(Circular) (Six-base) MAPSORT of: pFN21AB5956.seq Check: 9572 from: 1 to: 6349 pFN21AB5956 6349 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4412 5796 6064 1632 Size: 2780 1384 268 1917 Fragments arranged by size: 2780 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 2758 1166 Size: 1592 4757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2591 1184 Size: 1407 4942 AlwNI CAG_nnn'CTG Cuts at: 2455 4092 5664 2455 Size: 1637 1572 3140 Fragments arranged by size: 3140 1637 1572 ApaI G_GGCC'C Cuts at: 2060 3100 2060 Size: 1040 5309 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2769 2842 3674 1870 Size: 73 826 73 832 4545 Fragments arranged by size: 4545 832 826 73 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 3005 3670 5125 618 Size: 325 217 627 240 978 665 1455 1842 Fragments arranged by size: 1842 1455 978 665 627 325 240 217 BanII G_rGCy'C Cuts at: 1391 2060 3100 3645 3668 1391 Size: 669 1040 545 23 4072 Fragments arranged by size: 4072 1040 669 545 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2161 2195 4540 4574 1903 Size: 34 224 34 2345 34 3678 Fragments arranged by size: 3678 2345 224 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 2598 1548 Size: 1050 5299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2490 3032 4960 Size: 107 122 71 631 605 818 542 1928 Cuts at: 4960 136 Size: 1525 Fragments arranged by size: 1928 1525 818 631 605 542 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2032 2060 2363 2643 3008 3100 4410 5566 Size: 242 28 303 280 365 92 1310 1156 Cuts at: 5566 1790 Size: 2573 Fragments arranged by size: 2573 1310 1156 365 303 280 242 92 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2013 2056 3094 Size: 416 53 83 399 24 596 43 1038 Cuts at: 3094 4080 5038 442 Size: 986 958 1753 Fragments arranged by size: 1753 1038 986 958 596 416 399 83 53 43 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 2561 2807 5009 1239 Size: 1117 205 246 2202 2579 Fragments arranged by size: 2579 2202 1117 246 205 Bpu10I CC'TnA_GC Cuts at: 3169 3169 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 4474 5339 5637 2261 Size: 2213 865 298 2973 Fragments arranged by size: 2973 2213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3127 3548 5012 882 Size: 617 1628 421 1464 2219 Fragments arranged by size: 2219 1628 1464 617 421 BsaAI yAC'GTr Cuts at: 493 1204 3262 4140 493 Size: 711 2058 878 2702 Fragments arranged by size: 2702 2058 878 711 BsaBI GATnn'nnATC Cuts at: 2438 6343 2438 Size: 3905 2444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 1178 Size: 30 165 30 1377 30 4717 Fragments arranged by size: 4717 1377 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 3311 3371 3506 1186 Size: 1090 1035 60 135 4029 Fragments arranged by size: 4029 1090 1035 135 60 BseYI C'CCAG_C Cuts at: 2032 5552 6048 2032 Size: 3520 496 2333 Fragments arranged by size: 3520 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3398 3533 3620 1163 Size: 573 1662 135 87 3892 Fragments arranged by size: 3892 1662 573 135 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2367 2442 3799 4560 5588 1540 Size: 275 186 366 75 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 366 275 186 75 BsiHKAI G_wGCw'C Cuts at: 4410 4495 5566 4410 Size: 85 1071 5193 Fragments arranged by size: 5193 1071 85 BsmI GAATG_Cn' Cuts at: 3860 3953 3860 Size: 93 6256 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 2553 2374 Size: 179 6170 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2060 2363 2643 3008 3100 3645 Size: 399 242 28 303 280 365 92 545 Cuts at: 3645 3668 4410 4495 5566 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 365 303 280 242 92 85 28 23 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3630 3704 844 Size: 2786 74 3489 Fragments arranged by size: 3489 2786 74 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3430 4844 5018 65 Size: 1183 2182 1414 174 1396 Fragments arranged by size: 2182 1414 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstYI r'GATC_y Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344 Size: 1707 367 385 17 1452 86 12 346 Cuts at: 6344 1972 Size: 1977 Fragments arranged by size: 1977 1707 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 3372 3441 513 Size: 552 939 48 1320 69 3421 Fragments arranged by size: 3421 1320 939 552 69 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 3865 4659 4679 3865 Size: 794 20 5535 Fragments arranged by size: 5535 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2468 2749 3119 5356 809 Size: 507 1152 281 370 2237 1802 Fragments arranged by size: 2237 1802 1152 507 370 281 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3024 3300 Size: 54 1425 50 275 18 268 926 276 Cuts at: 3300 3561 3796 4685 6314 8 Size: 261 235 889 1629 43 Fragments arranged by size: 1629 1425 926 889 276 275 268 261 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4279 1353 Size: 2926 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3593 4936 5310 5456 387 Size: 1064 138 2004 1343 374 146 1280 Fragments arranged by size: 2004 1343 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2561 2807 4412 5009 5796 6064 Size: 393 724 205 246 1605 597 787 268 Cuts at: 6064 1239 Size: 1524 Fragments arranged by size: 1605 1524 787 724 597 393 268 246 205 EcoO109I rG'GnC_Cy Cuts at: 2057 2512 3096 2057 Size: 455 584 5310 Fragments arranged by size: 5310 584 455 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2533 2533 Size: 6349 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2763 2795 2963 Size: 32 135 32 539 32 620 32 168 Cuts at: 2963 2995 4990 5022 5064 5096 1373 Size: 32 1995 32 42 32 2626 Fragments arranged by size: 2626 1995 620 539 168 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3256 3693 3939 669 Size: 988 281 1318 437 246 3079 Fragments arranged by size: 3079 1318 988 437 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 2484 2914 2979 5463 5647 1305 Size: 855 324 430 65 2484 184 2007 Fragments arranged by size: 2484 2007 855 430 324 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 3563 10 Size: 54 1425 343 268 1463 2796 Fragments arranged by size: 2796 1463 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 2758 3410 4307 4666 4825 518 Size: 648 1592 652 897 359 159 2042 Fragments arranged by size: 2042 1592 897 652 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2785 3295 3765 4442 5590 5835 1606 Size: 433 746 510 470 677 1148 245 2120 Fragments arranged by size: 2120 1148 746 677 510 470 433 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 3441 513 Size: 552 939 48 1389 3421 Fragments arranged by size: 3421 1389 939 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3371 3472 3707 5252 1132 Size: 97 2142 101 235 1545 2229 Fragments arranged by size: 2229 2142 1545 235 101 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PfoI T'CCnGG_A Cuts at: 3450 3450 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 3262 3262 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2512 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 2550 3919 2550 Size: 1369 4980 PspOMI G'GGCC_C Cuts at: 2056 3096 2056 Size: 1040 5309 PstI C_TGCA'G Cuts at: 830 2354 3701 830 Size: 1524 1347 3478 Fragments arranged by size: 3478 1524 1347 PvuI CG_AT'CG Cuts at: 2001 2367 2442 2001 Size: 366 75 5908 Fragments arranged by size: 5908 366 75 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 3311 4597 6309 3311 Size: 1286 1712 3351 Fragments arranged by size: 3351 1712 1286 SfcI C'TryA_G Cuts at: 826 1045 2350 3697 4832 5513 5704 826 Size: 219 1305 1347 1135 681 191 1471 Fragments arranged by size: 1471 1347 1305 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2769 4453 5354 5616 820 Size: 197 926 333 493 1684 901 262 1553 Fragments arranged by size: 1684 1553 926 901 493 333 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3371 3472 3707 1132 Size: 2239 101 235 3774 Fragments arranged by size: 3774 2239 235 101 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 2392 1517 Size: 875 5474 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2643 3441 3558 3788 513 Size: 552 939 48 591 798 117 230 3074 Fragments arranged by size: 3074 939 798 591 552 230 117 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4376 4393 4546 4731 5176 325 Size: 1255 2796 17 153 185 445 1498 Fragments arranged by size: 2796 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2604 3309 4595 Size: 274 80 33 51 1443 627 705 1286 Cuts at: 4595 6307 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1286 705 627 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2606 2638 2780 2812 6309 6341 2606 Size: 32 142 32 3497 32 2614 Fragments arranged by size: 3497 2614 142 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2777 3338 1316 Size: 1461 561 4327 Fragments arranged by size: 4327 1461 561 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2018 2314 3409 3448 3571 2018 Size: 296 1095 39 123 4796 Fragments arranged by size: 4796 1095 296 123 39 XhoI C'TCGA_G Cuts at: 1943 2769 1943 Size: 826 5523 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2630 4478 2630 Size: 1848 4501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI