Restriction map for
pFN21AE0237
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI HincII HindII HindIII HpaI
HphI KpnI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NdeII NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI SbfI SexAI SfcI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AfaI 465 GTAC GT^AC
AluI 227 AGCT AG^CT
AspHI 333 GAGCAC G[AT]GC[AT]^C
AviII 322 TGCGCA TGC^GCA
BpmI 88 CTGGAG CTGGAG(16/14)
BseRI 151 GAGGAG GAGGAG(10/8)
BsiHKAI 333 GAGCAC G[AT]GC[AT]^C
BsmBI 205 GAGACG CGTCTC(1/5)
BsrDI 359 GCAATG GCAATG(2/0)
BstXI 173 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 34 CTGAG C^T[ACGT]AG
EarI 433 GAAGAG CTCTTC(1/4)
FspI 322 TGCGCA TGC^GCA
HgaI 207 GACGC GACGC(5/10)
Ksp632I 433 GAAGAG CTCTTC(1/4)
MboII 433 GAAGA GAAGA(8/7)
RsaI 465 GTAC GT^AC
ScaI 464 AGTACT AGT^ACT
SfaNI 452 GCATC GCATC(5/9)
SgrAI 103 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 455 TCGA T^CGA
TthHB8I 455 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 156 GGCGCC G[GA]^CG[CT]C
183 GGCGCC
ApaI 70 GGGCCC GGGCC^C
252 GGGCCC
AvaII 98 GGACC G^G[AT]CC
132 GGTCC
BbeI 156 GGCGCC GGCGC^C
183 GGCGCC
BglI 235 GCCGAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
396 GCCCGAGAGGC
BsaHI 156 GGCGCC G[GA]^CG[CT]C
183 GGCGCC
BsmAI 205 GAGAC GTCTC(1/5)
364 GTCTC
EagI 125 CGGCCG C^GGCCG
162 CGGCCG
EclXI 125 CGGCCG C^GGCCG
162 CGGCCG
Eco52I 125 CGGCCG C^GGCCG
162 CGGCCG
HaeII 156 GGCGCC [GA]GCGC^[CT]
183 GGCGCC
Hin1I 156 GGCGCC G[GA]^CG[CT]C
183 GGCGCC
HinfI 30 GACTC G^A[ACGT]TC
238 GAGTC
KasI 156 GGCGCC G^GCGCC
183 GGCGCC
NaeI 104 GCCGGC GCC^GGC
405 GCCGGC
NarI 156 GGCGCC GG^CGCC
183 GGCGCC
NgoMIV 104 GCCGGC G^CCGGC
405 GCCGGC
PleI 30 GACTC GAGTC(4/5)
238 GAGTC
PpuMI 97 GGGACCC [GA]G^G[AT]CC[CT]
131 AGGTCCC
SfoI 156 GGCGCC GGC^GCC
183 GGCGCC
SmaI 135 CCCGGG CCC^GGG
348 CCCGGG
XmaCI 135 CCCGGG C^CCGGG
348 CCCGGG
XmaI 135 CCCGGG C^CCGGG
348 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AvaI BanI BanII
BbvI BcnI BmyI BsaJI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI KspI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV SacII Sau96I ScrFI TseI