Restriction map for
pFN21AB8644
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# NO CUT ENZYMES ( 162 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstF5I BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI MvnI NaeI
NarI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 15 GACGTC GACGT^C
AccI 12 GTCGAC GT^[AC][GT]AC
AflIII 288 ACATGT A^C[GA][CT]GT
AlwNI 96 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 397 GTGCTC G[AT]GC[AT]^C
AspI 432 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 441 TGGCCA TGG^CCA
BanII 453 GGGCTC G[GA]GC[CT]^C
BbvCI 188 CCTCAGC CCTCAGC(-5/-2)
BbvI 92 GCAGC GCAGC(8/12)
BsaAI 313 TACGTG [CT]AC^GT[GA]
BsiHKAI 397 GTGCTC G[AT]GC[AT]^C
BsmAI 430 GAGAC GTCTC(1/5)
BsmBI 430 GAGACG CGTCTC(1/5)
BspLU11I 288 ACATGT A^CATGT
BssSI 427 CACGAG CACGAG(-5/-1)
BstEII 336 GGTGACC G^GT[ACGT]ACC
BstPI 336 GGTGACC G^GT[ACGT]ACC
DrdI 3 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 441 TGGCCA [CT]^GGCC[GA]
EcoNI 77 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 336 GGTGACC G^GT[ACGT]ACC
EcoT14I 243 CCATGG C^C[AT][AT]GG
Fnu4HI 92 GCAGC GC^[ACGT]GC
HapII 183 CCGG C^CGG
HgaI 212 GCGTC GACGC(5/10)
HincII 12 GTCGAC GT[CT]^[GA]AC
HindII 12 GTCGAC GT[CT]^[GA]AC
HinfI 10 GAGTC G^A[ACGT]TC
HpaII 183 CCGG C^CGG
ItaI 92 GCAGC GC^[ACGT]GC
MboII 281 TCTTC GAAGA(8/7)
MluNI 441 TGGCCA TGG^CCA
MscI 441 TGGCCA TGG^CCA
MspI 183 CCGG C^CGG
NcoI 243 CCATGG C^CATGG
NspI 288 ACATGT [GA]CATG^[CT]
PflFI 432 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 10 GAGTC GAGTC(4/5)
SalI 12 GTCGAC G^TCGAC
SmaI 182 CCCGGG CCC^GGG
StyI 243 CCATGG C^C[AT][AT]GG
TseI 92 GCAGC G^C[AT]GC
TspRI 320 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 432 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 182 CCCGGG C^CCGGG
XmaI 182 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 15 GACGTC G[GA]^CG[CT]C
211 GGCGTC
AfaI 286 GTAC GT^AC
312 GTAC
AluI 192 AGCT AG^CT
407 AGCT
BcnI 182 CCCGG CC^[GC]GG
183 CCGGG
BsaHI 15 GACGTC G[GA]^CG[CT]C
211 GGCGTC
BsrBI 37 CCGCTC CCGCTC(-3/-3)
106 GAGCGG
Cac8I 193 GCTGGC GC[ACGT]^[ACGT]GC
408 GCTGGC
DdeI 189 CTCAG C^T[ACGT]AG
404 CTCAG
DraII 128 GGGACCT [GA]G^G[ACGT]CC[CT]
163 AGGACCT
DsaI 243 CCATGG C^C[GA][CT]GG
386 CCGTGG
EcoO109I 128 GGGACCT [GA]G^G[ACGT]CC[CT]
163 AGGACCT
FauI 36 CCCGC CCCGC(4/6)
108 GCGGG
Hin1I 15 GACGTC G[GA]^CG[CT]C
211 GGCGTC
MaeIII 18 GTCAC ^GT[ACGT]AC
337 GTGAC
MspA1I 191 CAGCTG C[AC]G^C[GT]G
406 CAGCTG
NciI 182 CCCGG CC^[GC]GG
183 CCGGG
NlaIII 244 CATG CATG^
289 CATG
PflMI 381 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
444 CCAGGTCGTGG
PpuMI 128 GGGACCT [GA]G^G[AT]CC[CT]
163 AGGACCT
PvuII 191 CAGCTG CAG^CTG
406 CAGCTG
RsaI 286 GTAC GT^AC
312 GTAC
TaqI 13 TCGA T^CGA
302 TCGA
Tsp45I 18 GTCAC ^GT[GC]AC
337 GTGAC
TthHB8I 13 TCGA T^CGA
302 TCGA
Van91I 381 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
444 CCAGGTCGTGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AvaI AvaII BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI Cfr13I EcoRII
HaeIII HphI MaeII MnlI MvaI
MwoI NlaIV Sau96I ScrFI