Restriction map for
pFN21AE0501
(Linear) (Six-base) MAPSORT of: KIEE0501.seq Check: 1688 from: 1 to: 615
KIEE0501.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 615
Size: 142 473
AleI CACnn'nnGTG
Cuts at: 0 590 615
Size: 590 25
AlwNI CAG_nnn'CTG
Cuts at: 0 151 492 615
Size: 151 341 123
Fragments arranged by size:
341 151 123
ApaI G_GGCC'C
Cuts at: 0 110 537 615
Size: 110 427 78
Fragments arranged by size:
427 110 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 472 505 615
Size: 472 33 110
Fragments arranged by size:
472 110 33
BanI G'GyrC_C
Cuts at: 0 584 595 615
Size: 584 11 20
Fragments arranged by size:
584 20 11
BanII G_rGCy'C
Cuts at: 0 101 110 537 615
Size: 101 9 427 78
Fragments arranged by size:
427 101 78 9
BbvCI CC'TCA_GC
Cuts at: 0 280 615
Size: 280 335
BciVI GTATCCnnnnn_n'
Cuts at: 0 577 615
Size: 577 38
Bme1580I G_kGCm'C
Cuts at: 0 110 537 615
Size: 110 427 78
Fragments arranged by size:
427 110 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 615
Size: 517 98
Bpu10I CC'TnA_GC
Cuts at: 0 280 615
Size: 280 335
BsaAI yAC'GTr
Cuts at: 0 449 615
Size: 449 166
BsaBI GATnn'nnATC
Cuts at: 0 58 367 615
Size: 58 309 248
Fragments arranged by size:
309 248 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 615
Size: 406 30 179
Fragments arranged by size:
406 179 30
BseYI C'CCAG_C
Cuts at: 0 242 388 615
Size: 242 146 227
Fragments arranged by size:
242 227 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 457 571 615
Size: 181 276 114 44
Fragments arranged by size:
276 181 114 44
BsiHKAI G_wGCw'C
Cuts at: 0 101 320 615
Size: 101 219 295
Fragments arranged by size:
295 219 101
Bsp1286I G_dGCh'C
Cuts at: 0 101 110 320 537 615
Size: 101 9 210 217 78
Fragments arranged by size:
217 210 101 78 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 500 615
Size: 136 364 115
Fragments arranged by size:
364 136 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 558 615
Size: 492 66 57
Fragments arranged by size:
492 66 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 403 615
Size: 303 100 212
Fragments arranged by size:
303 212 100
Bsu36I CC'TnA_GG
Cuts at: 0 289 615
Size: 289 326
BtgI C'CryG_G
Cuts at: 0 402 477 615
Size: 402 75 138
Fragments arranged by size:
402 138 75
BtsI GCAGTG_nn'
Cuts at: 0 586 615
Size: 586 29
EcoICRI GAG'CTC
Cuts at: 0 99 615
Size: 99 516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 517 615
Size: 142 375 98
Fragments arranged by size:
375 142 98
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 615
Size: 160 455
EcoO109I rG'GnC_Cy
Cuts at: 0 107 533 534 615
Size: 107 426 1 81
Fragments arranged by size:
426 107 81 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 615
Size: 406 32 177
Fragments arranged by size:
406 177 32
MslI CAynn'nnrTG
Cuts at: 0 92 301 401 590 615
Size: 92 209 100 189 25
Fragments arranged by size:
209 189 100 92 25
MspA1I CmG'CkG
Cuts at: 0 242 615
Size: 242 373
NcoI C'CATG_G
Cuts at: 0 402 615
Size: 402 213
NspI r_CATG'y
Cuts at: 0 579 615
Size: 579 36
PasI CC'CwG_GG
Cuts at: 0 173 615
Size: 173 442
PmlI CAC'GTG
Cuts at: 0 449 615
Size: 449 166
PspOMI G'GGCC_C
Cuts at: 0 106 533 615
Size: 106 427 82
Fragments arranged by size:
427 106 82
PstI C_TGCA'G
Cuts at: 0 488 615
Size: 488 127
SacI G_AGCT'C
Cuts at: 0 101 615
Size: 101 514
SfcI C'TryA_G
Cuts at: 0 484 615
Size: 484 131
StyI C'CwwG_G
Cuts at: 0 402 615
Size: 402 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 615
Size: 165 450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 193 615
Size: 161 32 422
Fragments arranged by size:
422 161 32
XmnI GAAnn'nnTTC
Cuts at: 0 77 615
Size: 77 538
Enzymes that do cut:
AcuI AleI AlwNI ApaI BaeI BanI BanII BbvCI
BciVI Bme1580I BpmI Bpu10I BsaAI BsaBI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspMI BstAPI BstXI Bsu36I BtgI
BtsI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MslI MspA1I
NcoI NspI PasI PmlI PspOMI PstI SacI SfcI
StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BbsI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI