Restriction map for
pFN21AB5956
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BamHI
BbeI BbsI BbvCI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Van91I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AlwNI 445 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 621 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1255 CACGTG CAC^GTG
BcgI 167 GCAGATGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 593 TGATCA T^GATCA
BfaI 383 CTAG C^TAG
BsaAI 1255 CACGTG [CT]AC^GT[GA]
BsaBI 429 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 1616 ACCTGC ACCTGC(4/8)
BsrDI 1418 GCAATG GCAATG(2/0)
FbaI 593 TGATCA T^GATCA
HincII 1249 GTCAAC GT[CT]^[GA]AC
HindII 1249 GTCAAC GT[CT]^[GA]AC
MaeI 383 CTAG C^TAG
MaeII 1256 ACGT A^CGT
MamI 429 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 764 CTCGAG C^TCGAG
PmaCI 1255 CACGTG CAC^GTG
PmlI 1255 CACGTG CAC^GTG
PpuMI 506 AGGACCT [GA]G^G[AT]CC[CT]
PstI 345 CTGCAG CTGCA^G
ScaI 1304 AGTACT AGT^ACT
SfcI 345 CTGCAG C^T[GA][CT]AG
StuI 385 AGGCCT AGG^CCT
Tsp509I 1234 AATT ^AATT
XhoI 764 CTCGAG C^TCGAG
XmnI 621 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AlwI 857 GATCC GGATC(4/5)
173 GGATC
ApaI 51 GGGCCC GGGCC^C
1091 GGGCCC
AspI 769 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACGGGGTC
AvaI 764 CTCGAG C^[CT]CG[GA]G
837 CTCGGG
BalI 93 TGGCCA TGG^CCA
1556 TGGCCA
BanI 22 GGTGCC G^G[CT][GA]CC
1000 GGCACC
BcnI 111 CCGGG CC^[GC]GG
1262 CCCGG
BglI 479 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1021 GCCATCTGGGC
BsaI 1549 GAGACC GGTCTC(1/5)
1116 GGTCTC
BsiEI 359 CGATCG CG[GA][CT]^CG
434 CGATCG
BsmBI 363 CGTCTC CGTCTC(1/5)
554 GAGACG
BsoBI 764 CTCGAG C^[CT]CG[GA]G
837 CTCGGG
CfoI 280 GCGC GCG^C
1470 GCGC
HapII 111 CCGG C^CGG
1263 CCGG
HgaI 464 GCGTC GACGC(5/10)
773 GCGTC
HhaI 280 GCGC GCG^C
1470 GCGC
HinP1I 280 GCGC G^CGC
1470 GCGC
HinfI 957 GAATC G^A[ACGT]TC
1085 GATTC
HpaII 111 CCGG C^CGG
1263 CCGG
HphI 591 GGTGA GGTGA(8/7)
923 TCACC
MluNI 93 TGGCCA TGG^CCA
1556 TGGCCA
MscI 93 TGGCCA TGG^CCA
1556 TGGCCA
MslI 749 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1401 CATCATCGTG
MspI 111 CCGG C^CGG
1263 CCGG
NciI 111 CCGGG CC^[GC]GG
1262 CCCGG
NcoI 47 CCATGG C^CATGG
1436 CCATGG
NspI 1362 GCATGC [GA]CATG^[CT]
1463 GCATGC
PflFI 769 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACGGGGTC
PvuI 359 CGATCG CGAT^CG
434 CGATCG
SmlI 271 CTCAAG C^T[CT][GA]AG
764 CTCGAG
SphI 1362 GCATGC GCATG^C
1463 GCATGC
TfiI 957 GAATC G^A[AT]TC
1085 GATTC
Tsp45I 747 GTCAC ^GT[GC]AC
1343 GTGAC
Tth111I 769 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACGGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII ItaI MaeIII MboI
MboII MnlI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I XcmI