(Circular) (Six-base) MAPSORT of: pFN21AE0496.seq Check: 6212 from: 1 to: 5461 pFN21AE0496 5461 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2095 2095 Size: 5461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2532 2804 1937 Size: 595 272 4594 Fragments arranged by size: 4594 595 272 Acc65I G'GTAC_C Cuts at: 2613 2782 2613 Size: 169 5292 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2524 3524 4908 5176 1632 Size: 892 1000 1384 268 1917 Fragments arranged by size: 1917 1384 1000 892 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 2306 4190 2306 Size: 1884 3577 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5461 AlwNI CAG_nnn'CTG Cuts at: 2060 2526 2689 3204 4776 2060 Size: 466 163 515 1572 2745 Fragments arranged by size: 2745 1572 515 466 163 ApaI G_GGCC'C Cuts at: 2083 2083 Size: 5461 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2228 2770 2996 3148 5257 1493 Size: 735 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 735 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2233 2720 2786 1870 Size: 73 290 487 66 4545 Fragments arranged by size: 4545 487 290 73 66 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2613 2715 2782 4237 618 Size: 325 217 627 826 102 67 1455 1842 Fragments arranged by size: 1842 1455 826 627 325 217 102 67 BanII G_rGCy'C Cuts at: 1391 2083 2754 2780 1391 Size: 692 671 26 4072 Fragments arranged by size: 4072 692 671 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 928 Size: 971 286 4204 Fragments arranged by size: 4204 971 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2007 2041 2214 2248 3652 3686 1903 Size: 34 70 34 173 34 1404 34 3678 Fragments arranged by size: 3678 1404 173 70 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2607 3353 4563 2607 Size: 746 1210 3505 Fragments arranged by size: 3505 1210 746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 2156 4072 Size: 107 122 71 631 605 370 114 1916 Cuts at: 4072 136 Size: 1525 Fragments arranged by size: 1916 1525 631 605 370 122 114 107 71 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2083 3522 4678 1790 Size: 293 1439 1156 2573 Fragments arranged by size: 2573 1439 1156 293 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2514 3192 4150 Size: 416 53 83 399 24 1097 678 958 Cuts at: 4150 442 Size: 1753 Fragments arranged by size: 1753 1097 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2554 4121 1239 Size: 1133 182 1567 2579 Fragments arranged by size: 2579 1567 1133 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2572 4124 882 Size: 617 1073 1552 2219 Fragments arranged by size: 2219 1552 1073 617 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 2345 5455 2345 Size: 3110 2351 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3268 3650 4285 275 Size: 53 83 186 936 1735 382 635 1451 Fragments arranged by size: 1735 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3894 4292 4566 4713 5124 3894 Size: 398 274 147 411 4231 Fragments arranged by size: 4231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 4168 Fragments arranged by size: 4168 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 4664 5160 4664 Size: 496 4965 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4888 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2272 2911 3672 4700 1540 Size: 275 186 271 639 761 1028 2301 Fragments arranged by size: 2301 1028 761 639 275 271 186 BsiHKAI G_wGCw'C Cuts at: 2246 2451 3522 3607 4678 2246 Size: 205 1071 85 1071 3029 Fragments arranged by size: 3029 1071 1071 205 85 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2246 2451 2754 2780 3522 3607 Size: 399 293 163 205 303 26 742 85 Cuts at: 3607 4678 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 293 205 163 85 26 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2816 844 Size: 1251 721 3489 Fragments arranged by size: 3489 1251 721 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4105 4292 1700 Size: 110 2295 187 2869 Fragments arranged by size: 2869 2295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 794 Size: 1895 3566 BstBI TT'CG_AA Cuts at: 2196 4265 2196 Size: 2069 3392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2499 2791 3158 3543 3560 5012 5098 5110 Size: 527 292 367 385 17 1452 86 12 Cuts at: 5110 5456 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 527 385 367 346 292 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2260 2295 2332 2608 513 Size: 552 1195 35 37 276 3366 Fragments arranged by size: 3366 1195 552 276 37 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2407 2717 2977 3771 3791 2407 Size: 310 260 794 20 4077 Fragments arranged by size: 4077 794 310 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2742 2908 Size: 54 1425 50 275 18 204 708 166 Cuts at: 2908 3797 5426 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 708 275 204 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3391 1353 Size: 2038 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4048 4422 4568 387 Size: 1064 138 585 1874 374 146 1280 Fragments arranged by size: 1874 1280 1064 585 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2524 2554 3524 4121 4908 5176 Size: 393 740 152 30 970 597 787 268 Cuts at: 5176 1239 Size: 1524 Fragments arranged by size: 1524 970 787 740 597 393 268 152 30 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 5461 EcoO109I rG'GnC_Cy Cuts at: 2057 2079 2080 2300 2727 2057 Size: 22 1 220 427 4791 Fragments arranged by size: 4791 427 220 22 1 EcoRI G'AATT_C Cuts at: 2228 2770 3148 2228 Size: 542 378 4541 Fragments arranged by size: 4541 542 378 FspI TGC'GCA Cuts at: 2279 3967 2279 Size: 1688 3773 HaeII r_GCGC'y Cuts at: 1678 2151 4608 1678 Size: 473 2457 2531 Fragments arranged by size: 2531 2457 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2292 2324 4102 4134 4176 Size: 32 135 32 720 32 1778 32 42 Cuts at: 4176 4208 1373 Size: 32 2626 Fragments arranged by size: 2626 1778 720 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KpnI G_GTAC'C Cuts at: 2617 2786 2617 Size: 169 5292 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 4575 4759 1305 Size: 1044 2226 184 2007 Fragments arranged by size: 2226 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2744 10 Size: 54 1425 343 912 2727 Fragments arranged by size: 2727 1425 912 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2652 3419 3778 3937 518 Size: 648 1266 220 767 359 159 2042 Fragments arranged by size: 2042 1266 767 648 359 220 159 MspA1I CmG'CkG Cuts at: 1606 2297 2334 2610 2877 3554 4702 4947 1606 Size: 691 37 276 267 677 1148 245 2120 Fragments arranged by size: 2120 1148 691 677 276 267 245 37 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4909 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2819 4364 1132 Size: 97 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 2054 2304 1348 Size: 706 250 4505 Fragments arranged by size: 4505 706 250 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 2526 1108 Size: 1418 4043 PfoI T'CCnGG_A Cuts at: 2349 2349 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2300 2727 2300 Size: 427 5034 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PspOMI G'GGCC_C Cuts at: 2079 2079 Size: 5461 PspXI vC'TCGA_Gb Cuts at: 2720 2720 Size: 5461 PstI C_TGCA'G Cuts at: 830 2092 2813 830 Size: 1262 721 3478 Fragments arranged by size: 3478 1262 721 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SacII CC_GC'GG Cuts at: 2298 2335 2611 2298 Size: 37 276 5148 Fragments arranged by size: 5148 276 37 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SanDI GG'GwC_CC Cuts at: 2300 2300 Size: 5461 SbfI CC_TGCA'GG Cuts at: 2092 2813 2092 Size: 721 4740 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2088 2179 2809 3944 4625 4816 826 Size: 219 1043 91 630 1135 681 191 1471 Fragments arranged by size: 1471 1135 1043 681 630 219 191 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2720 3565 4466 4728 820 Size: 197 926 777 845 901 262 1553 Fragments arranged by size: 1553 926 901 845 777 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 2024 1517 Size: 507 4954 StyI C'CwwG_G Cuts at: 513 1065 2067 2151 2757 2900 513 Size: 552 1002 84 606 143 3074 Fragments arranged by size: 3074 1002 606 552 143 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2533 3488 3505 3658 3843 4288 325 Size: 1255 953 955 17 153 185 445 1498 Fragments arranged by size: 1498 1255 955 953 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2441 2473 5421 5453 2441 Size: 32 2948 32 2449 Fragments arranged by size: 2948 2449 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 2720 1943 Size: 777 4684 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI