Restriction map for
pFN21AE0430
(Circular) (Six-base) MAPSORT of: pFN21AE0430.seq Check: 6731 from: 1 to: 5956
pFN21AE0430 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2887 2887
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2736 3299 1937
Size: 799 563 4594
Fragments arranged by size:
4594 799 563
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 4019 5403 5671 1632
Size: 809 1578 1384 268 1917
Fragments arranged by size:
1917 1578 1384 809 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 2404 2675 3699 5271 2404
Size: 271 1024 1572 3089
Fragments arranged by size:
3089 1572 1024 271
ApaI G_GGCC'C
Cuts at: 2386 2454 2716 2386
Size: 68 262 5626
Fragments arranged by size:
5626 262 68
ApaLI G'TGCA_C
Cuts at: 3132 4013 5169 3132
Size: 881 1156 3919
Fragments arranged by size:
3919 1156 881
ApoI r'AATT_y
Cuts at: 1493 2239 3265 3491 3643 5752 1493
Size: 746 1026 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
AvrII C'CTAG_G
Cuts at: 2839 2839
Size: 5956
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 3277 4732 618
Size: 325 217 627 654 836 1455 1842
Fragments arranged by size:
1842 1455 836 654 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2386 2454 2716 2807 2906 3275 1391
Size: 995 68 262 91 99 369 4072
Fragments arranged by size:
4072 995 369 262 99 91 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 3090 928
Size: 971 207 984 3794
Fragments arranged by size:
3794 984 971 207
BbvCI CC'TCA_GC
Cuts at: 2054 2127 2054
Size: 73 5883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 2559 2589
Size: 107 122 71 631 605 767 120 30
Cuts at: 2589 3146 4567 136
Size: 557 1421 1525
Fragments arranged by size:
1525 1421 767 631 605 557 122 120
107 71 30
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2386 2454 2544 2662 2716 3136 4017 5173
Size: 596 68 90 118 54 420 881 1156
Cuts at: 5173 1790
Size: 2573
Fragments arranged by size:
2573 1156 881 596 420 118 90 68
54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 3687 4645
Size: 416 53 83 399 24 687 1583 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1583 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2204 2306 2351 3263 4616 1239
Size: 836 129 102 45 912 1353 2579
Fragments arranged by size:
2579 1353 912 836 129 102 45
Bpu10I CC'TnA_GC
Cuts at: 2054 2127 2668 3153 2054
Size: 73 541 485 4857
Fragments arranged by size:
4857 541 485 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 4081 4946 5244 2711
Size: 1370 865 298 3423
Fragments arranged by size:
3423 1370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4619 882
Size: 617 759 2361 2219
Fragments arranged by size:
2361 2219 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 2345 2375 1178
Size: 30 165 30 876 30 36 30 4759
Fragments arranged by size:
4759 876 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 1186
Size: 1171 4785
BseYI C'CCAG_C
Cuts at: 2035 2709 5159 5655 2035
Size: 674 2450 496 2336
Fragments arranged by size:
2450 2336 674 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2317 1163
Size: 573 581 4802
Fragments arranged by size:
4802 581 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2411 3136 4017 4102 5173 2046
Size: 365 725 881 85 1071 2829
Fragments arranged by size:
2829 1071 881 725 365 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2386 2411 2454 2544 2662 2716
Size: 399 256 340 25 43 90 118 54
Cuts at: 2716 2807 2906 3136 3275 4017 4102 5173 1391
Size: 91 99 230 139 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 256 230 139
118 99 91 90 85 54 43 25
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2156 2887 3311 844
Size: 1312 731 424 3489
Fragments arranged by size:
3489 1312 731 424
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2702 4451 4625 65
Size: 1183 1454 1749 174 1396
Fragments arranged by size:
1749 1454 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2558 4600 4787 1700
Size: 110 748 2042 187 2869
Fragments arranged by size:
2869 2042 748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2526 4016 5028 2526
Size: 1490 1012 3454
Fragments arranged by size:
3454 1490 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 2629
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 3045 3286 3653 4038 4055 5507 5593 5605
Size: 1073 241 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1073 385 367 346 241 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2676
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 4457
Fragments arranged by size:
4457 939 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 2513 2969 3235 3472 4266 4286 2220
Size: 293 456 266 237 794 20 3890
Fragments arranged by size:
3890 794 456 293 266 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 3138 3198
Size: 54 1425 50 275 18 751 557 60
Cuts at: 3198 3403 4292 5921 8
Size: 205 889 1629 43
Fragments arranged by size:
1629 1425 889 751 557 275 205 60
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2229 2399 3886 1353
Size: 713 163 170 1487 3423
Fragments arranged by size:
3423 1487 713 170 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2204 2306 2351 2441 3263 4019
Size: 393 443 129 102 45 90 822 756
Cuts at: 4019 4616 5403 5671 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 822 787 756 597 443 393 268
129 102 90 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2276
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2026 2050 2078 2179 2382 2383 2451 2487 2672
Size: 24 28 101 203 1 68 36 185
Cuts at: 2672 2713 2026
Size: 41 5269
Fragments arranged by size:
5269 203 185 101 68 41 36 28
24 1
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 3178 2326
Size: 852 5104
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4597 4629 4671
Size: 32 135 32 656 32 2337 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 2337 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 3300 3546 669
Size: 988 281 226 1136 246 3079
Fragments arranged by size:
3079 1136 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2583 3140 10
Size: 54 1425 343 751 557 2826
Fragments arranged by size:
2826 1425 751 557 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3032 3914 4273 4432 518
Size: 648 1866 882 359 159 2042
Fragments arranged by size:
2042 1866 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2538 3372 4049 5197 5442 1606
Size: 433 499 834 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 499 433 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 4457
Fragments arranged by size:
4457 939 552 8
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 2196 2947 2196
Size: 751 5205
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2078 2672 2078
Size: 594 5362
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2382 2450 2712 2382
Size: 68 262 5626
Fragments arranged by size:
5626 262 68
PstI C_TGCA'G
Cuts at: 830 3105 3308 830
Size: 2275 203 3478
Fragments arranged by size:
3478 2275 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2039 2039
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3101 3304 4439 5120 5311 826
Size: 219 2056 203 1135 681 191 1471
Fragments arranged by size:
2056 1471 1135 681 219 203 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2589 2589
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 4060 4961 5223 820
Size: 197 926 783 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 2834 1517
Size: 1059 258 4639
Fragments arranged by size:
4639 1059 258
StyI C'CwwG_G
Cuts at: 513 1065 2004 2012 2593 2829 2839 3395 513
Size: 552 939 8 581 236 10 556 3074
Fragments arranged by size:
3074 939 581 556 552 236 10 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2345 2377 5916 5948 2279
Size: 32 34 32 3539 32 2287
Fragments arranged by size:
3539 2287 34 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2043 2082 2591 2043
Size: 39 509 5408
Fragments arranged by size:
5408 509 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI