790 Size: 267 256 214 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 267 256 214 BmgBI CAC'GTC Cuts at: 1318 2752 1318 Size: 1434 4054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 2480 3219 Size: 416 53 83 399 24 805 258 739 Cuts at: 3219 4177 442 Size: 958 1753 Fragments arranged by size: 1753 958 805 739 416 399 258 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2466 1056 Size: 1410 4078 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4148 1239 Size: 2909 2579 Bpu10I CC'TnA_GC Cuts at: 2217 2579 2765 2217 Size: 362 186 4940 Fragments arranged by size: 4940 362 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3613 4478 4776 3613 Size: 865 298 4325 Fragments arranged by size: 4325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 2589 4151 882 Size: 617 795 295 1562 2219 Fragments arranged by size: 2219 1562 795 617 295 BsaAI yAC'GTr Cuts at: 493 1204 2389 2569 3279 493 Size: 711 1185 180 710 2702 Fragments arranged by size: 2702 1185 711 710 180 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 2208 3295 3677 Size: 53 83 186 936 650 25 1087 382 Cuts at: 3677 4312 275 Size: 635 1451 Fragments arranged by size: 1451 1087 936 650 635 382 186 83 53 25 BsaWI w'CCGG_w Cuts at: 2665 3921 4319 4593 4740 5151 2665 Size: 1256 398 274 147 411 3002 Fragments arranged by size: 3002 1256 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5263 Fragments arranged by size: 5263 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2213 2705 1186 Size: 871 156 492 3969 Fragments arranged by size: 3969 871 492 156 BseYI C'CCAG_C Cuts at: 4691 5187 4691 Size: 496 4992 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4915 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2938 3699 4727 1540 Size: 275 186 18 919 761 1028 2301 Fragments arranged by size: 2301 1028 919 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2313 2439 2613 2706 2766 3549 3634 4705 2313 Size: 126 174 93 60 783 85 1071 3096 Fragments arranged by size: 3096 1071 783 174 126 93 85 60 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2313 2439 2527 2613 2706 2766 Size: 399 267 256 126 88 86 93 60 Cuts at: 2766 2807 3549 3634 4705 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 256 126 93 88 86 85 60 41 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 2505 2607 2638 2843 844 Size: 1403 258 102 31 205 3489 Fragments arranged by size: 3489 1403 258 205 102 31 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2583 2665 4132 4319 1700 Size: 110 773 82 1467 187 2869 Fragments arranged by size: 2869 1467 773 187 110 82 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 2674 3548 4560 2674 Size: 874 1012 3602 Fragments arranged by size: 3602 1012 874 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 2072 1184 Size: 888 4600 BstYI r'GATC_y Cuts at: 1972 2274 2818 3185 3570 3587 5039 5125 5137 Size: 302 544 367 385 17 1452 86 12 Cuts at: 5137 5483 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 544 385 367 346 302 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2247 2247 Size: 5488 BtgI C'CryG_G Cuts at: 513 1065 2004 2774 513 Size: 552 939 770 3227 Fragments arranged by size: 3227 939 770 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 2457 3004 3798 3818 2457 Size: 547 794 20 4127 Fragments arranged by size: 4127 794 547 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2777 2935 Size: 54 1425 50 275 18 186 761 158 Cuts at: 2935 3824 5453 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 761 275 186 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 2935 1537 Size: 275 204 919 4090 Fragments arranged by size: 4090 919 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3418 1353 Size: 782 1283 3423 Fragments arranged by size: 3423 1283 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4075 4449 4595 387 Size: 1064 138 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2704 2437 Size: 267 5221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3551 4148 4935 5203 1239 Size: 393 1919 597 787 268 1524 Fragments arranged by size: 1919 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2053 2161 2514 2575 2053 Size: 108 353 61 4966 Fragments arranged by size: 4966 353 108 61 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 2186 2211 4635 1678 Size: 508 25 2424 2531 Fragments arranged by size: 2531 2424 508 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2741 2773 4129 Size: 32 135 32 494 32 643 32 1356 Cuts at: 4129 4161 4203 4235 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 643 494 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KasI G'GCGC_C Cuts at: 2182 2207 2182 Size: 25 5463 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3666 Fragments arranged by size: 3666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2409 3446 3805 3964 518 Size: 648 1188 55 1037 359 159 2042 Fragments arranged by size: 2042 1188 1037 648 359 159 55 MspA1I CmG'CkG Cuts at: 1606 2059 2178 2904 3581 4729 4974 1606 Size: 453 119 726 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 453 245 119 NarI GG'CG_CC Cuts at: 2183 2208 2183 Size: 25 5463 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3997 Fragments arranged by size: 3997 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NheI G'CTAG_C Cuts at: 1052 2462 1052 Size: 1410 4078 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PfoI T'CCnGG_A Cuts at: 2616 2616 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 2575 Size: 5488 PshAI GACnn'nnGTC Cuts at: 1613 2228 1613 Size: 615 4873 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PspOMI G'GGCC_C Cuts at: 2053 2523 2053 Size: 470 5018 PstI C_TGCA'G Cuts at: 830 2424 2840 830 Size: 1594 416 3478 Fragments arranged by size: 3478 1594 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SacI G_AGCT'C Cuts at: 2439 2706 2439 Size: 267 5221 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SexAI A'CCwGG_T Cuts at: 2379 2379 Size: 5488 SfcI C'TryA_G Cuts at: 826 1045 2420 2836 3971 4652 4843 826 Size: 219 1375 416 1135 681 191 1471 Fragments arranged by size: 1471 1375 1135 681 416 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2522 2522 Size: 5488 SfoI GGC'GCC Cuts at: 2184 2209 2184 Size: 25 5463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3592 4493 4755 820 Size: 197 926 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5488 StyI C'CwwG_G Cuts at: 513 1065 2004 2253 2927 513 Size: 552 939 249 674 3074 Fragments arranged by size: 3074 939 674 552 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3515 3532 3685 3870 4315 325 Size: 1255 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3734 5446 Size: 274 80 33 51 1443 777 980 1712 Cuts at: 5446 96 Size: 138 Fragments arranged by size: 1712 1443 980 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5448 5480 5448 Size: 32 5456 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SrfI SwaI XcmI