Restriction map for
pFN21AB8644
(Linear) (Six-base) MAPSORT of: KIBB8644.seq Check: 6356 from: 1 to: 459
KIBB8644.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 19 459
Size: 19 440
AccI GT'mk_AC
Cuts at: 0 13 459
Size: 13 446
AflIII A'CryG_T
Cuts at: 0 288 459
Size: 288 171
AlwNI CAG_nnn'CTG
Cuts at: 0 101 459
Size: 101 358
AvaI C'yCGr_G
Cuts at: 0 125 182 259 459
Size: 125 57 77 200
Fragments arranged by size:
200 125 77 57
BanII G_rGCy'C
Cuts at: 0 457 459
Size: 457 2
BbvCI CC'TCA_GC
Cuts at: 0 189 459
Size: 189 270
Bme1580I G_kGCm'C
Cuts at: 0 35 355 459
Size: 35 320 104
Fragments arranged by size:
320 104 35
BmgBI CAC'GTC
Cuts at: 0 434 459
Size: 434 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 52 459
Size: 19 33 407
Fragments arranged by size:
407 33 19
Bpu10I CC'TnA_GC
Cuts at: 0 189 459
Size: 189 270
BsaAI yAC'GTr
Cuts at: 0 315 459
Size: 315 144
BsaHI Gr'CG_yC
Cuts at: 0 16 212 459
Size: 16 196 247
Fragments arranged by size:
247 196 16
BseYI C'CCAG_C
Cuts at: 0 136 459
Size: 136 323
BsiHKAI G_wGCw'C
Cuts at: 0 401 459
Size: 401 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 424 459
Size: 424 35
Bsp1286I G_dGCh'C
Cuts at: 0 35 355 401 457 459
Size: 35 320 46 56 2
Fragments arranged by size:
320 56 46 35 2
BsrBI CCG'CTC
Cuts at: 0 39 108 459
Size: 39 69 351
Fragments arranged by size:
351 69 39
BssSI C'ACGA_G
Cuts at: 0 427 459
Size: 427 32
BstEII G'GTnAC_C
Cuts at: 0 336 459
Size: 336 123
BtgI C'CryG_G
Cuts at: 0 243 386 459
Size: 243 143 73
Fragments arranged by size:
243 143 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 459
Size: 9 450
EaeI y'GGCC_r
Cuts at: 0 441 459
Size: 441 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 81 459
Size: 81 378
EcoO109I rG'GnC_Cy
Cuts at: 0 129 164 459
Size: 129 35 295
Fragments arranged by size:
295 129 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 253 285 421 453 459
Size: 1 32 220 32 136 32 6
Fragments arranged by size:
220 136 32 32 32 6 1
HincII GTy'rAC
Cuts at: 0 14 459
Size: 14 445
MscI TGG'CCA
Cuts at: 0 443 459
Size: 443 16
MspA1I CmG'CkG
Cuts at: 0 193 408 459
Size: 193 215 51
Fragments arranged by size:
215 193 51
NcoI C'CATG_G
Cuts at: 0 243 459
Size: 243 216
NspI r_CATG'y
Cuts at: 0 292 459
Size: 292 167
PciI A'CATG_T
Cuts at: 0 288 459
Size: 288 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 450 459
Size: 387 63 9
Fragments arranged by size:
387 63 9
PpuMI rG'GwC_Cy
Cuts at: 0 129 164 459
Size: 129 35 295
Fragments arranged by size:
295 129 35
PvuII CAG'CTG
Cuts at: 0 193 408 459
Size: 193 215 51
Fragments arranged by size:
215 193 51
SalI G'TCGA_C
Cuts at: 0 12 459
Size: 12 447
SmaI CCC'GGG
Cuts at: 0 184 459
Size: 184 275
SrfI GCCC'GGGC
Cuts at: 0 184 459
Size: 184 275
StyI C'CwwG_G
Cuts at: 0 243 459
Size: 243 216
Tth111I GACn'n_nGTC
Cuts at: 0 435 459
Size: 435 24
XmaI C'CCGG_G
Cuts at: 0 182 459
Size: 182 277
ZraI GAC'GTC
Cuts at: 0 17 459
Size: 17 442
Enzymes that do cut:
AatII AccI AflIII AlwNI AvaI BanII BbvCI Bme1580I
BmgBI BmrI Bpu10I BsaAI BsaHI BseYI BsiHKAI BsmBI
Bsp1286I BsrBI BssSI BstEII BtgI DrdI EaeI EcoNI
EcoO109I Hin4I HincII MscI MspA1I NcoI NspI PciI
PflMI PpuMI PvuII SalI SmaI SrfI StyI Tth111I
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsgI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI TaqII TatI TstI XbaI XcmI
XhoI XmnI