(Circular) (Six-base) MAPSORT of: pFN21AE0122.seq Check: 6231 from: 1 to: 5500 pFN21AE0122 5500 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3310 278 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2843 1937 Size: 906 4594 Acc65I G'GTAC_C Cuts at: 2478 2821 2478 Size: 343 5157 AclI AA'CG_TT Cuts at: 1563 3627 4000 1563 Size: 2064 373 3063 Fragments arranged by size: 3063 2064 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 3563 4947 5215 1632 Size: 638 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 638 268 AfeI AGC'GCT Cuts at: 2154 2154 Size: 5500 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5303 AflIII A'CryG_T Cuts at: 1203 4399 5376 1203 Size: 3196 977 1327 Fragments arranged by size: 3196 1327 977 AgeI A'CCGG_T Cuts at: 4331 4331 Size: 5500 AhdI GACnn_n'nnGTC Cuts at: 4229 4229 Size: 5500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5500 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 5468 AlwNI CAG_nnn'CTG Cuts at: 2299 2356 2635 2737 3243 4815 2299 Size: 57 279 102 506 1572 2984 Fragments arranged by size: 2984 1572 506 279 102 57 ApaLI G'TGCA_C Cuts at: 2489 3557 4713 2489 Size: 1068 1156 3276 Fragments arranged by size: 3276 1156 1068 ApoI r'AATT_y Cuts at: 1493 2809 3035 3187 5296 1493 Size: 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 AseI AT'TA_AT Cuts at: 160 1032 4054 160 Size: 872 3022 1606 Fragments arranged by size: 3022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2424 2825 1870 Size: 73 107 374 401 4545 Fragments arranged by size: 4545 401 374 107 73 BamHI G'GATC_C Cuts at: 2278 2830 3197 2278 Size: 552 367 4581 Fragments arranged by size: 4581 552 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2288 2478 2821 4276 Size: 325 217 627 315 186 190 343 1455 Cuts at: 4276 618 Size: 1842 Fragments arranged by size: 1842 1455 627 343 325 315 217 190 186 BanII G_rGCy'C Cuts at: 1391 2049 2692 2746 2819 1391 Size: 658 643 54 73 4072 Fragments arranged by size: 4072 658 643 73 54 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1999 2033 3691 3725 1903 Size: 34 62 34 1658 34 3678 Fragments arranged by size: 3678 1658 62 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3392 4602 3392 Size: 1210 4290 BclI T'GATC_A Cuts at: 1548 1548 Size: 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4111 136 Size: 107 122 71 631 605 2439 1525 Fragments arranged by size: 2439 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5495 5495 Size: 5500 BlpI GC'TnA_GC Cuts at: 2917 2917 Size: 5500 Bme1580I G_kGCm'C Cuts at: 1790 2293 2493 3561 4717 1790 Size: 503 200 1068 1156 2573 Fragments arranged by size: 2573 1156 1068 503 200 BmgBI CAC'GTC Cuts at: 1318 2785 1318 Size: 1467 4033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3231 4189 442 Size: 416 53 83 399 24 1814 958 1753 Fragments arranged by size: 1814 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2462 1056 Size: 1406 4094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 4160 1239 Size: 1094 1827 2579 Fragments arranged by size: 2579 1827 1094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3625 4490 4788 3625 Size: 865 298 4337 Fragments arranged by size: 4337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 2416 2653 4163 882 Size: 617 723 194 237 1510 2219 Fragments arranged by size: 2219 1510 723 617 237 194 BsaAI yAC'GTr Cuts at: 493 1204 3291 493 Size: 711 2087 2702 Fragments arranged by size: 2702 2087 711 BsaBI GATnn'nnATC Cuts at: 5494 5494 Size: 5500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3307 3689 4324 Size: 53 83 186 936 570 1204 382 635 Cuts at: 4324 275 Size: 1451 Fragments arranged by size: 1451 1204 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3933 4331 4605 4752 5163 3933 Size: 398 274 147 411 4270 Fragments arranged by size: 4270 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 2447 2477 1178 Size: 30 165 30 993 30 21 30 4201 Fragments arranged by size: 4201 993 165 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2287 2468 2768 1186 Size: 1101 181 300 3918 Fragments arranged by size: 3918 1101 300 181 BseYI C'CCAG_C Cuts at: 2512 2724 2758 4703 5199 2512 Size: 212 34 1945 496 2813 Fragments arranged by size: 2813 1945 496 212 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4927 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2377 2950 3711 4739 1540 Size: 275 186 376 573 761 1028 2301 Fragments arranged by size: 2301 1028 761 573 376 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 2493 3561 3646 4717 2431 Size: 62 1068 85 1071 3214 Fragments arranged by size: 3214 1071 1068 85 62 BsmI GAATG_Cn' Cuts at: 2600 3011 3104 2600 Size: 411 93 4996 Fragments arranged by size: 4996 411 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2293 2431 2493 2692 2746 2819 Size: 399 259 244 138 62 199 54 73 Cuts at: 2819 3561 3646 4717 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 244 199 138 85 73 62 54 BspEI T'CCGG_A Cuts at: 5163 5163 Size: 5500 BspHI T'CATG_A Cuts at: 3389 5119 3389 Size: 1730 3770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 844 Size: 2011 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2719 3387 1394 Size: 429 896 668 3507 Fragments arranged by size: 3507 896 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3995 4169 65 Size: 1183 2747 174 1396 Fragments arranged by size: 2747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 2165 4144 4331 1700 Size: 110 295 60 1979 187 2869 Fragments arranged by size: 2869 1979 295 187 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5500 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5500 BssSI C'ACGA_G Cuts at: 3560 4572 3560 Size: 1012 4488 BstAPI GCAn_nnn'nTGC Cuts at: 794 2497 794 Size: 1703 3797 BstBI TT'CG_AA Cuts at: 4304 4304 Size: 5500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5500 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 5500 BstYI r'GATC_y Cuts at: 1972 2278 2295 2830 3197 3582 3599 5051 5137 Size: 306 17 535 367 385 17 1452 86 Cuts at: 5137 5149 5495 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 306 86 17 17 12 Bsu36I CC'TnA_GG Cuts at: 2258 2738 2258 Size: 480 5020 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2663 513 Size: 552 939 168 491 3350 Fragments arranged by size: 3350 939 552 491 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2192 487 Size: 683 56 966 3795 Fragments arranged by size: 3795 966 683 56 BtsI GCAGTG_nn' Cuts at: 3016 3810 3830 3016 Size: 794 20 4686 Fragments arranged by size: 4686 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5465 DraI TTT'AAA Cuts at: 2804 2804 Size: 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4507 809 Size: 507 3191 1802 Fragments arranged by size: 3191 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2796 2947 Size: 54 1425 50 275 18 330 636 151 Cuts at: 2947 3836 5465 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 636 330 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2947 1537 Size: 275 1135 4090 Fragments arranged by size: 4090 1135 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3430 1353 Size: 2077 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4087 4461 4607 387 Size: 1064 138 2498 374 146 1280 Fragments arranged by size: 2498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2333 3563 4160 4947 5215 1239 Size: 393 638 63 1230 597 787 268 1524 Fragments arranged by size: 1524 1230 787 638 597 393 268 63 EcoO109I rG'GnC_Cy Cuts at: 2254 2254 Size: 5500 EcoRI G'AATT_C Cuts at: 2809 3187 2809 Size: 378 5122 FspI TGC'GCA Cuts at: 4006 4006 Size: 5500 HaeII r_GCGC'y Cuts at: 1678 2106 2156 2186 2628 2725 4647 1678 Size: 428 50 30 442 97 1922 2531 Fragments arranged by size: 2531 1922 442 428 97 50 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2263 2295 4141 4173 4215 Size: 32 135 32 691 32 1846 32 42 Cuts at: 4215 4247 1373 Size: 32 2626 Fragments arranged by size: 2626 1846 691 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2844 3090 669 Size: 988 281 906 246 3079 Fragments arranged by size: 3079 988 906 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5500 HpaI GTT'AAC Cuts at: 3090 3090 Size: 5500 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5500 KpnI G_GTAC'C Cuts at: 2482 2825 2482 Size: 343 5157 MfeI C'AATT_G Cuts at: 3099 3099 Size: 5500 MluI A'CGCG_T Cuts at: 5376 5376 Size: 5500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 4614 4798 1305 Size: 1336 1973 184 2007 Fragments arranged by size: 2007 1973 1336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3678 Fragments arranged by size: 3678 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3458 3817 3976 518 Size: 648 2292 359 159 2042 Fragments arranged by size: 2292 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2143 2174 2476 2916 3593 4741 4986 Size: 456 81 31 302 440 677 1148 245 Cuts at: 4986 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 456 440 302 245 81 31 NaeI GCC'GGC Cuts at: 2107 2167 2107 Size: 60 5440 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5500 NcoI C'CATG_G Cuts at: 513 1065 2004 2663 513 Size: 552 939 659 3350 Fragments arranged by size: 3350 939 659 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5500 NgoMIV G'CCGG_C Cuts at: 2105 2165 2105 Size: 60 5440 NheI G'CTAG_C Cuts at: 1052 2458 1052 Size: 1406 4094 NotI GC'GGCC_GC Cuts at: 2947 2947 Size: 5500 NruI TCG'CGA Cuts at: 2379 2379 Size: 5500 NspI r_CATG'y Cuts at: 1132 1229 2500 2858 4403 1132 Size: 97 1271 358 1545 2229 Fragments arranged by size: 2229 1545 1271 358 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5500 PciI A'CATG_T Cuts at: 4399 4399 Size: 5500 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 2794 1108 Size: 1101 585 3814 Fragments arranged by size: 3814 1101 585 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5500 PmeI GTTT'AAAC Cuts at: 2804 2804 Size: 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2428 3569 3601 5082 5114 2396 Size: 32 1141 32 1481 32 2782 Fragments arranged by size: 2782 1481 1141 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2254 Size: 5500 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5500 PsiI TTA'TAA Cuts at: 3070 3070 Size: 5500 PstI C_TGCA'G Cuts at: 830 2148 2304 2852 830 Size: 1318 156 548 3478 Fragments arranged by size: 3478 1318 548 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5500 SacII CC_GC'GG Cuts at: 2175 2175 Size: 5500 SalI G'TCGA_C Cuts at: 1936 2842 1936 Size: 906 4594 SbfI CC_TGCA'GG Cuts at: 2304 2852 2304 Size: 548 4952 ScaI AGT'ACT Cuts at: 3748 5460 3748 Size: 1712 3788 SfcI C'TryA_G Cuts at: 826 1045 2057 2144 2300 2848 3983 4664 4855 Size: 219 1012 87 156 548 1135 681 191 Cuts at: 4855 826 Size: 1471 Fragments arranged by size: 1471 1135 1012 681 548 219 191 156 87 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5500 SmaI CCC'GGG Cuts at: 1872 2052 2827 1872 Size: 180 775 4545 Fragments arranged by size: 4545 775 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 3604 4505 4767 820 Size: 197 926 1661 901 262 1553 Fragments arranged by size: 1661 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3291 493 Size: 2798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5500 SphI G_CATG'C Cuts at: 1132 2858 1132 Size: 1726 3774 SspI AAT'ATT Cuts at: 5 52 3424 5 Size: 47 3372 2081 Fragments arranged by size: 3372 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5500 StyI C'CwwG_G Cuts at: 513 1065 2004 2663 2939 513 Size: 552 939 659 276 3074 Fragments arranged by size: 3074 939 659 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 2446 2585 2626 2791 3527 3544 Size: 1255 783 83 139 41 165 736 17 Cuts at: 3544 3697 3882 4327 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 783 736 445 185 165 153 139 83 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2709 3746 5458 Size: 274 80 33 51 1443 732 1037 1712 Cuts at: 5458 96 Size: 138 Fragments arranged by size: 1712 1443 1037 732 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 5468 Tth111I GACn'n_nGTC Cuts at: 1316 2210 1316 Size: 894 4606 XbaI T'CTAG_A Cuts at: 2836 2836 Size: 5500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2335 2440 2530 2590 2335 Size: 105 90 60 5245 Fragments arranged by size: 5245 105 90 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5500 XmaI C'CCGG_G Cuts at: 1870 2050 2825 1870 Size: 180 775 4545 Fragments arranged by size: 4545 775 180 XmnI GAAnn'nnTTC Cuts at: 3629 3629 Size: 5500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3308 276 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI