Restriction map for
pFN21AE0122
(Linear) (Six-base) MAPSORT of: KIEE0122.seq Check: 3726 from: 1 to: 795
KIEE0122.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
Acc65I G'GTAC_C
Cuts at: 0 473 795
Size: 473 322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 795
Size: 265 530
AfeI AGC'GCT
Cuts at: 0 149 795
Size: 149 646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 795
Size: 391 32 372
Fragments arranged by size:
391 372 32
AlwNI CAG_nnn'CTG
Cuts at: 0 294 351 630 732 795
Size: 294 57 279 102 63
Fragments arranged by size:
294 279 102 63 57
ApaLI G'TGCA_C
Cuts at: 0 484 795
Size: 484 311
AvaI C'yCGr_G
Cuts at: 0 45 419 795
Size: 45 374 376
Fragments arranged by size:
376 374 45
BamHI G'GATC_C
Cuts at: 0 273 795
Size: 273 522
BanI G'GyrC_C
Cuts at: 0 97 283 473 795
Size: 97 186 190 322
Fragments arranged by size:
322 190 186 97
BanII G_rGCy'C
Cuts at: 0 44 687 741 795
Size: 44 643 54 54
Fragments arranged by size:
643 54 54 44
BbeI G_GCGC'C
Cuts at: 0 101 795
Size: 101 694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 795
Size: 28 767
Bme1580I G_kGCm'C
Cuts at: 0 288 488 795
Size: 288 200 307
Fragments arranged by size:
307 288 200
BmgBI CAC'GTC
Cuts at: 0 780 795
Size: 780 15
BmtI G_CTAG'C
Cuts at: 0 457 795
Size: 457 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 795
Size: 328 467
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 411 648 795
Size: 217 194 237 147
Fragments arranged by size:
237 217 194 147
BsaHI Gr'CG_yC
Cuts at: 0 98 795
Size: 98 697
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 442 472 795
Size: 391 30 21 30 323
Fragments arranged by size:
391 323 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 282 463 763 795
Size: 282 181 300 32
Fragments arranged by size:
300 282 181 32
BseYI C'CCAG_C
Cuts at: 0 507 719 753 795
Size: 507 212 34 42
Fragments arranged by size:
507 212 42 34
BsiEI CG_ry'CG
Cuts at: 0 372 795
Size: 372 423
BsiHKAI G_wGCw'C
Cuts at: 0 426 488 795
Size: 426 62 307
Fragments arranged by size:
426 307 62
BsmI GAATG_Cn'
Cuts at: 0 595 795
Size: 595 200
Bsp1286I G_dGCh'C
Cuts at: 0 44 288 426 488 687 741 795
Size: 44 244 138 62 199 54 54
Fragments arranged by size:
244 199 138 62 54 54 44
BsrBI CCG'CTC
Cuts at: 0 714 795
Size: 714 81
BsrFI r'CCGG_y
Cuts at: 0 100 160 795
Size: 100 60 635
Fragments arranged by size:
635 100 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 795
Size: 492 303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 795
Size: 528 267
BstYI r'GATC_y
Cuts at: 0 273 290 795
Size: 273 17 505
Fragments arranged by size:
505 273 17
Bsu36I CC'TnA_GG
Cuts at: 0 253 733 795
Size: 253 480 62
Fragments arranged by size:
480 253 62
BtgI C'CryG_G
Cuts at: 0 167 658 795
Size: 167 491 137
Fragments arranged by size:
491 167 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 187 795
Size: 187 608
EaeI y'GGCC_r
Cuts at: 0 155 795
Size: 155 640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 328 795
Size: 265 63 467
Fragments arranged by size:
467 265 63
EcoO109I rG'GnC_Cy
Cuts at: 0 249 795
Size: 249 546
HaeII r_GCGC'y
Cuts at: 0 101 151 181 623 720 795
Size: 101 50 30 442 97 75
Fragments arranged by size:
442 101 97 75 50 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 258 290 795
Size: 258 32 505
Fragments arranged by size:
505 258 32
KasI G'GCGC_C
Cuts at: 0 97 795
Size: 97 698
KpnI G_GTAC'C
Cuts at: 0 477 795
Size: 477 318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 795
Size: 636 159
MspA1I CmG'CkG
Cuts at: 0 57 138 169 471 795
Size: 57 81 31 302 324
Fragments arranged by size:
324 302 81 57 31
NaeI GCC'GGC
Cuts at: 0 102 162 795
Size: 102 60 633
Fragments arranged by size:
633 102 60
NarI GG'CG_CC
Cuts at: 0 98 795
Size: 98 697
NcoI C'CATG_G
Cuts at: 0 658 795
Size: 658 137
NgoMIV G'CCGG_C
Cuts at: 0 100 160 795
Size: 100 60 635
Fragments arranged by size:
635 100 60
NheI G'CTAG_C
Cuts at: 0 453 795
Size: 453 342
NruI TCG'CGA
Cuts at: 0 374 795
Size: 374 421
NspI r_CATG'y
Cuts at: 0 495 795
Size: 495 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 789 795
Size: 204 585 6
Fragments arranged by size:
585 204 6
PfoI T'CCnGG_A
Cuts at: 0 389 795
Size: 389 406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 795
Size: 391 32 372
Fragments arranged by size:
391 372 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 795
Size: 249 546
PstI C_TGCA'G
Cuts at: 0 143 299 795
Size: 143 156 496
Fragments arranged by size:
496 156 143
SacII CC_GC'GG
Cuts at: 0 170 795
Size: 170 625
SbfI CC_TGCA'GG
Cuts at: 0 299 795
Size: 299 496
SfcI C'TryA_G
Cuts at: 0 52 139 295 795
Size: 52 87 156 500
Fragments arranged by size:
500 156 87 52
SfoI GGC'GCC
Cuts at: 0 99 795
Size: 99 696
SmaI CCC'GGG
Cuts at: 0 47 795
Size: 47 748
StyI C'CwwG_G
Cuts at: 0 658 795
Size: 658 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 441 580 621 786 795
Size: 358 83 139 41 165 9
Fragments arranged by size:
358 165 139 83 41 9
TatI w'GTAC_w
Cuts at: 0 704 795
Size: 704 91
Tth111I GACn'n_nGTC
Cuts at: 0 205 795
Size: 205 590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 435 525 585 795
Size: 330 105 90 60 210
Fragments arranged by size:
330 210 105 90 60
XmaI C'CCGG_G
Cuts at: 0 45 795
Size: 45 750
Enzymes that do cut:
Acc65I AcuI AfeI AloI AlwNI ApaLI AvaI BamHI
BanI BanII BbeI BcgI Bme1580I BmgBI BmtI BpmI
BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI EaeI Eco57MI EcoO109I HaeII Hin4I KasI KpnI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PflMI PfoI PpiI PpuMI PstI SacII SbfI
SfcI SfoI SmaI StyI TaqII TatI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmrI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsgI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MscI MslI NdeI NotI NsiI
PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmnI ZraI