(Linear) (Six-base) MAPSORT of: KIBB7626.seq Check: 4213 from: 1 to: 1116 KIBB7626.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1051 1116 Size: 1051 65 Acc65I G'GTAC_C Cuts at: 0 869 1116 Size: 869 247 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 944 1116 Size: 814 130 172 Fragments arranged by size: 814 172 130 AgeI A'CCGG_T Cuts at: 0 837 1116 Size: 837 279 AlwNI CAG_nnn'CTG Cuts at: 0 460 1116 Size: 460 656 ApaI G_GGCC'C Cuts at: 0 657 1116 Size: 657 459 BanI G'GyrC_C Cuts at: 0 138 196 521 551 869 1116 Size: 138 58 325 30 318 247 Fragments arranged by size: 325 318 247 138 58 30 BanII G_rGCy'C Cuts at: 0 103 657 1116 Size: 103 554 459 Fragments arranged by size: 554 459 103 BbeI G_GCGC'C Cuts at: 0 142 525 555 1116 Size: 142 383 30 561 Fragments arranged by size: 561 383 142 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 245 1116 Size: 245 871 BglI GCCn_nnn'nGGC Cuts at: 0 73 107 1116 Size: 73 34 1009 Fragments arranged by size: 1009 73 34 Bme1580I G_kGCm'C Cuts at: 0 199 657 1116 Size: 199 458 459 Fragments arranged by size: 459 458 199 BmgBI CAC'GTC Cuts at: 0 153 535 1116 Size: 153 382 581 Fragments arranged by size: 581 382 153 BmrI ACTGGGnnnn_n' Cuts at: 0 672 1116 Size: 672 444 BsaAI yAC'GTr Cuts at: 0 723 1116 Size: 723 393 BsaHI Gr'CG_yC Cuts at: 0 139 522 552 990 1008 1116 Size: 139 383 30 438 18 108 Fragments arranged by size: 438 383 139 108 30 18 BsaWI w'CCGG_w Cuts at: 0 837 1116 Size: 837 279 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 404 434 476 506 1116 Size: 404 30 42 30 610 Fragments arranged by size: 610 404 42 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 598 601 604 878 1116 Size: 598 3 3 274 238 Fragments arranged by size: 598 274 238 3 3 BseYI C'CCAG_C Cuts at: 0 577 906 1097 1116 Size: 577 329 191 19 Fragments arranged by size: 577 329 191 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 473 1116 Size: 473 643 BsiEI CG_ry'CG Cuts at: 0 68 206 1116 Size: 68 138 910 Fragments arranged by size: 910 138 68 BsiHKAI G_wGCw'C Cuts at: 0 825 1116 Size: 825 291 Bsp1286I G_dGCh'C Cuts at: 0 103 199 657 825 1116 Size: 103 96 458 168 291 Fragments arranged by size: 458 291 168 103 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 909 1051 1116 Size: 909 142 65 Fragments arranged by size: 909 142 65 BsrBI CCG'CTC Cuts at: 0 602 1116 Size: 602 514 BsrFI r'CCGG_y Cuts at: 0 229 695 837 1004 1116 Size: 229 466 142 167 112 Fragments arranged by size: 466 229 167 142 112 BsrGI T'GTAC_A Cuts at: 0 351 1116 Size: 351 765 BstAPI GCAn_nnn'nTGC Cuts at: 0 460 1116 Size: 460 656 BtgI C'CryG_G Cuts at: 0 71 128 191 200 382 555 978 1116 Size: 71 57 63 9 182 173 423 138 Fragments arranged by size: 423 182 173 138 71 63 57 9 BtsI GCAGTG_nn' Cuts at: 0 405 615 1113 1116 Size: 405 210 498 3 Fragments arranged by size: 498 405 210 3 EaeI y'GGCC_r Cuts at: 0 65 203 1116 Size: 65 138 913 Fragments arranged by size: 913 138 65 EagI C'GGCC_G Cuts at: 0 65 203 1116 Size: 65 138 913 Fragments arranged by size: 913 138 65 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 944 1116 Size: 814 130 172 Fragments arranged by size: 814 172 130 EcoNI CCTnn'n_nnAGG Cuts at: 0 46 494 1116 Size: 46 448 622 Fragments arranged by size: 622 448 46 EcoO109I rG'GnC_Cy Cuts at: 0 84 653 883 1116 Size: 84 569 230 233 Fragments arranged by size: 569 233 230 84 EcoRV GAT'ATC Cuts at: 0 985 1116 Size: 985 131 FspI TGC'GCA Cuts at: 0 397 1116 Size: 397 719 HaeII r_GCGC'y Cuts at: 0 142 525 555 1116 Size: 142 383 30 561 Fragments arranged by size: 561 383 142 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 1116 Size: 730 32 354 Fragments arranged by size: 730 354 32 KasI G'GCGC_C Cuts at: 0 138 521 551 1116 Size: 138 383 30 565 Fragments arranged by size: 565 383 138 30 KpnI G_GTAC'C Cuts at: 0 873 1116 Size: 873 243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 79 877 894 1116 Size: 79 798 17 222 Fragments arranged by size: 798 222 79 17 MspA1I CmG'CkG Cuts at: 0 73 113 130 202 666 702 1116 Size: 73 40 17 72 464 36 414 Fragments arranged by size: 464 414 73 72 40 36 17 NaeI GCC'GGC Cuts at: 0 231 1116 Size: 231 885 NarI GG'CG_CC Cuts at: 0 139 522 552 1116 Size: 139 383 30 564 Fragments arranged by size: 564 383 139 30 NgoMIV G'CCGG_C Cuts at: 0 229 1116 Size: 229 887 NotI GC'GGCC_GC Cuts at: 0 65 203 1116 Size: 65 138 913 Fragments arranged by size: 913 138 65 PflMI CCAn_nnn'nTGG Cuts at: 0 913 1116 Size: 913 203 PmlI CAC'GTG Cuts at: 0 723 1116 Size: 723 393 PpuMI rG'GwC_Cy Cuts at: 0 84 883 1116 Size: 84 799 233 Fragments arranged by size: 799 233 84 PsiI TTA'TAA Cuts at: 0 319 1116 Size: 319 797 PspOMI G'GGCC_C Cuts at: 0 653 1116 Size: 653 463 PstI C_TGCA'G Cuts at: 0 516 1116 Size: 516 600 PvuII CAG'CTG Cuts at: 0 666 1116 Size: 666 450 SacII CC_GC'GG Cuts at: 0 74 131 203 1116 Size: 74 57 72 913 Fragments arranged by size: 913 74 72 57 SfcI C'TryA_G Cuts at: 0 419 512 1116 Size: 419 93 604 Fragments arranged by size: 604 419 93 SfoI GGC'GCC Cuts at: 0 140 523 553 1116 Size: 140 383 30 563 Fragments arranged by size: 563 383 140 30 StyI C'CwwG_G Cuts at: 0 273 865 1055 1116 Size: 273 592 190 61 Fragments arranged by size: 592 273 190 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1107 1111 1116 Size: 1107 4 5 Fragments arranged by size: 1107 5 4 TatI w'GTAC_w Cuts at: 0 351 1116 Size: 351 765 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 476 508 939 971 1116 Size: 476 32 431 32 145 Fragments arranged by size: 476 431 145 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 102 1116 Size: 102 1014 Enzymes that do cut: AarI Acc65I AcuI AgeI AlwNI ApaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BtgI BtsI EaeI EagI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NotI PflMI PmlI PpuMI PsiI PspOMI PstI PvuII SacII SfcI SfoI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI MluI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PshAI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI