Restriction map for
pFN21AE0496
(Linear) (Six-base) MAPSORT of: KIEE0496.seq Check: 9572 from: 1 to: 756
KIEE0496.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 756
Size: 90 666
AccI GT'mk_AC
Cuts at: 0 527 756
Size: 527 229
Acc65I G'GTAC_C
Cuts at: 0 608 756
Size: 608 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 756
Size: 519 237
AhdI GACnn_n'nnGTC
Cuts at: 0 301 756
Size: 301 455
AlwNI CAG_nnn'CTG
Cuts at: 0 55 521 684 756
Size: 55 466 163 72
Fragments arranged by size:
466 163 72 55
ApaI G_GGCC'C
Cuts at: 0 78 756
Size: 78 678
ApoI r'AATT_y
Cuts at: 0 223 756
Size: 223 533
AvaI C'yCGr_G
Cuts at: 0 228 715 756
Size: 228 487 41
Fragments arranged by size:
487 228 41
BanI G'GyrC_C
Cuts at: 0 608 710 756
Size: 608 102 46
Fragments arranged by size:
608 102 46
BanII G_rGCy'C
Cuts at: 0 78 749 756
Size: 78 671 7
Fragments arranged by size:
671 78 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 756
Size: 180 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2 36 209 243 756
Size: 2 34 173 34 513
Fragments arranged by size:
513 173 34 34 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 602 756
Size: 602 154
BglI GCCn_nnn'nGGC
Cuts at: 0 37 151 756
Size: 37 114 605
Fragments arranged by size:
605 114 37
Bme1580I G_kGCm'C
Cuts at: 0 78 756
Size: 78 678
BmrI ACTGGGnnnn_n'
Cuts at: 0 509 756
Size: 509 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 549 756
Size: 367 182 207
Fragments arranged by size:
367 207 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 567 756
Size: 567 189
BsaBI GATnn'nnATC
Cuts at: 0 340 756
Size: 340 416
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 756
Size: 436 30 290
Fragments arranged by size:
436 290 30
BsiEI CG_ry'CG
Cuts at: 0 267 756
Size: 267 489
BsiHKAI G_wGCw'C
Cuts at: 0 241 446 756
Size: 241 205 310
Fragments arranged by size:
310 241 205
Bsp1286I G_dGCh'C
Cuts at: 0 78 241 446 749 756
Size: 78 163 205 303 7
Fragments arranged by size:
303 205 163 78 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 756
Size: 90 666
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 756
Size: 684 72
BstBI TT'CG_AA
Cuts at: 0 191 756
Size: 191 565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 756
Size: 429 327
BstYI r'GATC_y
Cuts at: 0 494 756
Size: 494 262
BtgI C'CryG_G
Cuts at: 0 255 290 327 603 756
Size: 255 35 37 276 153
Fragments arranged by size:
276 255 153 37 35
BtsI GCAGTG_nn'
Cuts at: 0 402 712 756
Size: 402 310 44
Fragments arranged by size:
402 310 44
EaeI y'GGCC_r
Cuts at: 0 29 737 756
Size: 29 708 19
Fragments arranged by size:
708 29 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 756
Size: 169 587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 519 549 756
Size: 367 152 30 207
Fragments arranged by size:
367 207 152 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 756
Size: 349 407
EcoO109I rG'GnC_Cy
Cuts at: 0 52 74 75 295 722 756
Size: 52 22 1 220 427 34
Fragments arranged by size:
427 220 52 34 22 1
EcoRI G'AATT_C
Cuts at: 0 223 756
Size: 223 533
FspI TGC'GCA
Cuts at: 0 274 756
Size: 274 482
HaeII r_GCGC'y
Cuts at: 0 146 756
Size: 146 610
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 756
Size: 287 32 437
Fragments arranged by size:
437 287 32
KpnI G_GTAC'C
Cuts at: 0 612 756
Size: 612 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 756
Size: 344 412
MscI TGG'CCA
Cuts at: 0 739 756
Size: 739 17
MslI CAynn'nnrTG
Cuts at: 0 427 647 756
Size: 427 220 109
Fragments arranged by size:
427 220 109
MspA1I CmG'CkG
Cuts at: 0 292 329 605 756
Size: 292 37 276 151
Fragments arranged by size:
292 276 151 37
PasI CC'CwG_GG
Cuts at: 0 49 299 756
Size: 49 250 457
Fragments arranged by size:
457 250 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 521 756
Size: 521 235
PfoI T'CCnGG_A
Cuts at: 0 344 756
Size: 344 412
PpuMI rG'GwC_Cy
Cuts at: 0 295 722 756
Size: 295 427 34
Fragments arranged by size:
427 295 34
PspOMI G'GGCC_C
Cuts at: 0 74 756
Size: 74 682
PspXI vC'TCGA_Gb
Cuts at: 0 715 756
Size: 715 41
PstI C_TGCA'G
Cuts at: 0 87 756
Size: 87 669
SacII CC_GC'GG
Cuts at: 0 293 330 606 756
Size: 293 37 276 150
Fragments arranged by size:
293 276 150 37
SanDI GG'GwC_CC
Cuts at: 0 295 756
Size: 295 461
SbfI CC_TGCA'GG
Cuts at: 0 87 756
Size: 87 669
SfcI C'TryA_G
Cuts at: 0 83 174 756
Size: 83 91 582
Fragments arranged by size:
582 91 83
SmlI C'TyrA_G
Cuts at: 0 715 756
Size: 715 41
StuI AGG'CCT
Cuts at: 0 19 756
Size: 19 737
StyI C'CwwG_G
Cuts at: 0 62 146 756
Size: 62 84 610
Fragments arranged by size:
610 84 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 528 756
Size: 528 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 756
Size: 436 32 288
Fragments arranged by size:
436 288 32
XhoI C'TCGA_G
Cuts at: 0 715 756
Size: 715 41
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BciVI BglI Bme1580I
BmrI BpmI BsaI BsaBI BsaXI BsiEI BsiHKAI Bsp1286I
BspMI BstAPI BstBI BstXI BstYI BtgI BtsI EaeI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I PasI PflMI PfoI
PpuMI PspOMI PspXI PstI SacII SanDI SbfI SfcI
SmlI StuI StyI TaqII TstI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XmaI XmnI ZraI