Restriction map for
pFN21AE0496
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspI AvrII BamHI BbeI BbrPI
BbvCI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
Hin1I HincII HindII HindIII HinfI
HpaI KasI Ksp632I MaeII MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 608 GGTACC G^GTACC
AccI 526 GTCTAC GT^[AC][GT]AC
AcsI 223 GAATTC [GA]^AATT[CT]
AhdI 296 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 388 AGCT AG^CT
AlwI 495 GATCC GGATC(4/5)
ApaI 74 GGGCCC GGGCC^C
ApoI 223 GAATTC [GA]^AATT[CT]
Asp718I 608 GGTACC G^GTACC
AspEI 296 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 272 TGCGCA TGC^GCA
BalI 737 TGGCCA TGG^CCA
BbsI 187 GTCTTC GAAGAC(2/6)
BciVI 591 GTATCC GTATCC(6/5)
BcnI 103 CCCGG CC^[GC]GG
BpuAI 187 GTCTTC GAAGAC(2/6)
BsaBI 336 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 561 GGTCTC GGTCTC(1/5)
BsiEI 264 CGACCG CG[GA][CT]^CG
BspMI 81 ACCTGC ACCTGC(4/8)
BstAPI 678 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 190 TTCGAA TT^CGAA
BstXI 422 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 494 AGATCC [GA]^GATC[CT]
Cac8I 692 GCCTGC GC[ACGT]^[ACGT]GC
DdeI 5 CTCAG C^T[ACGT]AG
Eam1105I 296 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 345 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 223 GAATTC G^AATTC
FspI 272 TGCGCA TGC^GCA
HaeII 142 AGCGCC [GA]GCGC^[CT]
KpnI 608 GGTACC GGTAC^C
MamI 336 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 188 TCTTC GAAGA(8/7)
MflI 494 AGATCC [GA]^GATC[CT]
MluNI 737 TGGCCA TGG^CCA
MscI 737 TGGCCA TGG^CCA
NciI 103 CCCGG CC^[GC]GG
NspV 190 TTCGAA TT^CGAA
PaeR7I 715 CTCGAG C^TCGAG
PflMI 515 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 83 CTGCAG CTGCA^G
SbfI 82 CCTGCAGG CCTGCA^GG
SfuI 190 TTCGAA TT^CGAA
SmlI 715 CTCGAG C^T[CT][GA]AG
Sse8387I 82 CCTGCAGG CCTGCA^GG
StuI 17 AGGCCT AGG^CCT
Tsp509I 224 AATT ^AATT
Van91I 515 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 715 CTCGAG C^TCGAG
XhoII 494 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 237 GTGCTC G[AT]GC[AT]^C
442 GAGCAC
AvaI 228 CTCGGG C^[CT]CG[GA]G
715 CTCGAG
AvaII 295 GGACC G^G[AT]CC
722 GGTCC
BanI 608 GGTACC G^G[CT][GA]CC
710 GGCACC
BanII 74 GGGCCC G[GA]GC[CT]^C
745 GAGCCC
BcgI 13 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
220 GCAGAATTCTCG
BfaI 98 CTAG C^TAG
622 CTAG
BglI 31 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
145 GCCAAGGCGGC
BpmI 564 CTCCAG CTGGAG(16/14)
346 CTGGAG
BsiHKAI 237 GTGCTC G[AT]GC[AT]^C
442 GAGCAC
BsoBI 228 CTCGGG C^[CT]CG[GA]G
715 CTCGAG
CfoI 143 GCGC GCG^C
273 GCGC
DpnII 400 GATC ^GATC
495 GATC
EaeI 29 CGGCCA [CT]^GGCC[GA]
737 TGGCCA
EcoT14I 62 CCAAGG C^C[AT][AT]GG
146 CCAAGG
HgaI 508 GACGC GACGC(5/10)
687 GCGTC
HhaI 143 GCGC GCG^C
273 GCGC
HinP1I 143 GCGC G^CGC
273 GCGC
HphI 42 GGTGA GGTGA(8/7)
631 GGTGA
MaeI 98 CTAG C^TAG
622 CTAG
MboI 400 GATC ^GATC
495 GATC
MslI 423 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
643 CACTGGAATG
NdeII 400 GATC ^GATC
495 GATC
PpuMI 294 GGGACCC [GA]G^G[AT]CC[CT]
721 GGGTCCT
Sau3AI 400 GATC ^GATC
495 GATC
SfcI 83 CTGCAG C^T[GA][CT]AG
174 CTACAG
StyI 62 CCAAGG C^C[AT][AT]GG
146 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AlwNI BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cfr13I DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII ItaI KspI
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NlaIII NlaIV RsaI
SacII Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I