(Circular) (Six-base) MAPSORT of: pFN21AB8644.seq Check: 5814 from: 1 to: 5164 pFN21AB8644 5164 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2024 2974 278 Size: 53 83 186 936 488 950 2468 Fragments arranged by size: 2468 950 936 488 186 83 53 AccI GT'mk_AC Cuts at: 1937 2018 2507 1937 Size: 81 489 4594 Fragments arranged by size: 4594 489 81 Acc65I G'GTAC_C Cuts at: 2485 2485 Size: 5164 AclI AA'CG_TT Cuts at: 1563 3291 3664 1563 Size: 1728 373 3063 Fragments arranged by size: 3063 1728 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 3227 4611 4879 1632 Size: 363 1232 1384 268 1917 Fragments arranged by size: 1917 1384 1232 363 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4967 AflIII A'CryG_T Cuts at: 1203 2293 4063 5040 1203 Size: 1090 1770 977 1327 Fragments arranged by size: 1770 1327 1090 977 AgeI A'CCGG_T Cuts at: 3995 3995 Size: 5164 AhdI GACnn_n'nnGTC Cuts at: 3893 3893 Size: 5164 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5164 AlwNI CAG_nnn'CTG Cuts at: 2106 2907 4479 2106 Size: 801 1572 2791 Fragments arranged by size: 2791 1572 801 ApaLI G'TGCA_C Cuts at: 3221 4377 3221 Size: 1156 4008 ApoI r'AATT_y Cuts at: 1493 2473 2699 2851 4960 1493 Size: 980 226 152 2109 1697 Fragments arranged by size: 2109 1697 980 226 152 AseI AT'TA_AT Cuts at: 160 1032 3718 160 Size: 872 2686 1606 Fragments arranged by size: 2686 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5164 AvaI C'yCGr_G Cuts at: 1870 1943 2130 2187 2264 2489 1870 Size: 73 187 57 77 225 4545 Fragments arranged by size: 4545 225 187 77 73 57 BamHI G'GATC_C Cuts at: 2494 2861 2494 Size: 367 4797 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2485 3940 618 Size: 325 217 627 698 1455 1842 Fragments arranged by size: 1842 1455 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 2462 2483 1391 Size: 1071 21 4072 Fragments arranged by size: 4072 1071 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4193 BbvCI CC'TCA_GC Cuts at: 2194 2194 Size: 5164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3355 3389 1903 Size: 34 1418 34 3678 Fragments arranged by size: 3678 1418 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3056 4266 3056 Size: 1210 3954 BclI T'GATC_A Cuts at: 1548 1548 Size: 5164 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3775 136 Size: 107 122 71 631 605 2103 1525 Fragments arranged by size: 2103 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5159 5159 Size: 5164 BlpI GC'TnA_GC Cuts at: 2581 2581 Size: 5164 Bme1580I G_kGCm'C Cuts at: 1790 2040 2360 3225 4381 1790 Size: 250 320 865 1156 2573 Fragments arranged by size: 2573 1156 865 320 250 BmgBI CAC'GTC Cuts at: 1318 2439 1318 Size: 1121 4043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 2057 2895 Size: 416 53 83 399 24 607 33 838 Cuts at: 2895 3853 442 Size: 958 1753 Fragments arranged by size: 1753 958 838 607 416 399 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3824 1239 Size: 2585 2579 Bpu10I CC'TnA_GC Cuts at: 2194 2194 Size: 5164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3289 4154 4452 3289 Size: 865 298 4001 Fragments arranged by size: 4001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3827 882 Size: 617 2328 2219 Fragments arranged by size: 2328 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2320 2955 493 Size: 711 1116 635 2702 Fragments arranged by size: 2702 1116 711 635 BsaBI GATnn'nnATC Cuts at: 5158 5158 Size: 5164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2217 2971 3353 Size: 53 83 186 936 488 196 754 382 Cuts at: 3353 3988 275 Size: 635 1451 Fragments arranged by size: 1451 936 754 635 488 382 196 186 83 53 BsaWI w'CCGG_w Cuts at: 3597 3995 4269 4416 4827 3597 Size: 398 274 147 411 3934 Fragments arranged by size: 3934 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4939 Fragments arranged by size: 4939 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5164 BseYI C'CCAG_C Cuts at: 2141 4367 4863 2141 Size: 2226 496 2442 Fragments arranged by size: 2442 2226 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4591 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2614 3375 4403 1540 Size: 275 186 613 761 1028 2301 Fragments arranged by size: 2301 1028 761 613 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 3225 3310 4381 2406 Size: 819 85 1071 3189 Fragments arranged by size: 3189 1071 819 85 BsmI GAATG_Cn' Cuts at: 2675 2768 2675 Size: 93 5071 BsmBI CGTCTCn'nnnn_ Cuts at: 2429 2429 Size: 5164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2360 2406 2462 2483 3225 3310 Size: 399 250 320 46 56 21 742 85 Cuts at: 3310 4381 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 320 250 85 56 46 21 BspEI T'CCGG_A Cuts at: 4827 4827 Size: 5164 BspHI T'CATG_A Cuts at: 3053 4783 3053 Size: 1730 3434 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2519 844 Size: 1675 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2113 3051 1394 Size: 429 221 69 938 3507 Fragments arranged by size: 3507 938 429 221 69 BsrDI GCAATG_nn' Cuts at: 65 1248 3659 3833 65 Size: 1183 2411 174 1396 Fragments arranged by size: 2411 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3808 3995 1700 Size: 110 1998 187 2869 Fragments arranged by size: 2869 1998 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5164 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5164 BssSI C'ACGA_G Cuts at: 2432 3224 4236 2432 Size: 792 1012 3360 Fragments arranged by size: 3360 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5164 BstBI TT'CG_AA Cuts at: 3968 3968 Size: 5164 BstEII G'GTnAC_C Cuts at: 1184 2341 1184 Size: 1157 4007 BstYI r'GATC_y Cuts at: 1972 2494 2861 3246 3263 4715 4801 4813 5159 Size: 522 367 385 17 1452 86 12 346 Cuts at: 5159 1972 Size: 1977 Fragments arranged by size: 1977 1452 522 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2248 2391 513 Size: 552 1183 143 3286 Fragments arranged by size: 3286 1183 552 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4425 Fragments arranged by size: 4425 683 56 BtsI GCAGTG_nn' Cuts at: 2680 3474 3494 2680 Size: 794 20 4350 Fragments arranged by size: 4350 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5164 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5129 DraI TTT'AAA Cuts at: 2468 2468 Size: 5164 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 4171 809 Size: 507 698 2157 1802 Fragments arranged by size: 2157 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2446 2611 3500 Size: 54 1425 50 275 18 616 165 889 Cuts at: 3500 5129 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 616 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2611 1537 Size: 275 799 4090 Fragments arranged by size: 4090 799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3094 1353 Size: 1741 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3751 4125 4271 387 Size: 1064 138 2162 374 146 1280 Fragments arranged by size: 2162 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 3227 3824 4611 4879 1239 Size: 393 363 1232 597 787 268 1524 Fragments arranged by size: 1524 1232 787 597 393 363 268 EcoNI CCTnn'n_nnAGG Cuts at: 2086 2086 Size: 5164 EcoO109I rG'GnC_Cy Cuts at: 2134 2169 2134 Size: 35 5129 EcoRI G'AATT_C Cuts at: 2473 2851 2473 Size: 378 4786 FspI TGC'GCA Cuts at: 3670 3670 Size: 5164 HaeII r_GCGC'y Cuts at: 1678 4311 1678 Size: 2633 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2258 2290 2426 Size: 32 135 32 434 32 220 32 136 Cuts at: 2426 2458 3805 3837 3879 3911 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 434 220 136 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2019 2508 2754 669 Size: 988 281 81 489 246 3079 Fragments arranged by size: 3079 988 489 281 246 81 HindIII A'AGCT_T Cuts at: 748 748 Size: 5164 HpaI GTT'AAC Cuts at: 2754 2754 Size: 5164 KpnI G_GTAC'C Cuts at: 2489 2489 Size: 5164 MfeI C'AATT_G Cuts at: 2763 2763 Size: 5164 MluI A'CGCG_T Cuts at: 5040 5040 Size: 5164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4278 4462 1305 Size: 2973 184 2007 Fragments arranged by size: 2973 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2448 10 Size: 54 1425 343 616 2726 Fragments arranged by size: 2726 1425 616 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3122 3481 3640 518 Size: 648 1956 359 159 2042 Fragments arranged by size: 2042 1956 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 2413 2580 3257 4405 4650 1606 Size: 592 215 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 592 245 215 167 NcoI C'CATG_G Cuts at: 513 1065 2248 513 Size: 552 1183 3429 Fragments arranged by size: 3429 1183 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5164 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5164 NotI GC'GGCC_GC Cuts at: 2611 2611 Size: 5164 NspI r_CATG'y Cuts at: 1132 1229 2297 2522 4067 1132 Size: 97 1068 225 1545 2229 Fragments arranged by size: 2229 1545 1068 225 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5164 PciI A'CATG_T Cuts at: 2293 4063 2293 Size: 1770 3394 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 2455 1108 Size: 1284 63 3817 Fragments arranged by size: 3817 1284 63 PmeI GTTT'AAAC Cuts at: 2468 2468 Size: 5164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3233 3265 4746 4778 3233 Size: 32 1481 32 3619 Fragments arranged by size: 3619 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2169 2134 Size: 35 5129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5164 PsiI TTA'TAA Cuts at: 2734 2734 Size: 5164 PstI C_TGCA'G Cuts at: 830 2516 830 Size: 1686 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5164 PvuII CAG'CTG Cuts at: 2198 2413 2198 Size: 215 4949 SalI G'TCGA_C Cuts at: 1936 2017 2506 1936 Size: 81 489 4594 Fragments arranged by size: 4594 489 81 SbfI CC_TGCA'GG Cuts at: 2516 2516 Size: 5164 ScaI AGT'ACT Cuts at: 3412 5124 3412 Size: 1712 3452 SfcI C'TryA_G Cuts at: 826 1045 2512 3647 4328 4519 826 Size: 219 1467 1135 681 191 1471 Fragments arranged by size: 1471 1467 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5164 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5164 SmaI CCC'GGG Cuts at: 1872 2189 2491 1872 Size: 317 302 4545 Fragments arranged by size: 4545 317 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 3268 4169 4431 820 Size: 197 926 1325 901 262 1553 Fragments arranged by size: 1553 1325 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2955 493 Size: 2462 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5164 SphI G_CATG'C Cuts at: 1132 2522 1132 Size: 1390 3774 SrfI GCCC'GGGC Cuts at: 2189 2189 Size: 5164 SspI AAT'ATT Cuts at: 5 52 3088 5 Size: 47 3036 2081 Fragments arranged by size: 3036 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5164 StyI C'CwwG_G Cuts at: 513 1065 2248 2603 513 Size: 552 1183 355 3074 Fragments arranged by size: 3074 1183 552 355 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3191 3208 3361 3546 3991 325 Size: 1255 1611 17 153 185 445 1498 Fragments arranged by size: 1611 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3410 5122 96 Size: 274 80 33 51 1443 1433 1712 138 Fragments arranged by size: 1712 1443 1433 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5124 5156 5124 Size: 32 5132 Tth111I GACn'n_nGTC Cuts at: 1316 2440 1316 Size: 1124 4040 XbaI T'CTAG_A Cuts at: 2500 2500 Size: 5164 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5164 XmaI C'CCGG_G Cuts at: 1870 2187 2489 1870 Size: 317 302 4545 Fragments arranged by size: 4545 317 302 XmnI GAAnn'nnTTC Cuts at: 3293 3293 Size: 5164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2022 2972 276 Size: 53 83 186 936 488 950 2468 Fragments arranged by size: 2468 950 936 488 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI