Restriction map for
pFN21AE0430
(Linear) (Six-base) MAPSORT of: KIEE0430.seq Check: 9726 from: 1 to: 1251
KIEE0430.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 882 1251
Size: 882 369
AccI GT'mk_AC
Cuts at: 0 731 1251
Size: 731 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1251
Size: 436 815
AlwNI CAG_nnn'CTG
Cuts at: 0 399 670 1251
Size: 399 271 581
Fragments arranged by size:
581 399 271
ApaI G_GGCC'C
Cuts at: 0 381 449 711 1251
Size: 381 68 262 540
Fragments arranged by size:
540 381 262 68
ApaLI G'TGCA_C
Cuts at: 0 1127 1251
Size: 1127 124
ApoI r'AATT_y
Cuts at: 0 234 1251
Size: 234 1017
AvrII C'CTAG_G
Cuts at: 0 834 1251
Size: 834 417
BanI G'GyrC_C
Cuts at: 0 436 1251
Size: 436 815
BanII G_rGCy'C
Cuts at: 0 381 449 711 802 901 1251
Size: 381 68 262 91 99 350
Fragments arranged by size:
381 350 262 99 91 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 1085 1251
Size: 101 984 166
Fragments arranged by size:
984 166 101
BbvCI CC'TCA_GC
Cuts at: 0 49 122 1251
Size: 49 73 1129
Fragments arranged by size:
1129 73 49
BglI GCCn_nnn'nGGC
Cuts at: 0 434 554 584 1141 1251
Size: 434 120 30 557 110
Fragments arranged by size:
557 434 120 110 30
Bme1580I G_kGCm'C
Cuts at: 0 381 449 539 657 711 1131 1251
Size: 381 68 90 118 54 420 120
Fragments arranged by size:
420 381 120 118 90 68 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 1251
Size: 99 1152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 199 301 346 1251
Size: 70 129 102 45 905
Fragments arranged by size:
905 129 102 70 45
Bpu10I CC'TnA_GC
Cuts at: 0 49 122 663 1148 1251
Size: 49 73 541 485 103
Fragments arranged by size:
541 485 103 73 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1251
Size: 706 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1251
Size: 253 998
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 340 370 1251
Size: 274 30 36 30 881
Fragments arranged by size:
881 274 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 1251
Size: 352 899
BseYI C'CCAG_C
Cuts at: 0 30 704 1251
Size: 30 674 547
Fragments arranged by size:
674 547 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1251
Size: 312 939
BsiHKAI G_wGCw'C
Cuts at: 0 41 406 1131 1251
Size: 41 365 725 120
Fragments arranged by size:
725 365 120 41
Bsp1286I G_dGCh'C
Cuts at: 0 41 381 406 449 539 657 711 802
Size: 41 340 25 43 90 118 54 91
Cuts at: 802 901 1131 1251
Size: 99 230 120
Fragments arranged by size:
340 230 120 118 99 91 90 54
43 41 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 151 882 1251
Size: 151 731 369
Fragments arranged by size:
731 369 151
BsrDI GCAATG_nn'
Cuts at: 0 697 1251
Size: 697 554
BsrFI r'CCGG_y
Cuts at: 0 553 1251
Size: 553 698
BssSI C'ACGA_G
Cuts at: 0 521 1251
Size: 521 730
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 1251
Size: 624 627
BstYI r'GATC_y
Cuts at: 0 1040 1251
Size: 1040 211
Bsu36I CC'TnA_GG
Cuts at: 0 671 1251
Size: 671 580
BtgI C'CryG_G
Cuts at: 0 7 1251
Size: 7 1244
BtsI GCAGTG_nn'
Cuts at: 0 215 508 964 1230 1251
Size: 215 293 456 266 21
Fragments arranged by size:
456 293 266 215 21
EaeI y'GGCC_r
Cuts at: 0 576 1133 1193 1251
Size: 576 557 60 58
Fragments arranged by size:
576 557 60 58
EarI CTCTTCn'nnn_
Cuts at: 0 61 224 394 1251
Size: 61 163 170 857
Fragments arranged by size:
857 170 163 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 199 301 346 436 1251
Size: 70 129 102 45 90 815
Fragments arranged by size:
815 129 102 90 70 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 1251
Size: 271 980
EcoO109I rG'GnC_Cy
Cuts at: 0 21 45 73 174 377 378 446 482
Size: 21 24 28 101 203 1 68 36
Cuts at: 482 667 708 1251
Size: 185 41 543
Fragments arranged by size:
543 203 185 101 68 41 36 28
24 21 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 1173 1251
Size: 321 852 78
Fragments arranged by size:
852 321 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 1251
Size: 223 32 996
Fragments arranged by size:
996 223 32
HincII GTy'rAC
Cuts at: 0 159 1251
Size: 159 1092
MscI TGG'CCA
Cuts at: 0 578 1135 1251
Size: 578 557 116
Fragments arranged by size:
578 557 116
MslI CAynn'nnrTG
Cuts at: 0 1027 1251
Size: 1027 224
MspA1I CmG'CkG
Cuts at: 0 34 533 1251
Size: 34 499 718
Fragments arranged by size:
718 499 34
NaeI GCC'GGC
Cuts at: 0 555 1251
Size: 555 696
NcoI C'CATG_G
Cuts at: 0 7 1251
Size: 7 1244
NgoMIV G'CCGG_C
Cuts at: 0 553 1251
Size: 553 698
PfoI T'CCnGG_A
Cuts at: 0 191 942 1251
Size: 191 751 309
Fragments arranged by size:
751 309 191
PpuMI rG'GwC_Cy
Cuts at: 0 73 667 1251
Size: 73 594 584
Fragments arranged by size:
594 584 73
PspOMI G'GGCC_C
Cuts at: 0 377 445 707 1251
Size: 377 68 262 544
Fragments arranged by size:
544 377 262 68
PstI C_TGCA'G
Cuts at: 0 1100 1251
Size: 1100 151
PvuII CAG'CTG
Cuts at: 0 34 1251
Size: 34 1217
SfcI C'TryA_G
Cuts at: 0 1096 1251
Size: 1096 155
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 584 1251
Size: 584 667
SmlI C'TyrA_G
Cuts at: 0 721 1251
Size: 721 530
StuI AGG'CCT
Cuts at: 0 571 829 1251
Size: 571 258 422
Fragments arranged by size:
571 422 258
StyI C'CwwG_G
Cuts at: 0 7 588 824 834 1251
Size: 7 581 236 10 417
Fragments arranged by size:
581 417 236 10 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 340 372 1251
Size: 274 32 34 32 879
Fragments arranged by size:
879 274 34 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 38 77 586 1251
Size: 38 39 509 665
Fragments arranged by size:
665 509 39 38
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApaLI ApoI AvrII
BanI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV
PfoI PpuMI PspOMI PstI PvuII SfcI SfiI SmlI
StuI StyI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI