(Circular) (Six-base) MAPSORT of: pFN21AE0001.seq Check: 2907 from: 1 to: 6196 pFN21AE0001 6196 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2549 2549 Size: 6196 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2586 3539 1937 Size: 649 953 4594 Fragments arranged by size: 4594 953 649 Acc65I G'GTAC_C Cuts at: 2919 3517 2919 Size: 598 5598 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 4259 5643 5911 1632 Size: 1167 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 1167 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 2909 5095 6072 1203 Size: 1706 2186 977 1327 Fragments arranged by size: 2186 1706 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3241 1184 Size: 2057 4139 AlwNI CAG_nnn'CTG Cuts at: 2344 3939 5511 2344 Size: 1595 1572 3029 Fragments arranged by size: 3029 1595 1572 ApaI G_GGCC'C Cuts at: 2313 2313 Size: 6196 ApaLI G'TGCA_C Cuts at: 2479 3335 4253 5409 2479 Size: 856 918 1156 3266 Fragments arranged by size: 3266 1156 918 856 ApoI r'AATT_y Cuts at: 1493 3505 3731 3883 5992 1493 Size: 2012 226 152 2109 1697 Fragments arranged by size: 2109 2012 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2431 2467 3456 3521 1870 Size: 73 196 292 36 989 65 4545 Fragments arranged by size: 4545 989 292 196 73 65 36 BamHI G'GATC_C Cuts at: 2060 3021 3526 3893 2060 Size: 961 505 367 4363 Fragments arranged by size: 4363 961 505 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2919 3517 4972 618 Size: 325 217 627 1132 598 1455 1842 Fragments arranged by size: 1842 1455 1132 627 598 325 217 BanII G_rGCy'C Cuts at: 1391 2313 3280 3416 3515 1391 Size: 922 967 136 99 4072 Fragments arranged by size: 4072 967 922 136 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3407 928 Size: 971 1508 3717 Fragments arranged by size: 3717 1508 971 BbvCI CC'TCA_GC Cuts at: 3113 3113 Size: 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2934 4807 136 Size: 107 122 71 631 605 1262 1873 1525 Fragments arranged by size: 1873 1525 1262 631 605 122 107 71 BglII A'GATC_T Cuts at: 2958 6191 2958 Size: 3233 2963 BlpI GC'TnA_GC Cuts at: 2108 3192 3613 2108 Size: 1084 421 4691 Fragments arranged by size: 4691 1084 421 Bme1580I G_kGCm'C Cuts at: 1790 2313 2483 2669 2930 3203 3339 4257 5413 Size: 523 170 186 261 273 136 918 1156 Cuts at: 5413 1790 Size: 2573 Fragments arranged by size: 2573 1156 918 523 273 261 186 170 136 BmgBI CAC'GTC Cuts at: 1318 3373 3379 1318 Size: 2055 6 4135 Fragments arranged by size: 4135 2055 6 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2365 3283 3927 Size: 416 53 83 399 24 948 918 644 Cuts at: 3927 4885 442 Size: 958 1753 Fragments arranged by size: 1753 958 948 918 644 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2482 2152 Size: 330 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 4856 1239 Size: 1043 2574 2579 Fragments arranged by size: 2579 2574 1043 Bpu10I CC'TnA_GC Cuts at: 3113 3419 3113 Size: 306 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3298 4321 5186 5484 3298 Size: 1023 865 298 4010 Fragments arranged by size: 4010 1023 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2809 4859 882 Size: 617 1310 2050 2219 Fragments arranged by size: 2219 2050 1310 617 BsaAI yAC'GTr Cuts at: 493 1204 2910 3409 3987 493 Size: 711 1706 499 578 2702 Fragments arranged by size: 2702 1706 711 578 499 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3280 3474 4629 5027 5301 5448 5859 3280 Size: 194 1155 398 274 147 411 3617 Fragments arranged by size: 3617 1155 411 398 274 194 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2710 2740 3030 3060 1178 Size: 30 165 30 1307 30 290 30 4314 Fragments arranged by size: 4314 1307 290 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2189 3444 1186 Size: 928 75 1255 3938 Fragments arranged by size: 3938 1255 928 75 BseYI C'CCAG_C Cuts at: 2672 2929 5399 5895 2672 Size: 257 2470 496 2973 Fragments arranged by size: 2973 2470 496 257 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2234 2408 3125 3182 1163 Size: 573 438 60 174 717 57 4177 Fragments arranged by size: 4177 717 573 438 174 60 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3186 3646 4407 5435 1540 Size: 275 186 1185 460 761 1028 2301 Fragments arranged by size: 2301 1185 1028 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2468 2483 3339 4257 4342 5413 2307 Size: 161 15 856 918 85 1071 3090 Fragments arranged by size: 3090 1071 918 856 161 85 15 BsmI GAATG_Cn' Cuts at: 3707 3800 3707 Size: 93 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2313 2468 2483 2669 2930 3203 Size: 399 517 6 155 15 186 261 273 Cuts at: 3203 3280 3339 3416 3515 4257 4342 5413 1391 Size: 77 59 77 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 273 261 186 155 99 85 77 77 59 15 6 BspEI T'CCGG_A Cuts at: 3474 5859 3474 Size: 2385 3811 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2549 3484 3551 844 Size: 1508 197 935 67 3489 Fragments arranged by size: 3489 1508 935 197 67 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4691 4865 65 Size: 1183 3443 174 1396 Fragments arranged by size: 3443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2649 4840 5027 1700 Size: 110 839 2191 187 2869 Fragments arranged by size: 2869 2191 839 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6196 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstXI CCAn_nnnn'nTGG Cuts at: 2323 2323 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2060 2178 2958 3021 3477 3526 3893 4278 Size: 88 118 780 63 456 49 367 385 Cuts at: 4278 4295 5747 5833 5845 6191 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 456 385 367 346 118 88 86 63 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2220 2220 Size: 6196 BtgI C'CryG_G Cuts at: 513 1065 3387 513 Size: 552 2322 3322 Fragments arranged by size: 3322 2322 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3223 3250 3712 4506 4526 3223 Size: 27 462 794 20 4893 Fragments arranged by size: 4893 794 462 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3343 3378 529 Size: 35 2779 35 3347 Fragments arranged by size: 3347 2779 35 35 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DraIII CAC_nnn'GTG Cuts at: 3103 3103 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2647 3643 4532 Size: 54 1425 50 275 18 817 996 889 Cuts at: 4532 6161 8 Size: 1629 43 Fragments arranged by size: 1629 1425 996 889 817 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4126 1353 Size: 2773 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2799 4259 4856 5643 5911 1239 Size: 393 650 517 1460 597 787 268 1524 Fragments arranged by size: 1524 1460 787 650 597 517 393 268 EcoO109I rG'GnC_Cy Cuts at: 2471 3070 2471 Size: 599 5597 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 EcoRV GAT'ATC Cuts at: 3052 3052 Size: 6196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3091 3091 Size: 6196 FseI GG_CCGG'CC Cuts at: 2653 2653 Size: 6196 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 2352 5343 1678 Size: 674 2991 2531 Fragments arranged by size: 2991 2531 674 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3175 3207 4837 4869 4911 Size: 32 135 32 1603 32 1630 32 42 Cuts at: 4911 4943 1373 Size: 32 2626 Fragments arranged by size: 2626 1630 1603 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2848 3540 3786 669 Size: 988 281 910 692 246 3079 Fragments arranged by size: 3079 988 910 692 281 246 HindIII A'AGCT_T Cuts at: 748 2198 748 Size: 1450 4746 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 2923 3521 2923 Size: 598 5598 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 2824 5310 5494 1305 Size: 948 571 2486 184 2007 Fragments arranged by size: 2486 2007 948 571 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4374 Fragments arranged by size: 4374 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2882 4154 4513 4672 518 Size: 648 1716 1272 359 159 2042 Fragments arranged by size: 2042 1716 1272 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2688 3196 3612 4289 5437 5682 1606 Size: 1082 508 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 1082 677 508 416 245 NaeI GCC'GGC Cuts at: 2651 2651 Size: 6196 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5644 NdeI CA'TA_TG Cuts at: 387 2996 387 Size: 2609 3587 NgoMIV G'CCGG_C Cuts at: 2649 2649 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 3554 5099 1132 Size: 97 2325 1545 2229 Fragments arranged by size: 2325 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2160 1348 Size: 812 5384 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PfoI T'CCnGG_A Cuts at: 3024 3024 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PmlI CAC'GTG Cuts at: 2910 2910 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3070 3070 Size: 6196 PshAI GACnn'nnGTC Cuts at: 1613 2861 1613 Size: 1248 4948 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PspOMI G'GGCC_C Cuts at: 2309 2309 Size: 6196 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2570 2602 2570 Size: 32 6164 PstI C_TGCA'G Cuts at: 830 2278 2693 3548 830 Size: 1448 415 855 3478 Fragments arranged by size: 3478 1448 855 415 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 2688 3196 2688 Size: 508 5688 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2274 2689 3544 4679 5360 5551 826 Size: 219 1229 415 855 1135 681 191 1471 Fragments arranged by size: 1471 1229 1135 855 681 415 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 2141 3523 1872 Size: 269 1382 4545 Fragments arranged by size: 4545 1382 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 3313 4300 5201 5463 820 Size: 197 926 1370 987 901 262 1553 Fragments arranged by size: 1553 1370 987 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 2225 3042 3470 1517 Size: 708 817 428 4243 Fragments arranged by size: 4243 817 708 428 StyI C'CwwG_G Cuts at: 513 1065 2034 2065 3635 513 Size: 552 969 31 1570 3074 Fragments arranged by size: 3074 1570 969 552 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2009 2686 4223 4240 4393 4578 5023 Size: 1255 429 677 1537 17 153 185 445 Cuts at: 5023 325 Size: 1498 Fragments arranged by size: 1537 1498 1255 677 445 429 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 4442 6154 Size: 274 80 33 51 1443 261 2204 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 2204 1712 1443 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3030 3062 6156 6188 3030 Size: 32 3094 32 3038 Fragments arranged by size: 3094 3038 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2616 3139 2320 Size: 296 523 5377 Fragments arranged by size: 5377 523 296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 2139 3521 1870 Size: 269 1382 4545 Fragments arranged by size: 4545 1382 269 XmnI GAAnn'nnTTC Cuts at: 4325 4325 Size: 6196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI