Restriction map for
pFN21AE0430
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 730 GTAGAC GT^[AC][GT]AC
AcsI 234 GAATTT [GA]^AATT[CT]
Alw44I 1127 GTGCAC G^TGCAC
AlwI 1041 GATCC GGATC(4/5)
AocI 670 CCTGAGG CC^T[ACGT]AGG
ApaLI 1127 GTGCAC G^TGCAC
ApoI 234 GAATTT [GA]^AATT[CT]
AvrII 834 CCTAGG C^CTAGG
BanI 436 GGCACC G^G[CT][GA]CC
BlnI 834 CCTAGG C^CTAGG
BsaI 259 GAGACC GGTCTC(1/5)
BseRI 337 GAGGAG GAGGAG(10/8)
BsgI 291 GTGCAG GTGCAG(16/14)
BsrDI 690 GCAATG GCAATG(2/0)
BsrFI 553 GCCGGC [GA]^CCGG[CT]
BssSI 521 CACGAG CACGAG(-5/-1)
BstXI 617 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1040 AGATCC [GA]^GATC[CT]
Bsu36I 670 CCTGAGG CC^T[ACGT]AGG
CfoI 1221 GCGC GCG^C
Cfr10I 553 GCCGGC [GA]^CCGG[CT]
DpnII 1041 GATC ^GATC
DsaI 7 CCATGG C^C[GA][CT]GG
Eco81I 670 CCTGAGG CC^T[ACGT]AGG
EcoNI 267 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 1221 GCGC GCG^C
HinP1I 1221 GCGC G^CGC
HincII 157 GTTGAC GT[CT]^[GA]AC
HindII 157 GTTGAC GT[CT]^[GA]AC
MboI 1041 GATC ^GATC
MflI 1040 AGATCC [GA]^GATC[CT]
MseI 167 TTAA T^TAA
MslI 1023 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 553 GCCGGC GCC^GGC
NcoI 7 CCATGG C^CATGG
NdeII 1041 GATC ^GATC
NgoMIV 553 GCCGGC G^CCGGC
PleI 1048 GAGTC GAGTC(4/5)
PstI 1096 CTGCAG CTGCA^G
PvuII 32 CAGCTG CAG^CTG
Sau3AI 1041 GATC ^GATC
SfcI 1096 CTGCAG C^T[GA][CT]AG
SfiI 577 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 721 CTCAAG C^T[CT][GA]AG
Tru9I 167 TTAA T^TAA
XhoII 1040 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 394 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
665 CAGGACCTG
BalI 576 TGGCCA TGG^CCA
1133 TGGCCA
BbsI 1078 GAAGAC GAAGAC(2/6)
108 GTCTTC
BbvCI 48 CCTCAGC CCTCAGC(-5/-2)
121 CCTCAGC
BfaI 25 CTAG C^TAG
835 CTAG
BpuAI 1078 GAAGAC GAAGAC(2/6)
108 GTCTTC
BspMI 142 ACCTGC ACCTGC(4/8)
891 GCAGGT
MaeI 25 CTAG C^TAG
835 CTAG
MaeIII 542 GTGAC ^GT[ACGT]AC
1226 GTGAC
MluNI 576 TGGCCA TGG^CCA
1133 TGGCCA
MscI 576 TGGCCA TGG^CCA
1133 TGGCCA
MspA1I 32 CAGCTG C[AC]G^C[GT]G
531 CAGCGG
PpuMI 72 AGGACCT [GA]G^G[AT]CC[CT]
666 AGGACCT
StuI 569 AGGCCT AGG^CCT
827 AGGCCT
TaqI 308 TCGA T^CGA
911 TCGA
TfiI 409 GATTC G^A[AT]TC
676 GAATC
Tsp45I 542 GTGAC ^GT[GC]AC
1226 GTGAC
Tsp509I 235 AATT ^AATT
779 AATT
TthHB8I 308 TCGA T^CGA
911 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI StyI TseI TspRI
XcmI