Restriction map for
pFN21AE0501
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbsI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 571 CGCG CG^CG
AocI 288 CCTGAGG CC^T[ACGT]AGG
Asp700I 73 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 447 CACGTG CAC^GTG
BbvCI 279 CCTCAGC CCTCAGC(-5/-2)
BciVI 566 GTATCC GTATCC(6/5)
BfaI 157 CTAG C^TAG
BpmI 496 CTGGAG CTGGAG(16/14)
BsaAI 447 CACGTG [CT]AC^GT[GA]
BsmAI 506 GAGAC GTCTC(1/5)
BstF5I 327 CATCC GGATG(2/0)
BstUI 571 CGCG CG^CG
Bsu36I 288 CCTGAGG CC^T[ACGT]AGG
CfoI 561 GCGC GCG^C
Eco81I 288 CCTGAGG CC^T[ACGT]AGG
EcoNI 156 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 402 CCATGG C^C[AT][AT]GG
FokI 327 CATCC GGATG(9/13)
HhaI 561 GCGC GCG^C
HinP1I 561 GCGC G^CGC
MaeI 157 CTAG C^TAG
MspA1I 240 CCGCTG C[AC]G^C[GT]G
MvnI 571 CGCG CG^CG
NcoI 402 CCATGG C^CATGG
NspI 575 ACATGC [GA]CATG^[CT]
PmaCI 447 CACGTG CAC^GTG
PmlI 447 CACGTG CAC^GTG
PstI 484 CTGCAG CTGCA^G
SacI 97 GAGCTC GAGCT^C
SfcI 484 CTGCAG C^T[GA][CT]AG
StyI 402 CCATGG C^C[AT][AT]GG
TspRI 580 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 73 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 146 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
487 CAGTACCTG
ApaI 106 GGGCCC GGGCC^C
533 GGGCCC
AspHI 97 GAGCTC G[AT]GC[AT]^C
316 GAGCAC
AvaII 169 GGACC G^G[AT]CC
254 GGACC
BanI 584 GGCACC G^G[CT][GA]CC
595 GGTGCC
BbvI 260 GCAGC GCAGC(8/12)
521 GCAGC
BcnI 353 CCGGG CC^[GC]GG
469 CCCGG
BsaBI 54 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
363 GATGGAAATC
BsiHKAI 97 GAGCTC G[AT]GC[AT]^C
316 GAGCAC
BsmFI 245 GGGAC GGGAC(10/14)
39 GTCCC
BspMI 491 ACCTGC ACCTGC(4/8)
145 GCAGGT
BsrI 130 ACTGG ACTGG(1/-1)
462 CCAGT
BstAPI 486 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
552 GCAGTCCGTGC
BstXI 296 CCATCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
396 CCACGCCCATGG
DsaI 402 CCATGG C^C[GA][CT]GG
477 CCGTGG
Fnu4HI 260 GCAGC GC^[ACGT]GC
521 GCAGC
HapII 353 CCGG C^CGG
470 CCGG
HinfI 247 GACTC G^A[ACGT]TC
274 GACTC
HpaII 353 CCGG C^CGG
470 CCGG
ItaI 260 GCAGC GC^[ACGT]GC
521 GCAGC
MaeII 448 ACGT A^CGT
591 ACGT
MamI 54 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
363 GATGGAAATC
MboII 357 GAAGA GAAGA(8/7)
360 GAAGA
MspI 353 CCGG C^CGG
470 CCGG
NciI 353 CCGGG CC^[GC]GG
469 CCCGG
PleI 247 GACTC GAGTC(4/5)
274 GACTC
TseI 260 GCAGC G^C[AT]GC
521 GCAGC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI BanII BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
HaeIII HphI MboI MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI