Restriction map for
pFN21AB5956
(Linear) (Six-base) MAPSORT of: KIBB5956.seq Check: 7992 from: 1 to: 1644
KIBB5956.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AleI CACnn'nnGTG
Cuts at: 0 753 1644
Size: 753 891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 1644
Size: 586 1058
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1644
Size: 450 1194
ApaI G_GGCC'C
Cuts at: 0 55 1095 1644
Size: 55 1040 549
Fragments arranged by size:
1040 549 55
AvaI C'yCGr_G
Cuts at: 0 764 837 1644
Size: 764 73 807
Fragments arranged by size:
807 764 73
BanI G'GyrC_C
Cuts at: 0 22 1000 1644
Size: 22 978 644
Fragments arranged by size:
978 644 22
BanII G_rGCy'C
Cuts at: 0 55 1095 1640 1644
Size: 55 1040 545 4
Fragments arranged by size:
1040 545 55 4
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 156 190 1644
Size: 156 34 1454
Fragments arranged by size:
1454 156 34
BclI T'GATC_A
Cuts at: 0 593 1644
Size: 593 1051
BglI GCCn_nnn'nGGC
Cuts at: 0 485 1027 1644
Size: 485 542 617
Fragments arranged by size:
617 542 485
Bme1580I G_kGCm'C
Cuts at: 0 27 55 358 638 1003 1095 1644
Size: 27 28 303 280 365 92 549
Fragments arranged by size:
549 365 303 280 92 28 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 8 51 1089 1644
Size: 8 43 1038 555
Fragments arranged by size:
1038 555 43 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 556 802 1644
Size: 351 205 246 842
Fragments arranged by size:
842 351 246 205
Bpu10I CC'TnA_GC
Cuts at: 0 1164 1644
Size: 1164 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1644
Size: 256 1388
BsaI GGTCTCn'nnnn_
Cuts at: 0 1122 1543 1644
Size: 1122 421 101
Fragments arranged by size:
1122 421 101
BsaAI yAC'GTr
Cuts at: 0 1257 1644
Size: 1257 387
BsaBI GATnn'nnATC
Cuts at: 0 433 1644
Size: 433 1211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1644
Size: 775 30 839
Fragments arranged by size:
839 775 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 1306 1366 1501 1644
Size: 271 1035 60 135 143
Fragments arranged by size:
1035 271 143 135 60
BseYI C'CCAG_C
Cuts at: 0 27 1644
Size: 27 1617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1528 1615 1644
Size: 1393 135 87 29
Fragments arranged by size:
1393 135 87 29
BsiEI CG_ry'CG
Cuts at: 0 362 437 1644
Size: 362 75 1207
Fragments arranged by size:
1207 362 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 548 1644
Size: 369 179 1096
Fragments arranged by size:
1096 369 179
Bsp1286I G_dGCh'C
Cuts at: 0 27 55 358 638 1003 1095 1640 1644
Size: 27 28 303 280 365 92 545 4
Fragments arranged by size:
545 365 303 280 92 28 27 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1625 1644
Size: 1625 19
BsrDI GCAATG_nn'
Cuts at: 0 1425 1644
Size: 1425 219
BtgI C'CryG_G
Cuts at: 0 47 1367 1436 1644
Size: 47 1320 69 208
Fragments arranged by size:
1320 208 69 47
DrdI GACnn_nn'nnGTC
Cuts at: 0 463 744 1114 1644
Size: 463 281 370 530
Fragments arranged by size:
530 463 370 281
EaeI y'GGCC_r
Cuts at: 0 93 1019 1295 1556 1644
Size: 93 926 276 261 88
Fragments arranged by size:
926 276 261 93 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1588 1644
Size: 1588 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 556 802 1644
Size: 351 205 246 842
Fragments arranged by size:
842 351 246 205
EcoO109I rG'GnC_Cy
Cuts at: 0 52 507 1091 1644
Size: 52 455 584 553
Fragments arranged by size:
584 553 455 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 528 1644
Size: 528 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 758 790 958 990 1644
Size: 106 32 620 32 168 32 654
Fragments arranged by size:
654 620 168 106 32 32 32
HincII GTy'rAC
Cuts at: 0 1251 1644
Size: 1251 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 479 909 974 1644
Size: 155 324 430 65 670
Fragments arranged by size:
670 430 324 155 65
MscI TGG'CCA
Cuts at: 0 95 1558 1644
Size: 95 1463 86
Fragments arranged by size:
1463 95 86
MslI CAynn'nnrTG
Cuts at: 0 753 1405 1644
Size: 753 652 239
Fragments arranged by size:
753 652 239
MspA1I CmG'CkG
Cuts at: 0 34 780 1290 1644
Size: 34 746 510 354
Fragments arranged by size:
746 510 354 34
NcoI C'CATG_G
Cuts at: 0 47 1436 1644
Size: 47 1389 208
Fragments arranged by size:
1389 208 47
NspI r_CATG'y
Cuts at: 0 1366 1467 1644
Size: 1366 101 177
Fragments arranged by size:
1366 177 101
PfoI T'CCnGG_A
Cuts at: 0 1445 1644
Size: 1445 199
PmlI CAC'GTG
Cuts at: 0 1257 1644
Size: 1257 387
PpuMI rG'GwC_Cy
Cuts at: 0 507 1644
Size: 507 1137
PsiI TTA'TAA
Cuts at: 0 545 1644
Size: 545 1099
PspOMI G'GGCC_C
Cuts at: 0 51 1091 1644
Size: 51 1040 553
Fragments arranged by size:
1040 553 51
PstI C_TGCA'G
Cuts at: 0 349 1644
Size: 349 1295
PvuI CG_AT'CG
Cuts at: 0 362 437 1644
Size: 362 75 1207
Fragments arranged by size:
1207 362 75
ScaI AGT'ACT
Cuts at: 0 1306 1644
Size: 1306 338
SfcI C'TryA_G
Cuts at: 0 345 1644
Size: 345 1299
SmlI C'TyrA_G
Cuts at: 0 271 764 1644
Size: 271 493 880
Fragments arranged by size:
880 493 271
SphI G_CATG'C
Cuts at: 0 1366 1467 1644
Size: 1366 101 177
Fragments arranged by size:
1366 177 101
StuI AGG'CCT
Cuts at: 0 387 1644
Size: 387 1257
StyI C'CwwG_G
Cuts at: 0 47 638 1436 1553 1644
Size: 47 591 798 117 91
Fragments arranged by size:
798 591 117 91 47
TatI w'GTAC_w
Cuts at: 0 599 1304 1644
Size: 599 705 340
Fragments arranged by size:
705 599 340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 775 807 1644
Size: 601 32 142 32 837
Fragments arranged by size:
837 601 142 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 772 1333 1644
Size: 772 561 311
Fragments arranged by size:
772 561 311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 13 309 1404 1443 1566 1644
Size: 13 296 1095 39 123 78
Fragments arranged by size:
1095 296 123 78 39 13
XhoI C'TCGA_G
Cuts at: 0 764 1644
Size: 764 880
XmnI GAAnn'nnTTC
Cuts at: 0 625 1644
Size: 625 1019
Enzymes that do cut:
AleI AlfI AlwNI ApaI AvaI BanI BanII BcgI
BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrDI BtgI DrdI EaeI EciI Eco57MI
EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I
NcoI NspI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuI ScaI SfcI SmlI SphI StuI StyI TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BsaHI BsaWI
BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PshAI PspXI PsrI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII XbaI XmaI ZraI