Restriction map for
pFN21AE0502
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MboII MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 223 CGCG CG^CG
AflIII 512 ACGTGT A^C[GA][CT]GT
BanI 503 GGCGCC G^G[CT][GA]CC
BbeI 503 GGCGCC GGCGC^C
BcgI 24 CGAACAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 91 CTAG C^TAG
BsaAI 511 TACGTG [CT]AC^GT[GA]
BsaI 53 GAGACC GGTCTC(1/5)
BsiEI 125 CGGCCG CG[GA][CT]^CG
BsmAI 53 GAGAC GTCTC(1/5)
BsrBI 520 GAGCGG CCGCTC(-3/-3)
BstUI 223 CGCG CG^CG
BstXI 173 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 214 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 125 CGGCCG C^GGCCG
EclXI 125 CGGCCG C^GGCCG
Eco52I 125 CGGCCG C^GGCCG
EcoO109I 214 AGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 503 GGCGCC [GA]GCGC^[CT]
HgaI 553 GACGC GACGC(5/10)
HphI 75 GGTGA GGTGA(8/7)
KasI 503 GGCGCC G^GCGCC
KspI 222 CCGCGG CCGC^GG
MaeI 91 CTAG C^TAG
MvnI 223 CGCG CG^CG
NarI 503 GGCGCC GG^CGCC
NcoI 48 CCATGG C^CATGG
PflMI 410 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 118 GAGCTC GAGCT^C
SacII 222 CCGCGG CCGC^GG
SfoI 503 GGCGCC GGC^GCC
SspI 322 AATATT AAT^ATT
Van91I 410 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 48 CCATGGAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 503 GGCGCC G[GA]^CG[CT]C
553 GACGCC
AspHI 17 GAGCAC G[AT]GC[AT]^C
118 GAGCTC
AvaII 275 GGTCC G^G[AT]CC
435 GGACC
BcnI 188 CCGGG CC^[GC]GG
490 CCCGG
BpmI 157 CTGGAG CTGGAG(16/14)
241 CTGGAG
BsaHI 503 GGCGCC G[GA]^CG[CT]C
553 GACGCC
BsiHKAI 17 GAGCAC G[AT]GC[AT]^C
118 GAGCTC
BsrI 539 ACTGG ACTGG(1/-1)
507 CCAGT
BstAPI 107 GCAAACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
573 GCAGTCTGTGC
CfoI 504 GCGC GCG^C
582 GCGC
DpnII 78 GATC ^GATC
337 GATC
EaeI 125 CGGCCG [CT]^GGCC[GA]
375 TGGCCG
HhaI 504 GCGC GCG^C
582 GCGC
Hin1I 503 GGCGCC G[GA]^CG[CT]C
553 GACGCC
HinP1I 504 GCGC G^CGC
582 GCGC
HinfI 184 GAGTC G^A[ACGT]TC
208 GAGTC
MaeIII 293 GTGAC ^GT[ACGT]AC
593 GTGAC
MboI 78 GATC ^GATC
337 GATC
MslI 174 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
538 CACTGGAATG
NciI 188 CCGGG CC^[GC]GG
490 CCCGG
NdeII 78 GATC ^GATC
337 GATC
NlaIII 49 CATG CATG^
424 CATG
PleI 184 GAGTC GAGTC(4/5)
208 GAGTC
PstI 32 CTGCAG CTGCA^G
571 CTGCAG
PvuII 35 CAGCTG CAG^CTG
568 CAGCTG
Sau3AI 78 GATC ^GATC
337 GATC
SfcI 32 CTGCAG C^T[GA][CT]AG
571 CTGCAG
Tsp45I 293 GTGAC ^GT[GC]AC
593 GTGAC
TspRI 372 CAGTG CA[GC]TG[ACGT][ACGT]^
538 CACTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DsaI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeII MnlI MspA1I MspI
MvaI MwoI NlaIV RsaI Sau96I
ScrFI SfaNI StyI TseI